We narrowed to 73,340 results for: LAS

1. GL4.10 E2E1- Prg4 promoter-luciferase

   Plasmid

   #195035

   Purpose

   Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector

   Depositor

   Cheng-Hai Zhang, Andrew Lassar

   Article

   Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

   Insert

   Prg4 promoter plus Enhancer 1 and Enhancer 2 (PRG4 Bovine)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL4.10 E3E2E1-Prg4 promoter-luciferase

   Plasmid

   #195036

   Purpose

   Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector

   Depositor

   Cheng-Hai Zhang, Andrew Lassar

   Article

   Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

   Insert

   Prg4 promoter plus Enhancer 1, Enhancer 2, and Enhancer 3 (PRG4 Bovine)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGL4.10 E4E3E2E1-Prg4 promoter-luciferase

   Plasmid

   #195037

   Purpose

   Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector

   Depositor

   Cheng-Hai Zhang, Andrew Lassar

   Article

   Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

   Insert

   pGL4.10 E4E3E2E1-Prg4 promoter-luciferase (PRG4 Bovine)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pNKX2-5-GFP-T2A

   Plasmid

   #203608

   Purpose

   Donor plasmid to create 5' knockin of NKX2-5 with GFP-T2A

   Depositor

   Matthew Birket

   Article

   Douglas et al Stem Cell Res. 2023 Dec 3;74:103262. doi

   Insert

   NKX2-5-GFP-T2A-NKX2-5 (NKX2-5 Human)
   

   Use

   Human targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHD22Y/PfPRMT5 deletion

   Plasmid

   #203161

   Purpose

   for disruption of PfPRMT5 gene

   Depositor

   Liwang Cui

   Article

   Lucky et al Commun Biol. 2023 Jun 22;6(1):659. doi:

   Insert

   PfPRMT5 fragment
   

   Use

   Tags

   3 myc tags and 3' UTR of P. berghei dihydrof…

   Expression

   Mammalian

   Mutation

   Promoter

   No

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL4.11_Nued2_E5

   Plasmid

   #203834

   Purpose

   Luciferase reporter plasmid for Igf1 enhancer 5

   Depositor

   Ivo Spiegel

   Article

   Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

   Insert

   E5 (Igf1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTP-PCR C3

   Plasmid

   #189620

   Purpose

   Cloned Reduced Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteria

   Depositor

   Bogumil Karas

   Article

   Cochrane et al Biology (Basel). 2020 Oct 26;9(11). pii:

   Insert

   Reduced Mitochondrial Genome of Thalassiosira pseudonana
   

   Use

   Synthetic Biology; Diatom expression

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Removed repeat region, 10-kbp deletion, and minor…

   Promoter

   Available since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRISPRa_all-in-one_Bip gRNA1

   Plasmid

   #183696

   Purpose

   Expresses gRNA targeting chinise hamster Bip (Hspa5) with CRISPRa components and mCherry, blasticidin selective

   Depositor

   Edward Avezov

   Article

   Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

   Insert

   gRNA targeting chinese hamster Bip (Hspa5)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT7-EGFP-C1-HsAGO2-F2V2_L

   Plasmid

   #146885

   Purpose

   Mammalian Expression of HsAGO2-F2V2

   Depositor

   Elisa Izaurralde

   Article

   Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

   Insert

   HsAGO2-F2V2 (AGO2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   sequence differs in the first 7 AA from the seque…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pT7-EGFP-C1-HsNot1iso2_J

    Plasmid

    #146700

    Purpose

    Mammalian Expression of HsNot1iso2

    Depositor

    Elisa Izaurralde

    Article

    Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

    Insert

    HsNot1iso2 (CNOT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    4 silent and two non silent G917E and R2353Q muta…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. iEscSnFR

    Plasmid

    #182807

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Article

    Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

    Insert

    iEscSnFR
    

    Use

    Tags

    6xHis, HA and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. iFluoxSnFR

    Plasmid

    #182808

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Article

    Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

    Insert

    iFluoxSnFR
    

    Use

    Tags

    6xHis, HA and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3_HLA-cmyc-GRM3 C127A TMD Y616M

    Plasmid

    #140454

    Purpose

    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and Y616M mutant

    Depositor

    Erik Procko

    Insert

    GRM3 (GRM3 Human)
    

    Use

    Tags

    Signal/leader sequence from HLA class I histocomp…

    Expression

    Mammalian

    Mutation

    Cysteine 127 to alanine, tyrosine 616 to methioni…

    Promoter

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3_HLA-cmyc-GRM3 C127A TMD L624N

    Plasmid

    #140455

    Purpose

    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and L624N mutant

    Depositor

    Erik Procko

    Insert

    GRM3 (GRM3 Human)
    

    Use

    Tags

    Signal/leader sequence from HLA class I histocomp…

    Expression

    Mammalian

    Mutation

    Cysteine 127 to alanine, leucine 624 to asparagine

    Promoter

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3_HLA-cmyc-GRM3 C127A TMD A634N

    Plasmid

    #140456

    Purpose

    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and A634N mutant

    Depositor

    Erik Procko

    Insert

    GRM3 (GRM3 Human)
    

    Use

    Tags

    Signal/leader sequence from HLA class I histocomp…

    Expression

    Mammalian

    Mutation

    Cysteine 127 to alanine, alanine 634 to asparagine

    Promoter

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCEP4_HLA-flag-GRM3 C127A-VN

    Plasmid

    #140449

    Purpose

    Mammalian expression plasmid for FLAG-tagged mGluR3 fused to the N-terminal half of split fluorescent Venus (VN)

    Depositor

    Erik Procko

    Insert

    GRM3 (GRM3 Human)
    

    Use

    Tags

    FLAG tag, Signal/leader sequence from HLA class I…

    Expression

    Mammalian

    Mutation

    Cysteine 127 to alanine

    Promoter

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCEP4_HLA-myc-GRM3 C127A-VC

    Plasmid

    #140450

    Purpose

    Mammalian expression plasmid for c-myc-tagged mGluR3 fused to the C-terminal half of split fluorescent Venus (VC)

    Depositor

    Erik Procko

    Insert

    GRM3 (GRM3 Human)
    

    Use

    Tags

    Signal/leader sequence from HLA class I histocomp…

    Expression

    Mammalian

    Mutation

    Cysteine 127 to alanine

    Promoter

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3_HLA-cmyc-GRM3 C127A TMD I578A

    Plasmid

    #140452

    Purpose

    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and I578A mutant

    Depositor

    Erik Procko

    Insert

    GRM3 (GRM3 Human)
    

    Use

    Tags

    Signal/leader sequence from HLA class I histocomp…

    Expression

    Mammalian

    Mutation

    Cysteine 127 to alanine, isoluecine 578 to alanine

    Promoter

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3_HLA-cmyc-GRM3 C127A TMD F588V

    Plasmid

    #140453

    Purpose

    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and F588V mutant

    Depositor

    Erik Procko

    Insert

    GRM3 (GRM3 Human)
    

    Use

    Tags

    Signal/leader sequence from HLA class I histocomp…

    Expression

    Mammalian

    Mutation

    Cysteine 127 to alanine, phenylalanine 588 to val…

    Promoter

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Pk_P41-Cd4-bio-His

    Plasmid

    #126847

    Purpose

    Expression of recombinant P. knowlesi protein in mammalian cells

    Depositor

    Gavin Wright

    Article

    Muller-Sienerth et al Malar J. 2020 Jan 17;19(1):31. doi: 10.1

    Insert

    P41 (PKH_030970 Plasmodium knowlesi)
    

    Use

    Tags

    ratCd4(d3+4)-bio-6His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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