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pRZ315:CMVp-BD6-AS (50bp)2mm(18-19)-(Canonical V13'SE)-mK2-Ex2-(linker4)-pA

Plasmid

#251324

Purpose

Expression of mK2-Ex2 under CMVp promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Publication

Roas et al Nat Commun. 2026 Jun 16. doi: 10.1038/s4

Insert

mK2-Ex2

Expression

Mammalian

Promoter

CMVp

Available since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pRZ365:CMVe-Spacer(80bp)-TATA-mK2-Ex1-miRS2-mK2-Ex2-miRS1-BSs-pA

Plasmid

#251330

Purpose

Expression of mKate2 under CMVe promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Publication

Roas et al Nat Commun. 2026 Jun 16. doi: 10.1038/s4

Insert

mKate2

Expression

Mammalian

Promoter

CMVe

Available since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pRZ366:CMVp-mK2-Ex1-miRS1-mK2-Ex2-PEST-miRS2-BSs-pA

Plasmid

#251331

Purpose

Expression of mKate2 under CMVp promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Publication

Roas et al Nat Commun. 2026 Jun 16. doi: 10.1038/s4

Insert

mKate2

Expression

Mammalian

Promoter

CMVp

Available since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pCGT001_nuclear-mOrange2

Plasmid

#248083

Purpose

Constitutive expression of NLS-tagged mOrange2 from EF1-alpha promoter (for lentiviral packaging)

Depositor

Arjun Raj

Publication

Van Eyndhoven et al bioRxiv [Preprint]. 2025 Jun 27:2025.06.

Insert

mOrange2

Use

Lentiviral

Tags

SV40 NLS

Expression

Mammalian

Promoter

EF1-alpha

Available since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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CAaNa::GFP

Plasmid

#140507

Purpose

Encodes GFP downstream of an attP/attB flanked Neomycin stop cassette driven by the CAG promoter

Depositor

Mark Emerson

Publication

Schick et al Sci Rep. 2019 Jun 27;9(1):9358. doi: 10.

Insert

attP-Neomycin-PolyA-attB

Use

Chicken expression

Expression

Mammalian

Available since

Nov. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-RFP6 POD-nAb-WPRE

Plasmid

#244974

Purpose

Expresses a fusion protein of a nanobody against RFP and peroxidase (POD) in mammalian cells

Depositor

Hiroyuki Hioki

Publication

Yamauchi et al Commun Biol. 2025 Jun 18;8(1):903. doi:

Insert

RFP6 POD-nAb

Available since

Oct. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-hDPPIII

Plasmid

#233510

Purpose

Mammalian expression vector

Depositor

Shyam Chauhan

Publication

Prajapati et al Biol Chem. 2016 Jun 1;397(6):563-9. doi:

Insert

hDPPIII (DPP3 Human)

Expression

Mammalian

Available since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVAb827

Plasmid

#217500

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb82 backbone, RK2 ori

Depositor

Nico Claassens

Publication

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVAb837

Plasmid

#217501

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb83 backbone, pBBR1 ori

Depositor

Nico Claassens

Publication

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVAb887

Plasmid

#217504

Purpose

pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb88 backbone, pUC ori

Depositor

Nico Claassens

Publication

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

superfolder green fluorescent protein (sfGFP)

Use

Synthetic Biology

Mutation

S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…

Available since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pUC18 3xFLAG COP1

Plasmid

#237334

Purpose

expression in bacterial cells for protein purification

Depositor

Michele Pagano

Publication

Rizzini et al Curr Biol. 2019 Jun 17;29(12):1954-1962.

Insert

COP1 (COP1 Human)

Expression

Bacterial

Available since

May 19, 2025

Availability

Academic Institutions and Nonprofits only

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pUC57 mini-ZsGreen

Plasmid

#222648

Purpose

Vector for cloning gene of interest for in vitro transcription

Depositor

Silvia Monticelli

Publication

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

ZsGreen

Use

In vitro transcription

Mutation

mutated stop codon

Promoter

T7

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-Regnase-1 WT

Plasmid

#222653

Purpose

Mammalian expression vector to express mouse Regnase-1 WT

Depositor

Silvia Monticelli

Publication

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12a (Zc3h12a Mouse)

Expression

Mammalian

Promoter

CMV

Available since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pEC2735 (pSpy0C2)

Plasmid

#220279

Purpose

integrative plasmid for heterologous gene expression in S. pyogenes SF370, integrates into the amyA locus via homologous recombination

Depositor

Emmanuelle Charpentier

Publication

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Type

Empty backbone

Expression

Bacterial

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pEC2796 (pSpy0C4)

Plasmid

#220280

Purpose

integrative plasmid for heterologous gene expression in S. pyogenes SF370, integrates into the sagB locus via homologous recombination

Depositor

Emmanuelle Charpentier

Publication

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Type

Empty backbone

Expression

Bacterial

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pBad33_ISAba30_reRNA

Plasmid

#205323

Purpose

Bacterial expression of ISAba30 TnpB and its reRNA

Depositor

Haoyi Wang

Publication

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISAba30 TnpB

Expression

Bacterial

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pmirGLO-3'UTR mouse Il13

Plasmid

#207125

Purpose

Luciferase vector containing 3'UTR for mouse Il13

Depositor

Silvia Monticelli

Publication

Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

Insert

Interleukin 13 (Il13) 3'UTR (Il13 Mouse)

Use

Luciferase

Expression

Mammalian

Promoter

PGK promoter

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pBad33_ISAam1_reRNA

Plasmid

#205322

Purpose

Bacterial expression of ISAam1 TnpB and its reRNA

Depositor

Haoyi Wang

Publication

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISAam1 TnpB

Expression

Bacterial

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pBad33_ISEc41_reRNA

Plasmid

#205341

Purpose

Bacterial expression of ISEc41 TnpB and its reRNA

Depositor

Haoyi Wang

Publication

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISEc41 TnpB

Expression

Bacterial

Available since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pBad33_ISEc46_reRNA

Plasmid

#205343

Purpose

Bacterial expression of ISEc46 TnpB and its reRNA

Depositor

Haoyi Wang

Publication

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISEc46 TnpB

Expression

Bacterial

Available since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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