We narrowed to 14,375 results for: Cas9

Showing: 7121 - 7140 of 14375 results

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pUFlip-floxed2A-KalTA4-; HE1A:BFP -2

Plasmid

#180012

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-KalTA4; HE1A: BFP

Use

Synthetic Biology

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001147380)

Plasmid

#80225

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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SPHK1 gRNA (BRDN0001147454)

Plasmid

#76813

Purpose

3rd generation lentiviral gRNA plasmid targeting human SPHK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SPHK1 (SPHK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-Puro_siKO-2TO

Plasmid

#86697

Purpose

Tetracycline inducible (2TO version) expression of guide RNA targeted to AAVS1 locus

Depositor

Ludovic Vallier

Article

Bertero et al Development. 2016 Dec 1;143(23):4405-441

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

H1 2TO

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-eNme2-T.2-ABE8e

Plasmid

#185669

Purpose

Expresses eNme2-T.2-ABE8e in mammalian cells

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

ecTadA(8e)-neNme2-T.2

Use

CRISPR

Expression

Mammalian

Mutation

Nme2Cas9 D16A/E47K/R63K/V68M/A116T/T123A/D152N/E1…

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-egfp-U3-Osp.gRNA

Plasmid

#166034

Purpose

Empty backbone for expression of gRNA-expressing integration deficient lentiviral particles.

Depositor

Yujia Cai

Article

Ling et al Nat Biomed Eng. 2021 Jan 4. pii: 10.1038

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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TRIM28 gRNA (BRDN0001162487)

Plasmid

#76139

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRIM28

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRIM28 (TRIM28 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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gh262

Plasmid

#106853

Purpose

expression of gRNA targeting ALG9

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

ALG9 (ALG9 Human)

Expression

Mammalian

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-sgRNA-lib 2.0

Plasmid

#89638

Purpose

sgRNA expression in mammalian cells after Gateway cloning

Depositor

Wensheng Wei

Article

Zhu et al Nat Biotechnol. 2016 Dec;34(12):1279-128

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Feb. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pUS2-SH231

Plasmid

#115150

Purpose

Cas9-GFP, dual gRNA expression vector for targeted integration of repair templates into safe harbor 231 locus in chromosome 4

Depositor

Raymond Monnat, Michael Phelps

Article

Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

Insert

Dual SH231 gRNAs

Use

CRISPR

Tags

3xFLAG-SV40NLS, GFP, and Nucleoplasmin NLS

Expression

Mammalian

Promoter

U6, CBh

Available Since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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TRIB2 gRNA (BRDN0001144754)

Plasmid

#75602

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRIB2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRIB2 (TRIB2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TRIB2 gRNA (BRDN0001148141)

Plasmid

#75603

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRIB2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRIB2 (TRIB2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SPHK1 gRNA (BRDN0001147534)

Plasmid

#76814

Purpose

3rd generation lentiviral gRNA plasmid targeting human SPHK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SPHK1 (SPHK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SPHK2 gRNA (BRDN0001146882)

Plasmid

#77096

Purpose

3rd generation lentiviral gRNA plasmid targeting human SPHK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SPHK2 (SPHK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SPHK1 gRNA (BRDN0001148403)

Plasmid

#76815

Purpose

3rd generation lentiviral gRNA plasmid targeting human SPHK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SPHK1 (SPHK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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BLADE

Plasmid

#134912

Purpose

Empty backbone for cloning sgRNA sequence to be used in nanoblades system

Depositor

Philippe Mangeot, Théophile Ohlmann, Emiliano Ricci

Article

Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Promoter

U6

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pX458_Ruby

Plasmid

#110164

Purpose

Cas9 from S. pyogenes with Ruby2, and cloning backbone for sgRNA

Depositor

Philip Hublitz

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Insert

hSpCas9

Use

CRISPR

Tags

3xFLAG and Ruby2

Expression

Mammalian

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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TRIM28 gRNA (BRDN0001146162)

Plasmid

#76142

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRIM28

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRIM28 (TRIM28 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-mRFP-; HE1A:BFP -0

Plasmid

#180007

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-mRFP; HE1A: BFP

Use

Synthetic Biology

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pYLsgRNA-AtU3d/LacZ

Plasmid

#66201

Purpose

cloning of sgRNAs for expression in plants

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Type

Empty backbone

Use

Cloning vector

Promoter

AtU3d

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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