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We narrowed to 7,730 results for: PAC

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Showing: 7021 - 7040 of 7730 results

  2. pShHELIX(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT112)

    Plasmid
    #181791
    Purpose
    Expresses 3-component ShHELIX containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    181791-plasmid-map-sequence-id-354814

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  3. pDM026

    Plasmid
    #216808
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and pyrG selectable marker.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Expression
    Bacterial
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216808-plasmid-map-sequence-id-429385

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  6. pEC3111 (pSpy1C-Plac(3)_ffluc)

    Plasmid
    #218778
    Purpose
    replicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressor
    Depositor
    Emmanuelle Charpentier
    Article
    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1
    Insert
    ffluc
    Use
    Luciferase
    Expression
    Bacterial
    Mutation
    deletion of mrfp cassette, mutagenesis of the RBS…
    Promoter
    Plac(3)
    Available Since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    218778-plasmid-map-sequence-id-444099

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  9. pCW17 - pAdapter

    Plasmid
    #174664
    Purpose
    Constitutive expression of λCI fused via three alanines to the MS2 coat protein. Useful as RNA-DNA adapter in three-hybrid system.
    Depositor
    Katherine Berry
    Article
    Pandey et al Nucleic Acids Res. 2020 May 7;48(8):4507
    Insert
    MS2 coat protein
    Tags
    λCI
    Expression
    Bacterial
    Mutation
    V30I, A81G, positions 68-80 deleted (VATQTVGGVELP…
    Promoter
    Synthetic Constitutive Promoter
    Available Since
    May 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    174664-plasmid-map-sequence-id-361957

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  10. pMBEC8

    Plasmid
    #187599
    Purpose
    Base editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Apramycin resistance
    Depositor
    Pablo Ivan Nikel
    Article
    Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:
    Insert
    nCas9-BEC-UGI; cas6f, gfp
    Expression
    Bacterial
    Promoter
    trc
    Available Since
    July 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    187599-plasmid-map-sequence-id-367572

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  12. pMS20: pHelper(PmcCAST)_PmcPSP1_ΔTnsD

    Plasmid
    #168153
    Purpose
    Inducible expression of PmcCAST proteins (except TnsD) with crRNA targeting PmcPSP1.
    Depositor
    Feng Zhang
    Article
    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.
    Inserts
    PmcCAST minimal CRISPR array (with spacer for PmcPSP1)
    PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    PmcCAST Tns proteins (TnsAB and TnsC)
    PmcTniQ
    Expression
    Bacterial
    Available Since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    168153-plasmid-map-sequence-id-330740

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  15. pShCAST(Cas12k-TnsC)-sgRNA_entry (CJT28)

    Plasmid
    #181789
    Purpose
    Expresses 3-component ShCAST containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    ShTnsB, ShTniQ, ShCas12k-ShTnsC
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181789-plasmid-map-sequence-id-354816

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  18. pXPR-SF61

    Plasmid
    #163916
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of para-Pentafluorosulfanyl-Phenylalanine, into proteins in E. coli in response to the amber (TAG) codon
    Depositor
    Thomas Huber
    Article
    Qianzhu et al J Am Chem Soc. 2020 Oct 14;142(41):17277
    Insert
    SF5Phe tRNA synthetase
    Expression
    Bacterial
    Promoter
    araBAD
    Available Since
    March 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    163916-plasmid-map-sequence-id-327914

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  19. kiCAP-AAV-PAL1C

    Plasmid
    #196685
    Purpose
    Rep/Cap plasmid for the production of PAL1C, an AAV capsid with CNS tropism in macaques.
    Depositor
    Pardis Sabeti
    Article
    Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Mutation
    PTQGTLR insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available Since
    April 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    196685-plasmid-map-sequence-id-389718

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  20. p35u4

    Plasmid
    #174786
    Purpose
    Constitutive expression of λCI fused via three alanines to the MS2 coat protein. Useful as RNA-DNA adapter in three-hybrid system
    Depositor
    Katherine Berry
    Article
    Wang et al RNA. 2021 Apr;27(4):513-526. doi: 10.126
    Insert
    MS2 coat protein
    Tags
    λCI
    Expression
    Bacterial
    Mutation
    V30I, A81G, positions 68-80 deleted (VATQTVGGVELP…
    Promoter
    Synthetic Constitutive Promoter
    Available Since
    May 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    174786-plasmid-map-sequence-id-361842

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Showing: 7021 - 7040 of 7730 results