We narrowed to 2,475 results for: T2A

1. CMV-H2B-sfGFP-T2A-iCre

   Plasmid

   #183351

   Purpose

   Histone H2B-sfGFP and iCre recombinase

   Depositor

   Mario Capecchi

   Insert

   Histone H2B-sfGFP-T2A-iCre
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Available Since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDH-EF1a-tetR-T2A-iRFP

   Plasmid

   #176028

   Purpose

   construct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome iRFP720

   Depositor

   Irmela Jeremias

   Article

   Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

   Inserts

   tetR
   iRFP
   

   Use

   Lentiviral

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPaint-T2A-Nluc-3xP3-RFP

   Plasmid

   #127563

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Nluc-SV40 and 3xP3-RFP visible eye marker.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   Nluc
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   Aug. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCXLE-dCas9VPPH-T2A-GFP-shP53

   Plasmid

   #102897

   Purpose

   EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-P300core-p65-HSF1 followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   dCas9VPPH-T2A-GFP-shP53
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   CAG

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS-mTagBFP2-T2A-iCreERT2 (PAGSAS)

   Plasmid

   #137864

   Purpose

   Monomeric TagBFP2 and iCre-ERT2 recombinase (PAGSAS N-terminus)

   Depositor

   Mario Capecchi

   Insert

   mTagBFP2-T2A-iCreERT2
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Available Since

   March 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-Cas12i-Max-T2A-EGFP

   Plasmid

   #188268

   Purpose

   Mammalian expression, Genome editing

   Depositor

   Wei Li

   Article

   Chen et al Innovation (Camb). 2022 May 26;3(4):1002

   Insert

   Cas12i-Max
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CAG

   Available Since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-Cas12i-HiFi-T2A-EGFP

   Plasmid

   #188269

   Purpose

   Mammalian expression, Genome editing

   Depositor

   Wei Li

   Article

   Chen et al Innovation (Camb). 2022 May 26;3(4):1002

   Insert

   Cas12i-HiFi
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CAG

   Available Since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-His6-Sumo-IntNtr-eSrt2A

   Plasmid

   #126015

   Purpose

   Bacterial expression of IntNtr-eSrt2A fusion protein (with N-terminal His6-Sumo)

   Depositor

   Thomas Muir

   Insert

   His6-Sumo-IntNtr-eSrt2A
   

   Tags

   6xHis-SUMO

   Expression

   Bacterial

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT2ADW-lox-mCherryNLS-pA-lox-4493NES

   Plasmid

   #138374

   Purpose

   Booster-PKA for the generation of mice

   Depositor

   Michiyuki Matsuda

   Article

   Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

   Insert

   lox-mCherry-lox-Booster-PKA
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEMT-PT2A-Tdtomato-iRFP670-GFP

    Plasmid

    #111815

    Purpose

    Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

    Depositor

    Li Qian

    Article

    Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

    Inserts

    Tdtomato
    iRFP670
    GFP
    

    Use

    Cloning intermediate

    Available Since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)

    Plasmid

    #129028

    Purpose

    inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-mCherry and cloning backbone for sgRNA

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-hudTET1CD, SgRNA cloning site
    

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A), catalytic domain huTET1 inact…

    Promoter

    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-Cas9-T2A-STOP-TdT

    Plasmid

    #126425

    Purpose

    Expresses Cas9 but not TdT in mammalian cells; control for pcDNA-Cas9-T2A-TdT. (pTBL552)

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    Cas9-T2A-STOP-TdT (DNTT Streptococcus pyogenes, Human)
    

    Expression

    Mammalian

    Mutation

    alanine-6 of TdT has been changed to a STOP codon

    Promoter

    pCMV

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCherry-PHR-iSH-T2A-CIBNcaax

    Plasmid

    #173860

    Purpose

    Mammalian expression of mCherry-PHR-iSH-T2A-CIBNcaax (opto-PI3K)

    Depositor

    Takao Nakata

    Article

    Kakumoto et al PLoS One. 2013 Aug 7;8(8):e70861. doi: 1

    Insert

    mCherry-PHR-iSH-T2A-CIBNcaax
    

    Tags

    C-terminal CAAX-box and mCherry

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIVTR(T7A)-Cas9n-RT-T2A-PuroR

    Plasmid

    #172296

    Purpose

    In vitro transcription of Cas9n-RT-T2A-PuroR synthetic construct for PRIME editing

    Depositor

    Patrik Verstreken

    Insert

    Cas9n-RT-T2A-PuroR
    

    Use

    CRISPR

    Mutation

    H840A (Cas9)

    Promoter

    T7 promoter A-initiating

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBS-KS-attB2-SA(0)-T2A-QF2-Hsp70

    Plasmid

    #62944

    Purpose

    Creating QF2 drivers from MiMIC lines with inserts into coding introns in Phase 0

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-QF2-Hsp70
    

    Available Since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBS-KS-attB2-SA(2)-T2A-QF2-Hsp70

    Plasmid

    #62946

    Purpose

    Creating QF2 drivers from MiMIC lines with inserts into coding introns in Phase 2

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-QF2-Hsp70
    

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE2-TetOminiCMV-Sox2-17-t2a-Klf4

    Plasmid

    #193348

    Purpose

    tetO-S*K (Dox-inducible reprogramming lentiviral vector expressing mouse super-Sox + Klf4)

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    Sox2-17-t2a-Klf4 (Klf4, Sox2 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Sox2-17 is engineered highly cooperative chimeric…

    Promoter

    TetO mini CMV

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV hSyn1 CaRhAC T2A tDimer

    Plasmid

    #101722

    Purpose

    - humanized - variant of pAAV hSyn YFP-CaRhAC Addgene # 101721– less reliable in hippocampal neurons

    Depositor

    Thomas Oertner

    Article

    Scheib et al Nat Commun. 2018 May 24;9(1):2046. doi:

    Inserts

    CatRhAC
    red fluorescent protein
    

    Use

    AAV

    Mutation

    E497K,C569D

    Promoter

    human Synapsin1 promotor and ribosomal skip seque…

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti-EFS-hTSC1-T2A-Bsd

    Plasmid

    #193199

    Purpose

    Lentiviral vector expressing human TSC1 cDNA (driven by EFS promoter) and a blasticidin resistance marker

    Depositor

    Julien Sage

    Article

    Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

    Insert

    TSC1 (TSC1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CAG-miniSOG-T2A-dTomato

    Plasmid

    #193796

    Purpose

    Expresses miniSOG in the cytosol of mammalian cells, joined by a T2A cleavage site with dTomato

    Depositor

    Xiao Wang

    Article

    Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

    Insert

    miniSOG
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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