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Plasmid#130961PurposeEngineered sgRNA-LEB4 generator including Anderson promoter J23100 for golden gate assemblyDepositorInsertsgRNA-LEB4
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAnderson promoter J23100Available sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY86
Plasmid#130949PurposeEngineered sgRNA-LEA2-WTmis2 generator circuit including promoter Plux2, 2 BoxB aptamers and 2 mis-matches in sgRNA scaffoldDepositorInsertsgRNA-LEA2-WTmis2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPlux2Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY132
Plasmid#130960PurposeEngineered sgRNA-LEB3 generator including Anderson promoter J23100 for golden gate assemblyDepositorInsertsgRNA-LEB3
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAnderson promoter J23100Available sinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY130
Plasmid#130952PurposeEngineered sgRNA-LEB1 generator including Anderson promoter J23100 for golden gate assemblyDepositorInsertsgRNA-LEB1
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAnderson promoter J23100Available sinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
R2Ct (1-520)
Plasmid#115649Purposeexpression of truncated Rag2 including the C-terminal regionDepositorInsertRag2 (1-520) (Rag2 Mouse)
UseTags8XHis-MBP-PreScissionExpressionMammalianMutationtruncated Rag2 (aa 1-520)PromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
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p075 pCS xTRPS1 delN delC119 (M)
Plasmid#11045DepositorInsertTRPS1 delN delC119 mut (trps1 Frog)
UseXenopus, mammalian, avian, and zebrafishTagsExpressionMutationaa806-1153 of xTRPS1. Two Cys residues in the GA…PromoterAvailable sinceDec. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
p076 pCS xTRPS1 delN delC320 (M)
Plasmid#11046DepositorInsertTRPS1 (trps1 Frog)
UseMammalian, avian, and zebrafishTagsExpressionMutationaa806-952 of xTRPS1. Two Cys residues in the GAT…PromoterAvailable sinceDec. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
p063 pCS xTRPS1 delta N (M)
Plasmid#11036DepositorInsertTRPS1 deltaN mut (trps1 Frog)
UseMammalian, avian, and zebrafishTagsExpressionMutationaa806-1153 of xTRPS1. Two Cys residues in the GA…PromoterAvailable sinceDec. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pET21c(+)-mtLeuRS
Plasmid#31079DepositorInsertMature form human mitochondrial leucyl-tRNA synthetase (LARS2 Human)
UseTagsHisExpressionBacterialMutationIncludes coding sequence for the mature form. Nt …PromoterAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLX303-ZIM3-KRAB-dCas9
Plasmid#154472PurposeExpresses ZIM3 KRAB domain fused to the N terminus of dCas9DepositorInsertZIM3 (ZIM3 Synthetic)
UseLentiviralTagsHAExpressionMammalianMutationIncludes ZIM3 aa 1-100PromoterAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
PHP.AX
Plasmid#195218PurposeAAV capsid for broadly targeting cell types in the CNS (neurons including both excitatory and inhibitory neurons, astrocytes)DepositorInsertSynthetic construct isolate AAV-PHP.AX VP1 gene
UseAAVTagsExpressionMammalianMutationPromoterp41Available sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRa_all-in-one
Plasmid#183695PurposeExpresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selectiveDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pR26 CAG/GFP Dest
Plasmid#74281PurposeGene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, GFP, for gateway cloningDepositorInsertRosa26 5-homology region
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pR26 CAG AsiSI/MluI
Plasmid#74286PurposeGene targeting vector for the mouse Rosa26 locus, including a CAG promoter and loxP flanked stop cassette, for cloning into AsiSI or MluiDepositorInsertRosa26 5-homology region
UseTagsExpressionMutationPromoterAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLX301-ZIM3-KRAB-PYL1
Plasmid#154761PurposeExpresses ZIM3 KRAB domain fused to PYL1DepositorInsertZIM3 (ZIM3 Human)
UseLentiviralTagsExpressionMammalianMutationIncludes ZIM3 aa 1-100PromoterCMVAvailable sinceOct. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pR26 CAG/GFP Asc
Plasmid#74285PurposeGene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette and GFP, for cloning into AscIDepositorInsertRosa26 5-homology region
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmRFP-LC3
Plasmid#21075PurposeMammalian expression of rat LC3 fused to mRFPDepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseTagsmRFPExpressionMammalianMutationFirst ATG is not included.PromoterAvailable sinceMay 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
GG-EBNA-EEA-5guides-PGK-Puro
Plasmid#102898PurposeEBNA episome plasmid for U6 promoter-driven expression of 5 gRNAs targeting EEA-motif. Includes PGK-puro selection cassette.DepositorInsertEEA-5guides-PGK-Puro
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWJB9
Plasmid#126650PurposeExpresses AdrA from a modified pLAFR3DepositorInsertadrA-8xHis/AdrA-8xHis including flanking promoter regions from pBAD30
UseTags8xHisExpressionBacterialMutationPromoterPBADAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only