We narrowed to 16,184 results for: 034

1. pCSC-NGN2-IRES-ISL1-T2A-LHX3

   Plasmid

   #221814

   Purpose

   Lentiviral vector expressing human NEUROG2, ISL1 and LHX3 for inducing pluripotent stem cells into cholinergic motor neurons.

   Depositor

   Baojin Ding

   Article

   Sepehrimanesh et al Am J Physiol Cell Physiol. 2020 Oct 1;31

   Inserts

   NEUROG2 (NEUROG2 Human)
   ISL1 (ISL1 Human)
   LHX3 (LHX3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNJP

   Plasmid

   #115494

   Purpose

   Expression vector for NLS-iRFP-NLS, JNK KTR-mCherry, and mKO-MK2 in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Miura et al Cell Rep. 2018 Sep 4;24(10):2658-2668. d

   Insert

   NLS-iRFP-NLS-P2A-JNK KTR-mCherry-P2A-mKO-MK2 (MAPK14, MAPK8 Synthetic, Human, Mouse)
   

   Use

   Tags

   iRFP, mCherry, and mKO

   Expression

   Mammalian

   Mutation

   mCherry S227F, mCherry G229R, mKO V211G

   Promoter

   CAG

   Available Since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQ-Elo-HB-C-A(1-630)

   Plasmid

   #171086

   Purpose

   Co-expresses of ΔC-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOB

   Depositor

   Joan Conaway

   Article

   He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

   Inserts

   Elongin B (ELOB Human)
   Elongin C (ELOC Human)
   Elongin A (ELOA Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Deleted N-term 26aa and C-term 142aa for mutant e…

   Promoter

   T5 promoter

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQ-Elo-HB-C-A(400-772)

   Plasmid

   #171087

   Purpose

   Co-expresses of ΔN-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOB

   Depositor

   Joan Conaway

   Article

   He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

   Inserts

   Elongin B (ELOB Human)
   Elongin C (ELOC Human)
   Elongin A (ELOA Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Deleted N-term 26+399 amino acids for mutant expr…

   Promoter

   T5 promoter and T7 promoter

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQ-Elo-HB-C-A(1-772)

   Plasmid

   #171085

   Purpose

   Co-expresses of WT-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOB

   Depositor

   Joan Conaway

   Article

   He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

   Inserts

   Elongin B (ELOB Human)
   Elongin C (ELOC Human)
   Elongin A (ELOA Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Deleted N-term 26 amino acids for expression purp…

   Promoter

   T5 promoter

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. GCN5 core-dCas9-CBP core

   Plasmid

   #179555

   Purpose

   encodes S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human CBP core (aa 1084-1701) (CREBBP, KAT2A Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. GCN5 FL-dCas9-CBP core

   Plasmid

   #179556

   Purpose

   encodes S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human CBP core (aa 1084-1701) (CREBBP, KAT2A Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available Since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGBdest-10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1_F32A-I35A-I36A_-GFP-TEV-StrepII

   Plasmid

   #192705

   Purpose

   Plasmid for the Expression of the E3 complex with an FII (F32A, I35A, and I36A) mutation. SMC Internal reference: SMC1728

   Depositor

   Sascha Martens

   Inserts

   ATG5 (ATG5 Human)
   ATG7 (ATG7 Human)
   ATG10 (ATG10 Human)
   ATG12 (ATG12 Human)
   ATG16L1
   

   Use

   Tags

   -GFP-TEV-StrepII and 10x-His-TEV

   Expression

   Insect

   Mutation

   F32A-I35A-I36A

   Promoter

   Available Since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. HisGB1-Fusion + CBP TAZ1(340-439)

   Plasmid

   #173762

   Purpose

   Bacterial coexpression of CBP TAZ1 and CITED2/Hif1a activation domain constructs

   Depositor

   Peter Wright

   Article

   Appling et al Structure. 2021 Sep 11. pii: S0969-2126(

   Inserts

   CITED2(216-246):Hif1a(796-826) (HIF1A, CITED2 Human)
   CBP (340-439) (Crebbp Mouse)
   

   Use

   Tags

   His6GB1 and thrombin

   Expression

   Bacterial

   Mutation

   fusion of CITED2 and Hif1a

   Promoter

   T7

   Available Since

   Feb. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. HisGB1-FusionL21A + CBP TAZ1(340-439)

    Plasmid

    #173763

    Purpose

    Bacterial Coexpression of CBP TAZ1 with CITED2/Hif1a activation domain constructs

    Depositor

    Peter Wright

    Article

    Appling et al Structure. 2021 Sep 11. pii: S0969-2126(

    Inserts

    CITED2(216-246L231A):Hif1a(796-826) (HIF1A, CITED2 Human)
    CBP (340-439) (Crebbp Mouse)
    

    Use

    Tags

    His6GB1 and thrombin

    Expression

    Bacterial

    Mutation

    fusion of CITED2 and Hif1a

    Promoter

    T7

    Available Since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. HIsGB1-FusionL63A + CBP TAZ1(340-439)

    Plasmid

    #173764

    Purpose

    Bacterial coexpression of CBP TAZ1 with CITED2/Hif1a activation domain constructs

    Depositor

    Peter Wright

    Article

    Appling et al Structure. 2021 Sep 11. pii: S0969-2126(

    Inserts

    CITED2(216-246):Hif1a(796-826L822A) (HIF1A, CITED2 Human)
    CBP (340-439) (Crebbp Mouse)
    

    Use

    Tags

    His6GB1 and thrombin

    Expression

    Bacterial

    Mutation

    fusion of CITED2 and Hif1a

    Promoter

    T7

    Available Since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. TRUPATH Triple GaZ

    Plasmid

    #196053

    Purpose

    Encodes a G alpha subunit (GNAZ) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphaz-RLuc8 (GNAZ Human)
    GGamma1-GFP2 (GNGT1 Human)
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))

    Plasmid

    #105842

    Purpose

    Donor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichment

    Depositor

    Michael Ward

    Article

    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

    Inserts

    Neurogenin 2 (NEUROG2 Human)
    ISL LIM Homeobox 1 (ISL1 Human)
    LIM Homeobox 3 (LHX3 Human)
    Optimized mApple
    rtTA3G
    SBP Delta-LNGFR
    

    Use

    CRISPR and TALEN

    Tags

    Expression

    Mutation

    Promoter

    CAG, EF-1alpha, and TRE3G

    Available Since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. TRUPATH Triple Gagust

    Plasmid

    #196054

    Purpose

    Encodes a G alpha subunit (GNAT3) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphaGustducin-RLuc8 (GNAT3 Human)
    GGamma1-GFP2 (GNGT1 Human)
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti-EF1a-HIF2a dPA (P405A, P531A)

    Plasmid

    #232954

    Purpose

    Stably express a constitutively stable version of HIF2a (HIF2a dPA)

    Depositor

    William Kaelin

    Article

    Jiang et al Cell. 2025 Feb 25:S0092-8674(25)00156-4.

    Insert

    HIF2a (EPAS1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    P405A, P531A

    Promoter

    EF1a

    Available Since

    March 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIRES-mCerulean-GAI1-92-KRas/N12-KRas/13C(Q61L)-GID1-mVenus-CAAX

    Plasmid

    #232262

    Purpose

    Express split-KRas fragments that are fused with CIDs and FPs

    Depositor

    Cliff Stains

    Article

    He et al ACS Chem Biol. 2025 Aug 26. doi: 10.1021

    Inserts

    mCerulean-GAI1-92-KRas/N12 (KRAS Human)
    KRas/13C(Q61L)-GID1-mVenus-CAAX (KRAS Human)
    

    Use

    Tags

    mCerulean and mVenus

    Expression

    Mammalian

    Mutation

    KRas-Q61L

    Promoter

    CMV

    Available Since

    Sept. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIRES-mCerulean-GAI1-92-KRas/N12-KRas/13C(S17N)-GID1-mVenus-CAAX

    Plasmid

    #232263

    Purpose

    Express split-KRas fragments that are fused with CIDs and FPs

    Depositor

    Cliff Stains

    Article

    He et al ACS Chem Biol. 2025 Aug 26. doi: 10.1021

    Inserts

    mCerulean-GAI1-92-KRas/N12 (KRAS Human)
    KRas/13C(S17N)-GID1-mVenus-CAAX (KRAS Human)
    

    Use

    Tags

    mCerulean and mVenus

    Expression

    Mammalian

    Mutation

    KRas-S17N

    Promoter

    CMV

    Available Since

    Sept. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBIG1a-PAF1CdeltaWDR61

    Plasmid

    #231745

    Purpose

    Protein expression in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Inserts

    CTR9 (CTR9 Human)
    CDC73 (CDC73 Human)
    PAF1 (PAF1 Human)
    LEO1 (LEO1 Human)
    

    Use

    Tags

    3C-Strep(II) and Flag-3C

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUC18_BCR_GUSB_ABL1_e14a3

    Plasmid

    #190885

    Purpose

    molecular monitoring of atypical BCR::ABL1 fusion transcripts

    Depositor

    Nicholas Cross

    Article

    Schafer et al J Cancer Res Clin Oncol. 2021 Oct;147(10

    Inserts

    e14a3 BCR::ABL1 fusion transcript
    ABL1 cDNA (ABL1 Human)
    GUSB cDNA (GUSB Human)
    BCR (BCR Human)
    

    Use

    Cloned cdna transcripts for use in rt-qpcr bcr::a…

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FoxO6deltaCt

    Plasmid

    #124486

    Purpose

    Expresses dominant negative form of mouse FoxO6 with deleted C-term transactivation domain. T26A/S184A mutations increase nuclear localization of FoxO6.

    Depositor

    Anne Brunet

    Article

    Salih et al Genes Dev. 2012 Dec 15;26(24):2780-801.

    Insert

    FoxO6 (Foxo1, Foxo4, Foxo3, Foxo6 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    T26A, S184A, transactivation domain deleted from …

    Promoter

    Available Since

    June 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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