We narrowed to 66,033 results for: %s
-
Plasmid#164620PurposeExpresses JIP4 in mammalian cells with a GFP tagDepositorAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only
-
pSMAL
Plasmid#161785PurposeGateway destination vector with bidirectional CMV-SFFV promoter for high expression of gene of interest and GFP.DepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterBidirectional minhCMV and SFFVAvailable sinceDec. 30, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-PE2-SpG-P2A-GFP
Plasmid#159980PurposePrime editing in mammalian cellsDepositorInsertPE2-SpG-P2A-GFP
TagsBpSV40 NLSExpressionMammalianPromoterCMVAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
COVID-SARS2 NSP14/10
Plasmid#159613PurposeBacterial co-expression vector fo Covid-SARS2 NSP14 and NSP10DepositorInsertsTagsHis6, TEV cleavage site and noneExpressionBacterialMutationCodon-optimized for E. coli expressionPromoter(Bicistronic) and T7 - LacOAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-Syn-SomaGCaMP6f2
Plasmid#158756PurposeCell body-targeted GCaMP6f, under synapsin promoter: GCaMP6f followed by a linker, and the EE-RR coiled-coil motif (GCaMP6f-27-EE-RR). As bright as conventional GCaMP6f.DepositorInsertSomaGCaMP6f2
UseAAVExpressionMammalianPromotersynapsin promoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
CGBE1-NG (pBM1246)
Plasmid#140256PurposeCMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFPDepositorInserteUNG-BE4max(R33A)_NG-ΔUGI
ExpressionMammalianMutationR33A in rAPOBEC1, NG mutations in SpCas9, D10A in…PromoterCMVAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-CjCas9-eGFP-HIF1a
Plasmid#137929PurposeExpression of cjCas9 with Hif1a targeting sgRNADepositorInsertCjCas9
UseAAV and CRISPRTagsSV40NLS-HA-T2A-EGFPPromoterEF-1alphaAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCold CL7-Cas12a
Plasmid#124891Purposeobtaining full Cas12a RNPDepositorInsertCas12a
ExpressionBacterialAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-ASAP3Kv-WPRE
Plasmid#132330Purposeexpresses ASAP3Kv(soma-located version of ASAP3, ASAP-family genetically encoded voltage indicator) in cre recombinase expressing mouse/cell, gene delivered by pAAV-EF1a vectorDepositorInsertASAP3KV
UseAAV and Cre/LoxExpressionMammalianPromoterEF1aAvailable sinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEX-SomArchon
Plasmid#126943PurposeCre-dependent expression of SomArchon under CAG promoterDepositorInsertSomArchon
UseAAVTagsGFPExpressionMammalianPromoterCAGAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET30a-HS-Tn5
Plasmid#127916PurposeProkaryotic expression of Hyperstable Tn5 (HS-Tn5) for Illumina library preperation. Developed for the large scale ChIP-Seq and ATAC-Seq experiments for the fruitENCODE and Maize Cistrome projects.DepositorPublicationInsertHyperstable Tn5
TagsE coli elongation factor Ts and Mxe intein - Chit…ExpressionBacterialMutationE54K, L372PPromoterT7Available sinceJuly 5, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRC49-U6-DR-crRNA-BsmbI(x2)-6T; EFS-Puro-2A-Fluc-2A-EGFP_NLS-WPRE
Plasmid#123363PurposeLentiviral vector with empty U6 cassette containing LbCpf1 direct repeat and U6 terminator, with constitutive expression of puromycin resistance, Firefly luciferase, and nuclear EGFP.DepositorInsertEGFP-NLS
UseCRISPR, Lentiviral, and LuciferaseExpressionMammalianPromoterEFSAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR-TRE3G-dCas9-10X GCN4-P2A-mCherry
Plasmid#122132PurposeExpress a dCas9, 10X GCN4, and mCherry cassette from the TRE3G promoterDepositorInsertdCas9-10X GCN4-P2A-mCherry
UseCRISPR and LentiviralExpressionMammalianMutationdCas9 D10A & H840APromoterTRE3GAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Flag-GFP11-pDEST-C
Plasmid#118367PurposeBimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible C-terminal vector with BiFC-tag (GFP11-Flag) to screen interaction partners.DepositorTypeEmpty backboneUseDestinationTagsGFP11-FLAGExpressionMammalianAvailable sinceMay 1, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSU-araC-Cas9
Plasmid#120423PurposeMultigene editing in the Escherichia coli genome using the CRISPR-Cas9 systemDepositorInsertCas9
UseCRISPRExpressionBacterialPromoteraraBADAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGTag-TagRFP-SV40
Plasmid#117807PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertTagRFP
UseSynthetic BiologyAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Lenti-TetON-Sox2
Plasmid#110280PurposeDoxycycline-inducible overexpression of Sox2DepositorInsertSRY (sex determining region Y)-box 2 (Sox2 Mouse)
UseLentiviralTagsNo fusion genesExpressionMammalianMutationWild typePromoterTREAvailable sinceOct. 16, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDNA2.0 6H-TEV-KRAS G12V
Plasmid#111850PurposeExpresses activated human KRAS G12V (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.DepositorInsertKRAS G12V (KRAS Human)
Tags6xHis-tag and TEV protease cleavage sequenceExpressionBacterialMutationChanged Glycine 12 to ValinePromoterT5Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSBinit
Plasmid#110100PurposeE.coli entry and expression vector for FX cloning system, N-terminal pelB signal sequence and C-terminal myc and 6xHisTagDepositorTypeEmpty backboneTagsmyc, HisExpressionBacterialAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX459V2.0-SpCas9-HF1
Plasmid#108293PurposepX459 V2.0 (Plasmid #62988) with the N497A, R661A, Q695A, and Q926A mutationsDepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable sinceMay 21, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by