We narrowed to 5,504 results for: pcas

1. AB65_pCAG.Cas9-D10A.rBGpA

   Plasmid

   #199256

   Purpose

   Expression construct encoding the SpCas9-D10A nickase

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

   Insert

   SpCas9.D10A nickase
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   CAG promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS::AtHAP2-V5

   Plasmid

   #132958

   Purpose

   Express AtHAP2 full length protein in mammalian cells

   Depositor

   Benjamin Podbilewicz

   Article

   Valansi et al J Cell Biol. 2017 Mar 6;216(3):571-581.

   Insert

   AtHAP2 (GCS1, HAP2 Mustard Weed)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-eGFP-pA

   Plasmid

   #107281

   Purpose

   donor vector backbone with constitutive eGFP

   Depositor

   Knut Woltjen

   Article

   Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

   Insert

   GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCA-G-intron-G

   Plasmid

   #40027

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Inserts

   GFP exon 1
   GFP exon 2
   beta-globin intron
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   insertion of a loxP site, nucleotides 1-274, and …

   Promoter

   CAG (chicken beta globin promoter and CMV enhance…

   Available since

   Oct. 12, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. AV15_pCAG.Cas9.gRNA.S1

   Plasmid

   #199259

   Purpose

   Expression construct encoding SpCas9 nuclease and an AAVS1-targeting guide RNA

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

   Inserts

   SpCas9 nuclease
   AAVS1-targeting gRNA
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter and RNA polymerase III promoter for …

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330-HA-dSpCas9

   Plasmid

   #92112

   Purpose

   Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive SpCas9
   

   Use

   CRISPR

   Tags

   HA tag and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   Cbh

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAFNF-tdTomato

   Plasmid

   #125575

   Purpose

   Flp-dependent expression of tdTomato in mammalian cells.

   Depositor

   Takeshi Imai

   Article

   Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

   Insert

   tdTomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCaSpeR4-tubulin-QF#7

   Plasmid

   #46127

   Depositor

   Christopher Potter

   Article

   Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

   Insert

   QF#7
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-Rad18

   Plasmid

   #119003

   Purpose

   Mammalian expression of human Rad18 and BFP

   Depositor

   Ralf Kuehn

   Article

   Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

   Insert

   Rad18
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-Gdf9

    Plasmid

    #163599

    Purpose

    Expresses GDF-9 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Gdf9 (Gdf9 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-T3-st1CAS9-pA

    Plasmid

    #131542

    Purpose

    Expresses S. thermophilus 1-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroys

    Depositor

    Wataru Fujii

    Article

    Fujii et al Biochem Biophys Res Commun. 2016 Jun 16.

    Insert

    Codon optimized st1Cas9
    

    Use

    CRISPR

    Tags

    Flag, NLS, and polyA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. KA2601_eSpCas9-G2P

    Plasmid

    #124203

    Purpose

    Expression of eSpCas9(1.1) and sgRNA

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-Chimeric_Butterfly_YR_1.0

    Plasmid

    #59801

    Purpose

    Genetically encoded fluorescent voltage indicator

    Depositor

    Thomas Knopfel

    Article

    Mishina et al Front Mol Neurosci. 2014 Sep 29;7:78. do

    Insert

    Yellow-Red Chimeric Butterfly Voltage Sensitive Fluorescent Protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-V5-NLGN2

    Plasmid

    #82538

    Purpose

    pCAG vector for expressing V5-NLGN2

    Depositor

    Alice Ting

    Article

    Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

    Insert

    NLGN2 (Nlgn2 Rat)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

15. cacna2d1 pCAGGS

    Plasmid

    #206104

    Purpose

    expression of rat alpha2delta-1 calcium channel auxillary subunit

    Depositor

    Annette Dolphin

    Insert

    cacna2d1 (Cacna2d1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/B-actin

    Available since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCaSpeR-act5cB-QF#7

    Plasmid

    #46125

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    QF#7
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. SpCas9 TadCBEb

    Plasmid

    #193832

    Purpose

    Express TadCBEb (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-CDb-SpCas9 D10A-2xUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-mWasabi-ER

    Plasmid

    #164165

    Purpose

    Expresses mWasabi fused with an ER-targeting sequence of calreticulin and the ER retrieval sequence, KDEL, under the control of CAG promoter

    Depositor

    Isei Tanida

    Article

    Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

    Insert

    mWasabi
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCas9CR4-VQR

    Plasmid

    #120224

    Purpose

    pCas9CR4 with VQR mutations for changing PAM site specificity to NGAN or NGNG from Kleinstiver et al. 2015

    Depositor

    Christopher Reisch

    Insert

    SpCas9-VQR
    

    Use

    Synthetic Biology

    Tags

    ssrA

    Expression

    Mutation

    Mutations D1135V/R1335V/T1337R

    Promoter

    pTet

    Available since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAR-IF reporter

    Plasmid

    #16628

    Depositor

    Bert Vogelstein

    Article

    Nicolaides et al Mol Cell Biol. 1998 Mar . 18(3):1635-41.

    Insert

    CAR-IF
    

    Use

    Tags

    beta-galactosidase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only