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IRES-GFP FoxG1

Plasmid

#14935

Depositor

Joan Massague

Article

Seoane et al Cell. 2004 Apr 16. 117(2):211-23.

Insert

FoxG1 (FOXG1 Human)

Tags

IRES-GFP

Expression

Mammalian

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

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pSL3936 (pDonor(AAVS1)_Pse)

Plasmid

#200903

Purpose

Encodes a mini-transposon derived from Pseudoalteromonas Tn7016 with a SA-T2A-PuroR-T2A-eGFP cargo. Total transposon size = 2,024 bp.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

Mini Tn7016 (AAVS1 NGS)

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pSL3936 (pDonor(AAVS1)_Pse)

Plasmid

#200903

Purpose

Encodes a mini-transposon derived from Pseudoalteromonas Tn7016 with a SA-T2A-PuroR-T2A-eGFP cargo. Total transposon size = 2,024 bp.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

Mini Tn7016 (AAVS1 NGS)

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-p58

Plasmid

#166942

Purpose

mammalian expression of p58 (ie ERGIC-53; LMAN1) tagged with GFP

Depositor

Jennifer Lippincott-Schwartz

Article

Ward et al J Cell Biol. 2001 Nov 12;155(4):557-70.

Insert

p58 (Lman1 Rat)

Tags

GFP and chicken lysozyme signal peptide

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-p58

Plasmid

#166942

Purpose

mammalian expression of p58 (ie ERGIC-53; LMAN1) tagged with GFP

Depositor

Jennifer Lippincott-Schwartz

Article

Ward et al J Cell Biol. 2001 Nov 12;155(4):557-70.

Insert

p58 (Lman1 Rat)

Tags

GFP and chicken lysozyme signal peptide

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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IRE1 alpha KA-pcDNA3.EGFP

Plasmid

#13010

Depositor

Fumihiko Urano

Article

Lipson et al Cell Metab. 2006 Sep . 4(3):245-54.

Insert

Inositol Requiring Enzyme-1 (ERN1 Human)

Expression

Mammalian

Mutation

R4L, K599A

Promoter

CMV

Available Since

Nov. 27, 2006

Availability

Academic Institutions and Nonprofits only

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IRE1 alpha KA-pcDNA3.EGFP

Plasmid

#13010

Depositor

Fumihiko Urano

Article

Lipson et al Cell Metab. 2006 Sep . 4(3):245-54.

Insert

Inositol Requiring Enzyme-1 (ERN1 Human)

Expression

Mammalian

Mutation

R4L, K599A

Promoter

CMV

Available Since

Nov. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pBsVchCAST-pyrE

Plasmid

#203813

Purpose

Expression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coli

Depositor

Sheng Yang

Article

Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2

Insert

VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(pyrE)

Use

CRISPR

Expression

Bacterial

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pBsVchCAST-pyrE

Plasmid

#203813

Purpose

Expression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coli

Depositor

Sheng Yang

Article

Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2

Insert

VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(pyrE)

Use

CRISPR

Expression

Bacterial

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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delCMV-mCherry-2x-Rhotekin-GBD

Plasmid

#214142

Purpose

Rho activity sensor

Depositor

Leif Dehmelt

Article

Nanda et al Nat Commun. 2023 Dec 15;14(1):8356. doi:

Insert

3xRhotekin-GBD (Rtkn Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

Only the GTPase binding domain (GBD) is included

Promoter

delCMV

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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delCMV-mCherry-2x-Rhotekin-GBD

Plasmid

#214142

Purpose

Rho activity sensor

Depositor

Leif Dehmelt

Article

Nanda et al Nat Commun. 2023 Dec 15;14(1):8356. doi:

Insert

3xRhotekin-GBD (Rtkn Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

Only the GTPase binding domain (GBD) is included

Promoter

delCMV

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCI-Neo-PTPRT (WT)

Plasmid

#16630

Depositor

Bert Vogelstein

Article

Wang et al Science. 2004 May 21. 304(5674):1164-6.

Insert

PTPRT (PTPRT Human)

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pCI-Neo-PTPRT (WT)

Plasmid

#16630

Depositor

Bert Vogelstein

Article

Wang et al Science. 2004 May 21. 304(5674):1164-6.

Insert

PTPRT (PTPRT Human)

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pRK5-myc-Miro1 P13V

Plasmid

#47889

Purpose

Expresses myc tagged Miro1 P13V constitutively active mutant

Depositor

Pontus Aspenström

Article

Fransson et al J Biol Chem. 2003 Feb 21;278(8):6495-502

Insert

Miro1 P13V (RHOT1 Human)

Tags

myc

Expression

Mammalian

Mutation

P13V, constitutively active mutant

Promoter

CMV

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-myc-Miro1 P13V

Plasmid

#47889

Purpose

Expresses myc tagged Miro1 P13V constitutively active mutant

Depositor

Pontus Aspenström

Article

Fransson et al J Biol Chem. 2003 Feb 21;278(8):6495-502

Insert

Miro1 P13V (RHOT1 Human)

Tags

myc

Expression

Mammalian

Mutation

P13V, constitutively active mutant

Promoter

CMV

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only
HA-MBP-TEVpCS-ADGRG4 CTFdeltaTA-FLAG

Plasmid

#217735

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRG4 (ADGRG4 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-2727

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRG4 CTFdeltaTA-FLAG

Plasmid

#217735

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRG4 (ADGRG4 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-2727

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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Flag p21 T145A

Plasmid

#16241

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2001 Mar . 3(3):245-52.

Insert

p21 (CDKN1A Human)

Tags

Flag

Expression

Mammalian

Mutation

T145A

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
Flag p21 T145A

Plasmid

#16241

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2001 Mar . 3(3):245-52.

Insert

p21 (CDKN1A Human)

Tags

Flag

Expression

Mammalian

Mutation

T145A

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
GFP-Miro1 S156E

Plasmid

#127589

Purpose

Phospho-mimetic of S156

Depositor

Josef Kittler

Article

Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

Insert

Miro1 (RHOT1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

S156E

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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