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pMaCTag-24

Plasmid

#120003

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with TAP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

TAP

Use

Pcr template

Tags

TAP

Promoter

None

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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Septin 11 isoform II

Plasmid

#120315

Purpose

Septin 11 isoform II mammalian expression

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Expression

Mammalian

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z18

Plasmid

#120061

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mKeima8.5

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mKeima8.5

Use

Pcr template

Tags

mKeima8.5

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z16

Plasmid

#120059

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with Dronpa3

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Dronpa3

Use

Pcr template

Tags

Dronpa3

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z04

Plasmid

#120047

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mTFP1

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mTFP1

Use

Pcr template

Tags

mTFP1

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P20

Plasmid

#120031

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 10xSuntag

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

10xSuntag

Use

Pcr template

Tags

10xSuntag

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P03

Plasmid

#120014

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with moxCerulean3

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

moxCerulean3

Use

Pcr template

Tags

moxCerulean3

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-27

Plasmid

#120006

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with 1xHA

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

1xHA

Use

Pcr template

Tags

1xHA

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-14

Plasmid

#119993

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mNeptune2.5

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mNeptune2.5

Use

Pcr template

Tags

mNeptune2.5

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-10

Plasmid

#119989

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mKOk

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mKOk

Use

Pcr template

Tags

mKOk

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-08

Plasmid

#119987

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mClover3

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mClover3

Use

Pcr template

Tags

mClover3

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-33

Plasmid

#119986

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with FLAG

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

FLAG

Use

Pcr template

Tags

FLAG

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-01

Plasmid

#119980

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with moxBFP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

moxBFP

Use

Pcr template

Tags

moxBFP

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pT2K-CAGGS-U6-sgRNA-M9-IRES-CFP

Plasmid

#114731

Purpose

sgRNA targeting a sequence upstream of the initiator ATG of the cellular Myc gene

Depositor

Martine Roussel

Article

Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

Insert

sgRNA M9

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pLAP-BUBR1-D882N

Plasmid

#114062

Purpose

transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

Depositor

Geert Kops

Article

Suijkerbuijk et al Dev Cell. 2012 Jun 12;22(6):1321-9. doi:

Insert

BUBR1-D882N (BUB1B Human)

Tags

YFP-TEV-S- Human LAP

Expression

Mammalian

Mutation

C2823A and G2826A (siRNA-resistance), BUBR1-D882N

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pLAP-BUBR1-K795R

Plasmid

#114060

Purpose

transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

Depositor

Geert Kops

Article

Suijkerbuijk et al Dev Cell. 2012 Jun 12;22(6):1321-9. doi:

Insert

BUBR1-K795R (BUB1B Human)

Tags

YFP-TEV-S- Human LAP

Expression

Mammalian

Mutation

C2823A and G2826A (siRNA-resistance), BUBR1-K795R

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.3 HA-TSPAN12^11-LEL

Plasmid

#115785

Purpose

Expresses a chimera in mammalian cells: the large extracellular loop of TSPAN12 is replaced with TSPAN11 sequence

Depositor

Harald Junge

Article

Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

Insert

TSPAN12/11 chimera (TSPAN11, TSPAN12 Human)

Tags

HA

Expression

Mammalian

Mutation

large extracellular loop of TSPAN12 replaced with…

Promoter

CMV

Available Since

Oct. 22, 2018

Availability

Academic Institutions and Nonprofits only

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RV-Cag-Dio-mTdt

Plasmid

#87665

Purpose

retroviral vector encoding cre dependent expression of membrane Tdtomato

Depositor

Oscar Marin

Article

Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

Insert

Mem-Tdtomato

Use

Retroviral

Promoter

CAG

Available Since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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RV-Syn-Dio-mTdt

Plasmid

#87667

Purpose

retroviral vector encoding cre dependent expression of membrane Tdtomato

Depositor

Oscar Marin

Article

Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

Insert

Mem-Tdtomato

Use

Retroviral

Tags

palmitylation

Promoter

Synapsin

Available Since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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CSII-IDR2-AGMpygTRIM5-FOS

Plasmid

#79064

Purpose

Express African green monkey pygerythrus TRIM5alpha in mammalan cells

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

TRIM5alpha(African green monkey pygerythrus)-FOS

Tags

OneSTrEP-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

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