We narrowed to 2,400 results for: T2A

1. pBS-KS-attB2-SA(1)-T2A-QF2-Hsp70

   Plasmid

   #62945

   Purpose

   Creating QF2 drivers from MiMIC lines with inserts into coding introns in Phase 1

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-QF2-Hsp70
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV hSyn1 CaRhAC T2A tDimer

   Plasmid

   #101722

   Purpose

   - humanized - variant of pAAV hSyn YFP-CaRhAC Addgene # 101721– less reliable in hippocampal neurons

   Depositor

   Thomas Oertner

   Article

   Scheib et al Nat Commun. 2018 May 24;9(1):2046. doi:

   Inserts

   CatRhAC
   red fluorescent protein
   

   Use

   AAV

   Tags

   Expression

   Mutation

   E497K,C569D

   Promoter

   human Synapsin1 promotor and ribosomal skip seque…

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIVTR(T7A)-Cas9n-RT-T2A-PuroR

   Plasmid

   #172296

   Purpose

   In vitro transcription of Cas9n-RT-T2A-PuroR synthetic construct for PRIME editing

   Depositor

   Patrik Verstreken

   Insert

   Cas9n-RT-T2A-PuroR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   H840A (Cas9)

   Promoter

   T7 promoter A-initiating

   Available since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. FSW-ALDH2-E504K-T2A-EGFP

   Plasmid

   #197037

   Purpose

   Express ALDH2 with E504K mutation from hSyn1 promoter, co-expressed with EGFP

   Depositor

   Ronald Hart

   Article

   Sun et al Aging Cell. 2023 Feb;22(2):e13772. doi:

   Insert

   ALDH2-E504K
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   E504K

   Promoter

   Available since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE2-TetOminiCMV-Sox2-17-t2a-Klf4

   Plasmid

   #193348

   Purpose

   tetO-S*K (Dox-inducible reprogramming lentiviral vector expressing mouse super-Sox + Klf4)

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   Sox2-17-t2a-Klf4 (Klf4, Sox2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Sox2-17 is engineered highly cooperative chimeric…

   Promoter

   TetO mini CMV

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-G12-CheRiff-T2A-mCherry

   Plasmid

   #170275

   Purpose

   CheRiff-T2A-mCherry construct expression in mammlian cells. pAAV vector. Reporter plasmid.

   Depositor

   Vladislav Verkhusha

   Article

   Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

   Insert

   CheRiff-T2A-mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   11xUAS with TATA-box minimal promoter

   Available since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti-EFS-hTSC1-T2A-Bsd

   Plasmid

   #193199

   Purpose

   Lentiviral vector expressing human TSC1 cDNA (driven by EFS promoter) and a blasticidin resistance marker

   Depositor

   Julien Sage

   Article

   Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

   Insert

   TSC1 (TSC1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBS-KS-attB2-SA(0)-T2A-QF2-Hsp70

   Plasmid

   #62944

   Purpose

   Creating QF2 drivers from MiMIC lines with inserts into coding introns in Phase 0

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-QF2-Hsp70
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBS-KS-attB2-SA(2)-T2A-QF2-Hsp70

   Plasmid

   #62946

   Purpose

   Creating QF2 drivers from MiMIC lines with inserts into coding introns in Phase 2

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-QF2-Hsp70
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CAG-miniSOG-T2A-dTomato

    Plasmid

    #193796

    Purpose

    Expresses miniSOG in the cytosol of mammalian cells, joined by a T2A cleavage site with dTomato

    Depositor

    Xiao Wang

    Article

    Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

    Insert

    miniSOG
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDH-hSyn-IntCCCre-T2A-CopGFP

    Plasmid

    #160503

    Purpose

    Expresses IntCCCre in neurons in split intein-mediated split-Cre recombinase applications

    Depositor

    Shawn Je

    Article

    Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

    Insert

    IntCCCre
    

    Use

    Cre/Lox and Lentiviral

    Tags

    CopGFP

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    March 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. Tol2_B-act_dCas9-Vp64-T2A-Citrine

    Plasmid

    #200248

    Purpose

    Zebrafish transgenics; Plasmid encodes dCas9-Vp64 and could be stably integrated in the zebrafish genome using Tol2 transgenesis

    Depositor

    Tatjana Sauka-Spengler

    Article

    Pelea et al bioRxiv 2023.05.08.539738

    Insert

    dCas9-Vp64-T2A-Citrine
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. TRE-UBC-Crry-T2A-GFP

    Plasmid

    #205452

    Purpose

    lentiviral plasmid for expression of mouse Crry

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    Crry (Cr1l Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCXLE-dCas9VPP300-T2A-GFP-shP53

    Plasmid

    #102896

    Purpose

    EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-P300core followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    dCas9VPP300-T2A-GFP-shP53
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. Dppa1-T2A-H2B-Halo donor

    Plasmid

    #113118

    Purpose

    Donor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Dppa1 coding sequence

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    Dppa1 donor region including homology arms and T2A-H2B-Halo
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIVTR(T7A)-CRE-T2A-PuroR

    Plasmid

    #172298

    Purpose

    In vitro transcription of CRE-T2A-PuroR synthetic construct for the excision of floxed cassettes

    Depositor

    Patrik Verstreken

    Insert

    CRE-T2A-PuroR
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter A-initiating

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDH-EF1a-tetR-T2A-tSapphire

    Plasmid

    #176312

    Purpose

    construct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome t-Sapphire

    Depositor

    Irmela Jeremias

    Article

    Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

    Inserts

    tetR
    T-sapphire
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. Gr4-T2A-QF2 HDR plasmid

    Plasmid

    #140944

    Purpose

    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti Gr4 gene

    Depositor

    Leslie Vosshall

    Article

    Jove et al Neuron. 2020 Dec 23;108(6):1163-1180.e12

    Insert

    Gr4-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Gr4-right-HDR-arm
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEMT-Tyrosinase-bait-T2a-Cherry-pA

    Plasmid

    #111155

    Purpose

    knock in bait for Axolotl Tyrosinase locus

    Depositor

    Elly Tanaka

    Article

    Fei et al Stem Cell Reports. 2014 Sep 9;3(3):444-5

    Insert

    Tyrosinase-bait-T2a-Cherry-pA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. Calb1-T2A-dgCre targeting vector

    Plasmid

    #114438

    Purpose

    Target dgCre into the endogenous mouse Calb1 locus at the stop codon

    Depositor

    Hongkui Zeng

    Article

    Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

    Insert

    dgCre
    

    Use

    Mouse Targeting

    Tags

    DHFR domain and EGFP

    Expression

    Mutation

    Promoter

    none; utilizes endogenous Calb1 promoter for expr…

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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