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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,947 results for: emb

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Showing: 7061 - 7080 of 11947 results

  2. phNFL-P440L

    Plasmid
    #132596
    Purpose
    Expresses human NFL containing the Charcot-Marie-Tooth disease type 2E P440L mutation
    Depositor
    Anthony Brown
    Publication
    Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NEFL (NEFL Human)
    Expression
    Mammalian
    Mutation
    1319C>T; Pro440Leu
    Promoter
    CMV
    Available since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    132596-plasmid-map-sequence-id-260966

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  8. pD10AH840AhCas9 (GB1041)

    Plasmid
    #68207
    Purpose
    Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part
    Depositor
    Diego Orzaez
    Publication
    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.
    Insert
    Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
    Use
    CRISPR and Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed; human codon optimis…
    Available since
    March 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    68207-plasmid-map-sequence-id-135575

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  10. pCMV-myc-mIFITM3-Y20A

    Plasmid
    #58460
    Purpose
    Expresses murine IFITM3-Y20A with an N-terminal myc tag in mammalian cells.
    Depositor
    Jacob Yount
    Publication
    Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    IFITM3 (Ifitm3 Mouse)
    Tags
    myc
    Expression
    Mammalian
    Mutation
    Tyrosine 20 to alanine
    Promoter
    CMV IE
    Available since
    Sept. 10, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. pCVL.SSA TLR (Ani)

    Plasmid
    #45577
    Purpose
    Codes for the SSA-TLR with the target site for I-Ani I in a lentiviral backbone
    Depositor
    Andrew Scharenberg
    Publication
    Kuhar et al Nucleic Acids Res. 2013 Oct 10.
    Inserts
    5' iRFP arm
    eGFP with I-Ani I TS
    +3 mCherry
    3' iRFP arm
    Use
    Lentiviral
    Expression
    Bacterial and Mammalian
    Mutation
    embedded I-Ani I TS from 163-185bp, truncated 25 …
    Promoter
    SFFV
    Available since
    Nov. 4, 2013
    Availability
    Academic Institutions and Nonprofits only
    45577-plasmid-map-sequence-id-67848

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  15. pSB-CMV-MCS-Puro mCherry-Sec 61B -P2A-EGFP-LMNB1

    Plasmid
    #250911
    Purpose
    Dual color expression of mCherry tagged human Sec 61B and EGFP tagged zebrafish LaminB1 in mammalian cell lines
    Depositor
    Philipp Niethammer
    Publication
    Shen et al Nat Cell Biol. 2025 Dec 9. doi: 10.1038/
    Insert
    mCherry-Sec61B-P2A-EGFP-LMNB1 (SEC61B, lmnb1 Zebrafish, Human)
    Tags
    EGFP on LMNB1 and mCherry on Sec61b
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    250911-plasmid-map-sequence-id-501821

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  19. pcDNA3.1-myc:Skp1(dC111):gs6:TEVs:gs6:fLuc:HA

    Plasmid
    #228530
    Purpose
    Firefly luciferase in fusion with a fragment of human Skp1 protein through a flexible linker and a TEV viral protease cleavage site. Expression of fLuc protein is deinhibited by TEV site cleavage.
    Depositor
    Roman Jerala
    Publication
    Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80
    Insert
    myc:Skp1(dC111):gs6:TEVs:gs6:fLuc:HA (SKP1 Photinus pyralis, Synthetic, Human)
    Tags
    HA and myc
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    228530-plasmid-map-sequence-id-456690

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  20. mEosEM-E_Lamin A

    Plasmid
    #226338
    Purpose
    Photoswitchable fluorescent protein for correlative light-electron microscopy; nuclear envelope-targeted mEosEM-E for mammalian expression
    Depositor
    Pingyong Xu
    Publication
    Peng et al Cells. 2022 Mar 23;11(7):1077. doi: 10.3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lamin A (LMNA Human)
    Tags
    mEosEM-E
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    226338-plasmid-map-sequence-id-456227

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Showing: 7061 - 7080 of 11947 results