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pcDNA3 Plk4(Sak) wt (Nigg HR9)

Plasmid

#41165

Depositor

Erich Nigg

Article

Habedanck et al Nat Cell Biol. 2005 Nov;7(11):1140-6.

Insert

Plk4 (PLK4 Human)

Tags

3xMyc and PreScission Site

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

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pSVL(D2m)

Plasmid

#29336

Depositor

John Taylor

Article

Kuo et al J Virol. 1989 May . 63(5):1945-50.

Insert

Dimer of HDV genome with 2 nucleotide deletion in delta antigen

Expression

Mammalian

Mutation

Contains a dimer of the complete HDV genome excep…

Available Since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

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pSEVA221TadA*-T7RNAP

Plasmid

#167980

Purpose

Expresses fusion protein TadA*-T7RNAP in E. coli

Depositor

Luis Ángel Fernández

Article

Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

Insert

TadA*-T7RNAP

Tags

T7 tag and T7RNAP

Expression

Bacterial

Promoter

TetR-Ptet

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pWS3799-gRNA-Csy4-template

Plasmid

#182827

Purpose

SpCas9 gRNA-Csy4 template

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Insert

SpCas9 gRNA scaffold + Csy4 sequence

Use

CRISPR

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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PcDNA3.1-2Strep

Plasmid

#210343

Purpose

Empty Vector for molecular cloning

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

2Strep tag

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-SUMO-DogTag

Plasmid

#105629

Purpose

Plasmid for recombinant expression in bacteria of SUMO domain fused to DogTag, permitting covalent linkage to SnoopTagJr

Depositor

Mark Howarth

Article

Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3

Insert

SUMO-DogTag

Expression

Bacterial

Promoter

T7

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pCMU-TGNVHAr

Plasmid

#61178

Purpose

Fluorescent reporter for trans-Golgi network AtVHA-a1-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtVHA-a1

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pETZZtevPP7CP(C68A,C73A)

Plasmid

#27548

Depositor

Kathleen Collins

Article

Hogg et al RNA. 2007 Jun . 13(6):868-80.

Insert

Pseudomonas phage PP7 coat protein (cp Pseudomonas phage PP7)

Tags

TEV protease cleavage site, six histidine tag, an…

Expression

Bacterial

Mutation

changed two cysteine to alanine (C68A,C73A) to in…

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pCN38-ktrA-yfp

Plasmid

#227636

Purpose

c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.

Depositor

Angelika Grundling

Article

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

ktrA-yfp

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pJH2930

Plasmid

#175283

Purpose

Pges-1 DAF-16a unc-54 3' UTR C.elegans gut expression of isoform a of DAF-16

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

DAF-16a (daf-16 Nematode)

Expression

Worm

Promoter

Pges-1

Available Since

Dec. 3, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pWS5397-CRISPRai-NatR

Plasmid

#182836

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - NatR marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCMU-APr

Plasmid

#61182

Purpose

Fluorescent reporter for apoplast MtBCP1sp-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtBCPsp-mCherry

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-3HA-TRP1

Plasmid

#41599

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

TRP1 (TRP1 Budding Yeast)

Use

Yeast genomic targeting

Tags

3xHA and yeast ADH1 terminator

Available Since

May 15, 2013

Availability

Academic Institutions and Nonprofits only

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p201B Cas9

Plasmid

#59177

Purpose

Cas9 driven by double 35S, BAR for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

Depositor

Wayne Parrott

Article

Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

Inserts

Cas9
BAR

Use

CRISPR

Promoter

2x35S

Available Since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pFastBac-His-Tipin

Plasmid

#22891

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

Insert

Tipin (TIPIN Human)

Tags

His

Expression

Insect

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
GFP-Myo 9b

Plasmid

#134907

Purpose

Expression of rat Myo 9b (Myr 5) in mammalian cells.

Depositor

Martin Bähler

Article

Reinhard et al EMBO J. 1995 Feb 15;14(4):697-704.

Insert

Myo 9b (rat) with 3' UTR (Myo9b Rat)

Tags

EGFP

Expression

Mammalian

Promoter

CMV IE

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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hBax S184A C3-EGFP

Plasmid

#19742

Depositor

Richard Youle

Article

Nechushtan et al EMBO J. 1999 May 4. 18(9):2330-41.

Insert

Bax S184A (BAX Human)

Tags

GFP

Expression

Mammalian

Mutation

Serine 184 to Alanine

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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543-p3E-2A-mcherrypA

Plasmid

#26031

Depositor

Nathan Lawson

Article

Villefranc et al Development. 2013 Apr;140(7):1497-506. d

Insert

mCherry

Use

Entry vector

Available Since

Aug. 9, 2010

Availability

Academic Institutions and Nonprofits only

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pBSV2_PresT-mCherryBb

Plasmid

#118238

Purpose

Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes resT promoter driven mCherry

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

resT promoter driven mCherry

Expression

Bacterial

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pScPPX1

Plasmid

#233713

Purpose

Bacterial expression of the yeast PPX1 gene

Depositor

Michael Downey

Article

Baijal et al bioRxiv 2025.02.12.637445

Insert

ScPpx1 (PPX1 Budding Yeast)

Expression

Bacterial

Available Since

May 7, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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