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pCMVtrunc Affimer6-sfCherry2ΔN12

Plasmid

#231551

Purpose

Mammalian expression of Affimer6 fused to N-terminally truncated superfolder Cherry2, for actin filament organization measurements using fluorescence polarization microscopy

Depositor

Manos Mavrakis

Article

Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

Insert

Affimer6

Tags

sfCherry2

Expression

Mammalian

Promoter

CMVtrunc

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pBRY-nuclear mCherry-IRES-PURO

Plasmid

#52409

Purpose

Constitutively expressed mammalian expression vector encoding nuclear mCherry fluorescent reporter. Puromycin resistance.

Depositor

Jacob Hanna

Article

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

nuclear mCherry

Expression

Mammalian

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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CPEB1

Plasmid

#155472

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CPEB1 (CPEB1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1-SAV1

Plasmid

#166476

Purpose

Expresses fusion of mEGFP and SAV1

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, SAV1 (SAV1 Human)

Expression

Mammalian

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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EXOSC10

Plasmid

#155589

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

EXOSC10 (EXOSC10 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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sgTP53(A)

Plasmid

#85559

Purpose

Expresses sgRNA targeting human TP53 around codon F109

Depositor

Luca Grumolato

Article

Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

Insert

tumor protein p53 (TP53 Human)

Expression

Mammalian

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only
pET20b-mPTER

Plasmid

#222189

Purpose

Bacterial expression of mouse PTER protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Tags

6xHis and Strep

Expression

Bacterial

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMCB306-GFP-mito

Plasmid

#229232

Purpose

Expresses GFP localized to mitochondria

Depositor

Suzanne Pfeffer

Article

Bagnoli et al Proc Natl Acad Sci U S A. 2025 Feb 4;122

Insert

EGFP-MAOA (MAOA Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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KIF5A-GFP-SspB

Plasmid

#214401

Purpose

Expresses fusion of Kinesin heavy chain 5A (1-572) with GFP and SspB (micro)

Depositor

Liting Duan

Article

Liu et al Proc Natl Acad Sci U S A. 2024 Apr 2;121

Insert

KIF5A-GFP-SspB (Kif5a Synthetic, Rat)

Tags

KIF5A-GFP-SspB

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pRRL_SRSF2_P95L_mCherry

Plasmid

#84022

Purpose

expression of SRSF2 mutant in mammalian cells

Depositor

Robert Bradley

Article

Kim et al Cancer Cell. 2015 May 11;27(5):617-30. d

Insert

SRSF2 (SRSF2 Human)

Use

Lentiviral

Tags

2TA-mCherry and Flag

Expression

Mammalian

Mutation

P95L

Promoter

PGK

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only
pRRL_SRSF2_P95R_mCherry

Plasmid

#84021

Purpose

expression of SRSF2 mutant in mammalian cells

Depositor

Robert Bradley

Article

Kim et al Cancer Cell. 2015 May 11;27(5):617-30. d

Insert

SRSF2 (SRSF2 Human)

Use

Lentiviral

Tags

2TA-mCherry and Flag

Expression

Mammalian

Mutation

P95R

Promoter

PGK

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only
pAG156 Mito-FAST

Plasmid

#130723

Purpose

Expresses FAST (also called YFAST) fused to a mitochondria targeting sequence (mito) in mammalian cells

Depositor

Arnaud Gautier

Article

Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

Insert

Mito-FAST

Tags

Myc-tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-hPKL WT

Plasmid

#107159

Purpose

For mammalian expression of Flag-hPKL WT

Depositor

Jesse Rinehart

Article

Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

Insert

Flag-hPKL WT (PKLR Human)

Use

Retroviral

Tags

N-term Flag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-2xSTREP-CCND1

Plasmid

#172648

Purpose

Expresses 2xFLAG-2xSTREP-tagged cyclin D1 in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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YA1531: pAAV_CAG-FLEX-QuasAr3

Plasmid

#107701

Purpose

in vivo voltage imaging

Depositor

Adam Cohen

Article

Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

Insert

QuasAr3

Use

AAV

Expression

Mammalian

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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sgTP53(B)

Plasmid

#85560

Purpose

Expresses sgRNA targeting human TP53 around codon F109

Depositor

Luca Grumolato

Article

Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

Insert

tumor protein p53 (TP53 Human)

Expression

Mammalian

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only
INTS6

Plasmid

#155681

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

INTS6 (INTS6 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-RNF168 delta RING

Plasmid

#133982

Purpose

mammalian expression vector of Flag tagged RNF168 where the N-terminus of RNF168 has been deleted (including RING domain)

Depositor

Daniel Durocher

Article

Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

Insert

RNF168 (RNF168 Human)

Tags

Flag

Expression

Mammalian

Mutation

deletion of RING

Promoter

CMV

Available Since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-CMV-BD10-SAS620-3’dCas9-VPR-synpA

Plasmid

#216323

Purpose

Transactivation of murine Myo7b gene via split dCas9-VPR (REVeRT system).

Depositor

Elvir Becirovic

Article

Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

Insert

Split Cas9-VPR + splice acceptor site

Use

AAV

Promoter

CMV

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-3xsgMyo7b-CMV-5’dCas9-SDS-BD10-SV40pA

Plasmid

#216322

Purpose

Transactivation of murine Myo7b gene via split dCas9-VPR (REVeRT system).

Depositor

Elvir Becirovic

Article

Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

Insert

Split Cas9-VPR + splice donor site + gRNAs targeting the murine Myo7b locus for transactivation

Use

AAV

Promoter

CMV

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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