We narrowed to 10,221 results for: gnas
-
Plasmid#214280PurposeExpress split-Rac1 fragments that are fused with CIDs and FPsDepositorInsertsTagsmCerulean (on insert 1) and mVenus (on insert 2)ExpressionMammalianMutationRac1-Q61LPromoterCMVAvailable SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only
-
pIRES-mCerulean-FRB-Rac1/N12-Rac1/13C(T17N)-FKBP-mVenus-CAAX
Plasmid#214281PurposeExpress split-Rac1 fragments that are fused with CIDs and FPsDepositorInsertsTagsmCerulean (N terminal on insert 1) and mVenus (on…ExpressionMammalianMutationRac1-T17NPromoterCMVAvailable SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRES-mCerulean-FRB-RhoA/N12-RhoA/13C(T19N)-FKBP-mVenus-CAAX
Plasmid#214283PurposeExpress split-RhoA fragments that are fused with CIDs and FPsDepositorInsertsTagsmCerulean (N terminal on insert 1) and mVenus (on…ExpressionMammalianMutationRhoA-T19NPromoterCMVAvailable SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTL
Plasmid#203176PurposeCentromeric yeast expression vector, leucine selectionDepositorTypeEmpty backboneExpressionYeastAvailable SinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-2P-N317P
Plasmid#196378PurposeMammalian cell expression of SARS-CoV-2 Spike protein with mutation N317P with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987DepositorInsertS-GSAS-2P-N317P (S Severe acute respiratory syndrome coronavirus 2)
TagsHRV 3C cleavage site (before tags) C terminal on …ExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…Available SinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSems-leader-SNAPf-mEGFPm-IFNAR1(28-557)
Plasmid#192704PurposeExpression of SNAPf and mEGFPm-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopyDepositorInsertIFNAR1 (IFNAR1 Human)
TagsIg k-chain leader sequence, SNAPf-tag, and meGFPm…ExpressionMammalianMutationmeGFPm: gluatmic acid 142 to asparagine and histi…PromoterCMVAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSems-leader-SNAPf-mXFPm-IFNAR1(28-557)
Plasmid#192784PurposeExpression of SNAPf and mXFPm-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopyDepositorInsertIFNAR1 (IFNAR1 Human)
TagsIg k-chain leader sequence, SNAPf-tag, and mXFPmExpressionMammalianMutationmXFPm: tryptophan 66 to phenylalanine, gluatmic a…PromoterCMVAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-PDGFRA-D842V_EGFP-PEST_reporter
Plasmid#172598PurposeMammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-D842V mutant signaling.DepositorInsertmyc-PDGFRA-D842V (PDGFRA Human, Synthetic)
UseEgfp-pest_reporterTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPDGFRA-D842VPromoterCMVAvailable SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-PDGFRA-D842V+R914W_EGFP-PEST_reporter
Plasmid#172599PurposeMammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-D842V+R914W mutant signaling.DepositorInsertmyc-PDGFRA-D842V+R914W (PDGFRA Human, Synthetic)
UseEgfp-pest_reporterTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPDGFRA-D842V+R914WPromoterCMVAvailable SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-PDGFRA-K627R+R914W_EGFP-PEST_reporter
Plasmid#172600PurposeMammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-K627R+R914W mutant signaling.DepositorInsertmyc-PDGFRA-K627R+R914W (PDGFRA Human, Synthetic)
UseEgfp-pest_reporterTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPDGFRA-K627R+R914WPromoterCMVAvailable SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-Myr-DnaJB1(H33Q)-PKAcat-mCherry
Plasmid#181855PurposemCherry-tagged DnaJB1-PKA catalytic subunit fusion N-terminal myristoylation site and H33Q DnaJB1 mutation to block Hsp70 binding; for mammalian expression.DepositorTagsmCherryExpressionMammalianMutationHistidine 33 in DnaJB1 changed to glutamine.PromoterCMVAvailable SinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-DnaJB1(H33Q)-PKAcat-mCherry
Plasmid#181854PurposemCherry-tagged DnaJB1-PKA catalytic subunit fusion with H33Q DnaJB1 mutation to block Hsp70 binding; for mammalian expression.DepositorTagsmCherryExpressionMammalianMutationHistidine 33 in DnaJB1 changed to glutamine.PromoterCMVAvailable SinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
ST-2xTstem(5A)-GFP
Plasmid#162504PurposeExpresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. One wildtype Tac stem region is inserted within ST and the other mutated Tac stem region is inserted between ST and GFP.DepositorTagsGFPExpressionMammalianMutationOne Tac stem region is inserted within ST6Gal1 an…Available SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-Cstem(7A)-Tac(5A)
Plasmid#162499PurposeExpresses GFP tagged Tac mutant in mammalian cells. The five O-glycosylation sites in the stem region are mutated, and the stem region of CD8a with mutations is inserted between GFP and Tac(5A).DepositorInsertTagsGFPExpressionMammalianMutationThe five theronine and two serine residues are mu…Available SinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
JDW 771 (pTol2-kdrl:EYFP-hsKRAS4B-WT-ac/Y)
Plasmid#156413Purposekdrl promoter driving EYFP fused to human KRAS4B with an alpha-crystallin zsYellow reporter flanked by Tol2 sitesDepositorAvailable SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
JDW 832 (pDONR221-V5-mTagBFP2-KRAS4A-G12D)
Plasmid#156404PurposeGateway middle entry clone encoding V5-tagged mTagBFP2-fused to human KRAS4A with G12D mutation, includes a stop codonDepositorInserthuman mutant KRAS4A-G12D (KRAS Human)
UseGateway cloningTagsmTagBFP2Mutationmutant KRAS4A-G12DAvailable SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-RPS14(89-151)
Plasmid#122299Purposeexpresses a part of RPS14 protein with a flag tagDepositorInsertRPS14 (RPS14 Human)
TagsFlagExpressionMammalianMutationdeleted amino acids 1 to 88PromoterCMVAvailable SinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-RPS14(2-88)
Plasmid#122298Purposeexpresses a part of RPS14 protein with a flag tagDepositorInsertRPS14 (partial) (RPS14 Human)
TagsFlagExpressionMammalianMutationdeleted amino acids 89 to 151PromoterCMVAvailable SinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-PRDX5K85R
Plasmid#98694PurposeGateway cloning of human PRDX5-K85RDepositorAvailable SinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-PRDX5K83R
Plasmid#98693PurposeGateway cloning of human PRDX5-K83RDepositorAvailable SinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only