We narrowed to 13,832 results for: sequence

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pCMV HDM2(NES-)

Plasmid

#12087

Depositor

Tyler Jacks

Article

Boyd et al Nat Cell Biol. 2000 Sep . 2(9):563-8.

Insert

HDM2 (MDM2 Human)

Expression

Mammalian

Mutation

Nuclear-export sequence mutant (NES-): L205A, I20…

Available Since

June 8, 2006

Availability

Academic Institutions and Nonprofits only
pKar2-msGFP-HDEL

Plasmid

#117668

Purpose

Localisation of Endoplasmic Reticulum

Depositor

Pau Ferrer, Benjamin Glick

Article

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

pKar2-msGFP-HDEL

Expression

Yeast

Mutation

Contains the Kar2 promoter and signal sequence fr…

Promoter

pKar2

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pHH29_32 series plasmid

Plasmid

#120901

Purpose

Also known as pHH29_32_HG27, one of the pHH29_32 series plasmids. The component plasmid of a unregulated or a regulated device

Depositor

Domitilla Del Vecchio

Article

Huang et al Nat Commun. 2018 Dec 21;9(1):5415. doi:

Inserts

Ec-TTL-P109
sRNA-A targeting sequence
RBS TIR=974 of GFPop1 gene
GFPop1
RBS TIR=6474 of ECF32mut gene
ECF32mut
T7 terminator
resource competitor

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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CaYin1 NES

Plasmid

#33318

Depositor

Robert Campbell

Article

Ding et al Anal Chem. 2011 Dec 15;83(24):9687-93. d

Insert

CaYin1 NES

Tags

NES sequence LALKLAGLDIGS

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pCHNFI-B2

Plasmid

#31405

Depositor

Richard Gronostajski

Article

Chaudhry et al J Biol Chem. 1998 Jul 17. 273(29):18538-

Insert

Nuclear Factor IB isoform 2 (Nfib Mouse)

Tags

HA

Expression

Mammalian

Mutation

Not the same as reference seq. See sequence for m…

Available Since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only

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pMDR1-221

Plasmid

#69481

Purpose

Deletion Construct

Depositor

Kathleen Scotto

Article

Jin et al Mol Cell Biol. 1998 Jul;18(7):4377-84.

Insert

MDR1 promoter (ABCB1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Insert includes MDR1 promoter sequence from -221 …

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only
pENTR1A_no_ccDB_mUty

Plasmid

#158570

Purpose

V5-tagged sequence of mUty cloned into entry vector for recombination

Depositor

Christian Deschepper

Article

Deschepper et al Sci Rep. 2020 Sep 10;10(1):14900. doi: 1

Insert

Mus musculus ubiquitously transcribed tetratricopeptide repeat containing, Y-linked (Uty), transcript variant 2 (Uty Mouse)

Use

Lentiviral

Tags

V5

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pET21c-IF3mt

Plasmid

#31078

Depositor

Linda Spremulli

Article

Koc et al J Biol Chem. 2002 Sep 20. 277(38):35541-

Insert

Mature form human mitochondrial translational initiation factor 3 (MTIF3 Human)

Tags

His

Expression

Bacterial

Mutation

Includes coding sequence for the mature form. Ami…

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
pJEC581

Plasmid

#174369

Purpose

RFP reporter used to evaluate CRISPRa in bacteria, contains gRNA target sites every 10 bp upstream of the promoter.

Depositor

James Chappell

Article

Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

Insert

RFP with PAM rich sequence upstream promoter

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23117

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-AktAR

Plasmid

#123356

Purpose

FRET-based biosensor for monitoring Akt activity in lipid raft plasma membrane regions.

Depositor

Jin Zhang

Article

Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.

Insert

Lyn-AktAR

Tags

Cerulean, N-terminal targeting sequence from Lyn …

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19, containing H2-Kd Balb/c WK10

Plasmid

#102642

Purpose

Encodes sequence of the murine H2-Kd allele

Depositor

Sai Reddy

Article

Kelton et al Sci Rep. 2017 Apr 4;7:45775. doi: 10.103

Insert

H2-Kd Balb/c

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGIGYF_AH

Plasmid

#148918

Purpose

Insect Expression of DmGIGYF

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF (Gyf Fly)

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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Rap-MESA-20-M-Gal4

Plasmid

#84554

Purpose

MESA target chain with rapamycin FRB ectodomain, 20 extracellular linkers, M cleavage sequence, and Gal4 transactivator

Depositor

Joshua Leonard

Article

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

Rap-MESA-FRB-20-M-Gal4

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pEBB-BT

Plasmid

#36295

Depositor

Ezra Burstein

Article

Maine et al Nat Protoc. 2010 Aug;5(8):1447-59. Epub

Type

Empty backbone

Tags

BT-tag (peptide sequence from P.freudenreichii tr…

Expression

Mammalian

Promoter

EF1alpha

Available Since

June 18, 2012

Availability

Academic Institutions and Nonprofits only

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pPotef-LN22*-3xGNLS-cbx

Plasmid

#86780

Purpose

Expresses lambda N protein fused to triple Gfp coupled to an NLS sequence under the control of the constitutively active Potef promotor.

Depositor

Michael Feldbrügge

Article

Pohlmann et al Elife. 2015 May 18;4. doi: 10.7554/eLife

Insert

LN*

Use

Ustilago maydis expression

Tags

Gfp

Expression

Bacterial

Promoter

Potef

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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Lyn-tdnano-HA

Plasmid

#136512

Purpose

Mammalian expression of a tandem dimer of WT SspB (nano), targeted to membrane by Lyn myristoylation sequence, with triple-HA epitope tag

Depositor

Bianxiao Cui

Article

Hope et al J Mol Biol. 2020 Jun 12;432(13):3739-374

Insert

Lyn nano nano 3HA

Expression

Mammalian

Promoter

CMV

Available Since

May 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISP_IS

Plasmid

#120425

Purpose

This plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3 and IS5.

Depositor

Tamás Fehér

Article

Nyerges et al Synth Biol (Oxf). 2019;4(1):ysz008. doi:

Insert

CRISPR spacers targeting IS1, IS5, IS3

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

constitutive

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pAM750

Plasmid

#207889

Purpose

Level 0 for A3 module (5' TCCC-3' AACA)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

dummy 20nt sequence

Use

Synthetic Biology

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAM749

Plasmid

#207888

Purpose

Level 0 for AD2 module (5' TGAC-3' TCCC)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

dummy 20nt sequence

Use

Synthetic Biology

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAM758

Plasmid

#207897

Purpose

Level 0 for C4 module (5' GCAG-3' TCAG)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

dummy 20nt sequence

Use

Synthetic Biology

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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