We narrowed to 30,842 results for: Ide

1. pRsetB-RoTq-Off

   Plasmid

   #234466

   Purpose

   Bacterial expression vector for RoTq-Off

   Depositor

   Gary Yellen

   Article

   Rosen et al Proc Natl Acad Sci U S A. 2025 May 13;12

   Insert

   RoTq-Off
   

   Tags

   7xHis

   Expression

   Bacterial

   Available Since

   May 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX6p-1-BCCP-PupE

   Plasmid

   #113403

   Purpose

   Expresses BCCP-PupE in bacteria

   Depositor

   Min Zhuang

   Article

   Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

   Insert

   BCCP-PupE
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Aug. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHH0103_PLK1_Pkinase

   Plasmid

   #109933

   Purpose

   Protein expression and purification of PLK1_Pkinase

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   PLK1_Pkinase
   

   Expression

   Bacterial

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCHW110033

   Plasmid

   #222441

   Purpose

   Level 0, CDS module (AATG-GCTT) containing Cre recombinase.

   Depositor

   Chih-Hang Wu

   Article

   Chiang et al bioRxiv 2024.03.23.586378

   Insert

   Cre recombinase
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCHW110035

   Plasmid

   #222444

   Purpose

   Level 0, N-terminal tag module (CCAT-AATG) containing LoxP-mCherry-stop.

   Depositor

   Chih-Hang Wu

   Article

   Chiang et al bioRxiv 2024.03.23.586378

   Insert

   LoxP-mCherry-stop
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   no

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRGEB33

   Plasmid

   #158151

   Purpose

   Construction of inPTG-Cas9 plasmids expressing gRNA within an engineered intron for binary vector plant genome editing.

   Depositor

   Kabin Xie

   Article

   Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPR-S12

   Plasmid

   #84031

   Purpose

   To episomally express codon optimized Cas9 and chimeric guide RNA

   Depositor

   Sen Wu

   Article

   Li et al Protein Cell. 2016 Sep;7(9):689-91. doi:

   Inserts

   hSpCas9
   eGFP
   

   Tags

   3x FLAG

   Expression

   Mammalian

   Promoter

   CMV and CMV (downstream of F2A self-cleaving pept…

   Available Since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-IL2sp-6His-Asprosin plasmid

   Plasmid

   #190683

   Purpose

   Expresses His-tagged asprosin in mammalian cells. An IL2 signal peptide at N-terminus allows secretion of asprosin from the transduced cells.

   Depositor

   Atul Chopra

   Article

   Mishra et al STAR Protoc. 2022 Dec 16;3(4):101762. do

   Insert

   IL2-6xHis-Asprosin
   

   Tags

   IL2 signal peptide, 6xHis

   Expression

   Mammalian

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. p32Lb

   Plasmid

   #158154

   Purpose

   Construct conventional Cpf1 vectors that uses U3p and UBI10p to express single crRNA (or PTC/CA) and FnCpf1, respectively. For plant genome editing.

   Depositor

   Kabin Xie

   Article

   Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. TS246_pNKI-2-13_HALO-DPN7

    Plasmid

    #200720

    Purpose

    producing Methyl Tracer HALO protein in bacteria

    Depositor

    Bas van Steensel

    Article

    van Schaik et al Methods Mol Biol. 2022;2458:215-229. doi

    Insert

    HALO-DPN7
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    codon optimized

    Available Since

    May 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. M-NM-sgRNA

    Plasmid

    #48673

    Purpose

    Mammalian U6-driven sgRNA (NMm1) targeting GTCCCCTCCACCCCACAGTG

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with N. meningitidis Cas9, hU6 promoter
    

    Use

    CRISPR

    Promoter

    hU6

    Available Since

    Oct. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-Ec.coaE (pESC124)

    Plasmid

    #50390

    Purpose

    Heterologous expression of E. coli CoA biosynthetic enzyme, DPCK (dephospho-coenzyme A kinase). Contain 6xHis-tag for nickel affinity purification.

    Depositor

    Tadhg Begley, Erick Strauss

    Article

    Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

    Insert

    DPCK
    

    Tags

    6XHIS tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRRL-GFPAdGO-PGK-Neo

    Plasmid

    #136898

    Purpose

    Lentivirus for base editing activatable GFP expression in mammalian cells

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    GFPAdGO
    

    Use

    Lentiviral

    Tags

    2xNLS

    Mutation

    TGG>TGA

    Promoter

    SFFV

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSQT817

    Plasmid

    #53373

    Purpose

    wildtype Cas9 expression plasmid

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV_hDlx_DIO_ChR2_mCherry

    Plasmid

    #224459

    Purpose

    An AAV vector expressing ChR2-mCherry that is dependent on both Cre and the hDlx enhancer

    Depositor

    Gordon Fishell

    Article

    Wu et al bioRxiv 2024.07.20.604399

    Insert

    ChR2-mCherry
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSEED-mCherry

    Plasmid

    #219657

    Purpose

    Permits GoldenGate (BsmBI) assembly of donor plasmids to knock-in mCherry using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    sfGFP SEED Cassette
    

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAV0679

    Plasmid

    #149689

    Purpose

    Bacterial expression of SENP_EuH protease

    Depositor

    Dirk Görlich

    Article

    Vera Rodriguez et al J Cell Biol. 2019 Jun 3;218(6):2006-2020

    Insert

    SENP_EuH protease
    

    Tags

    His14-tag and Tev protease cleavage site

    Expression

    Bacterial

    Available Since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET19b+H3

    Plasmid

    #157807

    Purpose

    Expression of cysteine-free human histone H3 (c111A)

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    H3
    

    Expression

    Bacterial

    Mutation

    C111A

    Promoter

    T7

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHEA

    Plasmid

    #168297

    Purpose

    Bacterial surface display of polypeptides (nanobodies) in E. coli

    Depositor

    Luis Ángel Fernández

    Article

    Marin et al J Bacteriol. 2010 Nov;192(21):5588-602.

    Insert

    Enterohemorraghic E. coli autotransporter EhaA C-terminal fragment (C-EhaA)
    

    Tags

    6xHis, E-tag, and PelB signal peptide

    Expression

    Bacterial

    Promoter

    LacI-Plac

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. His-tagged FL Rec8

    Plasmid

    #45243

    Purpose

    raise antibody against Rec8

    Depositor

    Michael Lampson

    Article

    Chiang et al Curr Biol. 2010 Sep 14;20(17):1522-8. do

    Insert

    Rec8 (Rec8 Mouse)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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