We narrowed to 5,839 results for: pcas

1. pCAGGS-GCaMP2

   Plasmid

   #18926

   Depositor

   Karel Svoboda

   Article

   Mao et al PLoS ONE. 2008 . 3(3):e1796.

   Insert

   GCaMP2
   

   Tags

   His

   Expression

   Mammalian

   Available Since

   Aug. 13, 2008

   Availability

   Academic Institutions and Nonprofits only

2. pF(UG) hSyn spCas9

   Plasmid

   #179725

   Purpose

   human synapsin (hSyn)-driven spCas9 lentiviral vector for CRISPR/HITI

   Depositor

   Edwin Chapman

   Article

   Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

   Insert

   spCas9
   

   Use

   Lentiviral

   Available Since

   Oct. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGGB-SpCas9

   Plasmid

   #210533

   Purpose

   Cloning A.thaliana codon optimized SpCas9 CDS as GreenGate module B

   Depositor

   Ortrun Mittelsten Scheid

   Article

   Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

   Insert

   SpCas9
   

   Use

   Synthetic Biology

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSAVED-CHAT_PCaspase_PCi_PC-σ

   Plasmid

   #214040

   Purpose

   Bacterial expression plasmid for SAVED-CHAT, PCaspase, PCi, and PC-σ from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   SAVED-CHAT-Pcaspase-Pci-PC-σ
   

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   lacUV5 promoter

   Available Since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. UVR8-GFPCaaX

   Plasmid

   #49805

   Purpose

   expresses UVR8 fused to GFP with a prenylation motif for membrane targeting

   Depositor

   Matthew Kennedy

   Article

   Chen et al J Cell Biol. 2013 May 13;201(4):631-40.

   Insert

   UVR8-GFPCaax (UVR8 Mustard Weed)
   

   Expression

   Mammalian

   Available Since

   Dec. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. 2X_pX458_pSpCas9(BB)-2A-GFP_SOX17_bKO

   Plasmid

   #172225

   Purpose

   Cas9-2A-GFP expression vector bearing two sgRNAs targeting the centromeric human SOX17 CTCF-boundary.

   Depositor

   Alexander Meissner

   Article

   Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

   Insert

   sgRNA
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGS-FuG-B

   Plasmid

   #67523

   Purpose

   This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. You can make type B envelope coating virus particle with HiRet by using this plasmid.

   Depositor

   Kazuto Kobayashi

   Article

   Kato et al Hum Gene Ther. 2011 Feb;22(2):197-206. d

   Insert

   Fusion Glycoprotein type B
   

   Use

   Lentiviral

   Tags

   Fusion protein of Vesicular stomatitis Indiana vi…

   Promoter

   CAGGS

   Available Since

   July 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-hPPP3R1

   Plasmid

   #179136

   Purpose

   Expression vector of human protein phosphatase 3, regulatory subunit B, alpha isoform (PPP3R1), CAG promoter, rabbit globin poly(A) signal.

   Depositor

   Masahito Ikawa

   Article

   Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

   Insert

   protein phosphatase 3, regulatory subunit B, alpha isoform (PPP3R1 Human)
   

   Expression

   Mammalian

   Available Since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-mTHC

   Plasmid

   #112620

   Purpose

   expression plasmid with CAG promoter driving multicistronic cassette H2BmTeal_p2A_HygroR_p2A_CreERt2

   Depositor

   Stefan Heller

   Article

   Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

   Inserts

   H2B-mTFP1 (H2BC3 Anthozoa, Human)
   Hygrophosphotransferase
   Cre-ERT2 fusion protein
   

   Tags

   mTeal (mTFP1)

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGGS-6017nes

    Plasmid

    #108655

    Purpose

    A PKA biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

    Depositor

    Michiyuki Matsuda

    Article

    Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

    Insert

    hyBRET-PKA-EV
    

    Tags

    XFP

    Expression

    Mammalian

    Promoter

    pCAGGS

    Available Since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-T3-hCASeGFP-pA

    Plasmid

    #59766

    Purpose

    Expresses CAS9-eGFP fusion protein in mammalian cells.

    Depositor

    Wataru Fujii

    Article

    Fujii et al Nucleic Acids Res. 2013 Aug 30.

    Insert

    CAS9
    

    Tags

    Flag and eGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGGS-6015nes

    Plasmid

    #108657

    Purpose

    A S6 Kinase biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

    Depositor

    Michiyuki Matsuda

    Article

    Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

    Insert

    hyBRET-S6K-EV
    

    Tags

    XFP

    Expression

    Mammalian

    Promoter

    pCAGGS

    Available Since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCas9-J23109

    Plasmid

    #113150

    Purpose

    Plasmid constitutively expressing Cas9 protein

    Depositor

    Chong Zhang

    Article

    Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

    Insert

    Cas9
    

    Expression

    Bacterial

    Available Since

    Dec. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCabp5-DsRed

    Plasmid

    #11157

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

    Insert

    Cabp5 promoter (Cabp5 Mouse)
    

    Tags

    DsRed2

    Expression

    Mammalian

    Available Since

    April 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCALNL-mutKir2.1

    Plasmid

    #125579

    Purpose

    Cre-dependent expression of a non-conducting mutant of Kir2.1 (FLAG-tagged) in mammalian cells.

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    Kir2.1 (Kcnj2 Mouse)
    

    Use

    Cre/Lox

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    A non-conducting mutant (GYG -> AAA).

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCALNL-Kir2.1

    Plasmid

    #125578

    Purpose

    Cre-dependent expression of Kir2.1 (FLAG-tagged) in mammalian cells.

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    Kir2.1 (Kcnj2 Mouse)
    

    Use

    Cre/Lox

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-Ssh1

    Plasmid

    #163602

    Purpose

    Expresses Protein phosphatase Slingshot homolog 1 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    ssh1 (Ssh1 Mouse)
    

    Expression

    Mammalian

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSPCas9(BB)-2A-GFP_ABCA3GFP_targeting

    Plasmid

    #188541

    Purpose

    Plasmid encoding pCas9 and gRNA targeting the endogenous human ABCA3 locus stop codon

    Depositor

    Darrell Kotton

    Article

    Sun et al Am J Respir Cell Mol Biol. 2021 Oct;65(4

    Insert

    gRNA
    

    Use

    CRISPR

    Tags

    GFP

    Promoter

    U6

    Available Since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCA-G-intron-G

    Plasmid

    #40027

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    GFP exon 1
    GFP exon 2
    beta-globin intron
    

    Expression

    Mammalian

    Mutation

    insertion of a loxP site, nucleotides 1-274, and …

    Promoter

    CAG (chicken beta globin promoter and CMV enhance…

    Available Since

    Oct. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAP03-act1p

    Plasmid

    #120233

    Purpose

    Carries promoter cassette containing act1p promoter and apramycin resistance gene that can be amplified with homology sequence for refactoring.

    Depositor

    Bradley Moore

    Article

    Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

    Insert

    promoter cassette containing act1p promoter and apramycin resistance gene
    

    Use

    Synthetic Biology

    Mutation

    aac(3)IV (apramycin resistance gene)

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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