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Bxb1-GT_AIO-TetOn_mNgn2 _Down-Tandem

Plasmid

#229795

Purpose

Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression of mouse neurogenin 2

Depositor

Richard Davis

Article

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Insert

TRE-mNgn2; rtTA-T2A-mTagBFP2 (Neurog2 Mouse)

Use

Synthetic Biology

Tags

NLS

Expression

Mammalian

Promoter

TRE and CAG

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pBK1304-AAV-EFSNC-dCjCas9-KRAB-MECP2

Plasmid

#223149

Purpose

Expression of KRAB and truncated MECP2 with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

KRAB-MECP2 (MECP2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 204-310 (MECP2)

Promoter

EF1a

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST-Myc-FBW7 Delta F-Box

Plasmid

#197452

Purpose

The plasmid expresses F-box deleted version of Myc-tagged FBW7 human isoform 1. Used for mammalian overexpression of FBW7alpha delta-F-Box and detection by western blotting.

Depositor

Omar Khan

Article

Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:

Insert

FBW7 (FBXW7 Human)

Tags

Myc

Expression

Mammalian

Mutation

FBW7 Fbox deleted.

Promoter

CMV

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-BBS3/Arl6 [N302/10R]

Plasmid

#188211

Purpose

Mammalian Expression Plasmid of anti-BBS3/Arl6 (Human). Derived from hybridoma N302/10

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-BBS3/Arl6 (Homo sapiens) recombinant mouse monoclonal antibody (ARL6 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Miro2 [N384/63R]

Plasmid

#182114

Purpose

Mammalian Expression Plasmid of anti-Miro2 (Human). Derived from hybridoma N384/63.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Miro2 (Homo sapiens) recombinant mouse monoclonal antibody (RHOT2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Histone H3.3 [N158/28R]

Plasmid

#182106

Purpose

Mammalian Expression Plasmid of anti-Histone H3.3 (Rat). Derived from hybridoma N158/28.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Histone H3.3 (Rattus norvegicus) recombinant mouse monoclonal antibody (H3f3b Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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GluN2B/NR2B glutamate receptor scFv [N59/20]

Plasmid

#182092

Purpose

Mammalian Expression of GluN2B/NR2B glutamate receptor scFv. Derived from hybridoma N59/20.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting GluN2B/NR2B glutamate receptor (Rattus norvegicus) (Grin2b Mouse)

Tags

Sortase, His

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pOPINB-hRNF216(457-784)

Plasmid

#171922

Purpose

Expression of human RNF216 (Triad3A) codon optimized for E. coli and insect cells. Catalytic helix-RBR-helix construct suitable for activity assays.

Depositor

Bernhard Lechtenberg

Article

Cotton et al Mol Cell. 2022 Feb 3;82(3):598-615.e8. d

Insert

E3 ubiquitin-protein ligase RNF216 (RNF216 Human)

Tags

3C protease cleavage site and 6x-His tag

Expression

Bacterial

Mutation

codon optimised for expression in E. coli

Promoter

T7

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pOPINB-hRNF216(511-784)

Plasmid

#171923

Purpose

Expression of human RNF216 (Triad3A) codon optimized for E. coli and insect cells. Catalytic RBR-helix construct suitable for activity assays.

Depositor

Bernhard Lechtenberg

Article

Cotton et al Mol Cell. 2022 Feb 3;82(3):598-615.e8. d

Insert

E3 ubiquitin-protein ligase RNF216 (RNF216 Human)

Tags

3C protease cleavage site and 6x-His tag

Expression

Bacterial

Mutation

codon optimised for expression in E. coli

Promoter

T7

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Ankyrin-B [N105/17R]

Plasmid

#177494

Purpose

Mammalian Expression Plasmid of anti-Ankyrin-B (Human). Derived from hybridoma N105/17.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Ankyrin-B (Homo sapiens) recombinant mouse monoclonal antibody (ANK2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Ankyrin-G (blotting) [N106/20R]

Plasmid

#177495

Purpose

Mammalian Expression Plasmid of anti-Ankyrin-G (blotting) (Human). Derived from hybridoma N106/20.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Ankyrin-G (blotting) (Homo sapiens) recombinant mouse monoclonal antibody (ANK3 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-TrpV1 [N221/17R]

Plasmid

#177517

Purpose

Mammalian Expression Plasmid of anti-TrpV1 (Rat). Derived from hybridoma N221/17.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-TrpV1 (Rattus norvegicus) recombinant mouse monoclonal antibody (Trpv1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-GluN2C [N422/18R]

Plasmid

#177551

Purpose

Mammalian Expression Plasmid of anti-GluN2C/NR2C glutamate receptor (Rat). Derived from hybridoma N422/18.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GluN2C/NR2C glutamate receptor (Rattus norvegicus) recombinant mouse monoclonal antibody (Grin2c Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-GABA(A)R, Alpha4 [N398A/34R]

Plasmid

#177543

Purpose

Mammalian Expression Plasmid of anti-GABA(A)R, Alpha4 (Rat). Derived from hybridoma N398A/34.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GABA(A)R, Alpha4 (Rattus norvegicus) recombinant mouse monoclonal antibody (Gabra4 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-Beta4-spectrin [N393/2R]

Plasmid

#177541

Purpose

Mammalian Expression Plasmid of anti-Beta4-spectrin (Human). Derived from hybridoma N393/2.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Beta4-spectrin (Homo sapiens) recombinant mouse monoclonal antibody (SPTBN4 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-NrCAM [N343/26R]

Plasmid

#177534

Purpose

Mammalian Expression Plasmid of anti-NrCAM (Mouse). Derived from hybridoma N343/26.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-NrCAM (Mus musculus) recombinant mouse monoclonal antibody (Nrcam Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-Neuroligin-2 [L107/39R]

Plasmid

#177452

Purpose

Mammalian Expression Plasmid of anti-Neuroligin-2 (Mouse). Derived from hybridoma L107/39.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Neuroligin-2 (Mus musculus) recombinant mouse monoclonal antibody (Nlgn2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-Calbindin [L109/57R]

Plasmid

#177455

Purpose

Mammalian Expression Plasmid of anti-Calbindin (Human). Derived from hybridoma L109/57.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Calbindin (Homo sapiens) recombinant mouse monoclonal antibody (CALB1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-CASPR/Neurexin IV [K65/35R]

Plasmid

#177445

Purpose

Mammalian Expression Plasmid of anti-CASPR/Neurexin IV (Rat). Derived from hybridoma K65/35.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-CASPR/Neurexin IV (Rattus norvegicus) recombinant mouse monoclonal antibody (Cntnap1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-GABARAPL1 G116

Plasmid

#123102

Purpose

Expresses 3xFLAG-GABARAPL1 G116 in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Deleted amino acid 117. Stop codon after G116

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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