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AP582-2

Plasmid

#70049

Purpose

Expresses Ollas::mCherry::linker::H2B::V5 in bacteria

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

Insert

mCherry::linker::H2B

Tags

Ollas and V5

Expression

Bacterial

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pCY 3010-02

Plasmid

#36210

Depositor

Anne Robinson

Article

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

yeast-enhanced (yE) polylinker

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-Cerulean-hphMX4

Available Since

July 18, 2012

Availability

Academic Institutions and Nonprofits only

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MXS_mCherry

Plasmid

#62408

Purpose

MXS_chaining vector with mCherry

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

mCherry

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_mKO2

Plasmid

#62406

Purpose

MXS_chaining vector with mKO2

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

mKO2

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-pOTC-3H-GFP

Plasmid

#205725

Purpose

Expression of GFP fused with pOTC-3H, a leader peptide from mitochondrial matrix enzyme ornithine transcarbamylase and a thermostable three helix-bundle

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

pOTC-3H-GFP

Expression

Mammalian

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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GeNL(Ca2+)_250/pcDNA3

Plasmid

#85206

Purpose

luminescent reporter for calcium signaling

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

GeNL(Ca2+)_250

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET-tTALE-EmGFP-15b

Plasmid

#138280

Purpose

Express tTALE-EmGFP

Depositor

Kyubong Jo

Article

Shin et al Sci Rep. 2019 Nov 20;9(1):17197. doi: 10

Insert

7-mer tTALE-EmGFP

Use

TALEN

Tags

6x His-tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBORF-DBD

Plasmid

#127463

Purpose

ORF filtering plasmid (lambda cI associated plasmid for pAVA)

Depositor

Joel Malek

Article

Andrews et al J Biol Chem. 2019 Jul 26;294(30):11549-1

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pF hPGK mc2 IRES neo

Plasmid

#206236

Purpose

For expressing cloned exons as circular RNA.

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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MXS_rtTA3

Plasmid

#62428

Purpose

MXS_chaining vector with rtTA3

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Reverse tetracycline transactivator

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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p3s-eCas9-1.1

Plasmid

#104172

Purpose

eCas9-1.1 mammalian expression

Depositor

Jin-Soo Kim

Article

Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

Insert

eCas9-1.1

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

K848A, K1003A, R1060A

Promoter

CMV

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pMK409 (mAID-Nluc BSD)

Plasmid

#121199

Purpose

C-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

mAID-Nluc BSD

Use

CRISPR

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pRSET FlipSuc-90u

Plasmid

#13558

Depositor

Wolf Frommer

Article

Lager et al J Biol Chem. 2006 Oct 13. 281(41):30875-

Insert

FlipThuE W283A

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

W283A in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pET14b_SG

Plasmid

#59737

Purpose

Expresses SH3-GUK domains of human ZO-1

Depositor

Alan Fanning

Article

Lye et al J Biol Chem. 2010 Apr 30;285(18):13907-1

Insert

zonula occludens-1 (TJP1 Human)

Tags

HIS tag and thrombin clvg

Expression

Bacterial

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only
pET-His-Tre

Plasmid

#123129

Purpose

Bacterial expression plasmid for Tre with an N-terminal His-tag.

Depositor

David Liu

Article

Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

Insert

Tre recombinase

Tags

6x His tag

Expression

Bacterial

Promoter

T7

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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epiCRISPRn

Plasmid

#135961

Purpose

Expresses SpCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Xie et al Sci Rep. 2017 May 24;7(1):2320. doi: 10.

Type

Empty backbone

Expression

Mammalian

Available Since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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pDS250

Plasmid

#34969

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2(SS/TM)-GFP-LOVpep

Expression

Yeast

Mutation

V416I

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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MXS_LSS-mKate2

Plasmid

#62402

Purpose

MXS_chaining vector with LSS-mKate2

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

LSS-mKate2

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_mEGFP

Plasmid

#62403

Purpose

MXS_chaining vector with mEGFP

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

mEGFP

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pKB239

Plasmid

#170034

Purpose

Encodes LacI-controlled expression of surface-displayed GNLY and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
Granulysin (GNLY Human, Synthetic, Escherichia coli)

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Promoter

Ptac and PvirK

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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