We narrowed to 166,390 results for: addgene

1. pAAV-syn-IRES-hrGFP

   Plasmid

   #107549

   Purpose

   high transduction efficiency AAV-mediated synapsin promoter-dependent expression IRES-hrGFP

   Depositor

   Hélène Marie

   Article

   Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

   Type

   Empty backbone

   Use

   AAV

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

2. pX459-sgRNA2-CTCF-prom

   Plasmid

   #195104

   Purpose

   Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting upstream of the human CTCF promoter. Puromycin selection (2A-fusion).

   Depositor

   Job Dekker

   Article

   Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

   Insert

   sgRNA against CTCF promoter
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX459-sgRNA1-CTCF-prom

   Plasmid

   #195103

   Purpose

   Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting upstream of the human CTCF promoter. Puromycin selection (2A-fusion).

   Depositor

   Job Dekker

   Article

   Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

   Insert

   sgRNA against CTCF promoter
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. ATP5O_pCSdest

   Plasmid

   #53812

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   ATP5O (ATP5PO Human)
   

   Expression

   Mammalian

   Promoter

   SP6 and CMV

   Available Since

   March 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

5. CMVp-ECFP-Triplex-28-8xmiRNA-BS-28-pA (Construct 22)

   Plasmid

   #55199

   Purpose

   CFP reporter along with FF4 miRNA binding sites and 28 nt sequences

   Depositor

   Timothy Lu

   Article

   Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

   Insert

   ECFP
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV V5-Vkorc1l1

   Plasmid

   #175148

   Purpose

   Lentiviral expression of V5-tagged mouse Vkorc1l1

   Depositor

   Larry Gerace

   Article

   Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

   Insert

   Vkorc1l1 (Vkorc1l1 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNH12 (pmyo-2::MacQ::mCitrine)

   Plasmid

   #130274

   Purpose

   Expresses the eFRET-based voltage sensor MacQ::mCitrine in the pharynx of C. elegans.

   Depositor

   Alexander Gottschalk

   Article

   Azimi Hashemi et al Proc Natl Acad Sci U S A. 2019 Aug 1. pi

   Insert

   MacQ::mCitrine
   

   Tags

   mCitrine

   Expression

   Worm

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV Tmem161a-V5

   Plasmid

   #175149

   Purpose

   Lentiviral expression of mouse Tmem161a-V5

   Depositor

   Larry Gerace

   Article

   Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

   Insert

   Tmem161a (Tmem161a Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Jan. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT2-cryR;dll4CE1-P1Egfp

   Plasmid

   #90146

   Purpose

   contains dll4 enhancer 1 sequence, and dll4 specific gene promoter driving expression of eGFP

   Depositor

   Nathan Lawson

   Article

   Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

   Insert

   dll4E1P1Egfp (dll4 Zebrafish)
   

   Tags

   EGFP

   Expression

   Bacterial

   Available Since

   May 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-CAG-dDIO-lck-smMyc-4x6T

    Plasmid

    #196421

    Purpose

    Dre-dependent AAV expression of membrane-targeted Myc spaghetti monster reporter preferentially in astrocytes; astrocyte selectivity generated with 4x6T miRNA targeting cassette

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Insert

    Lck-smMyc
    

    Use

    AAV; Dre/rox; astrocyte-selective

    Tags

    Lck

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. LLP779_αGCN4-VP64-CO

    Plasmid

    #211772

    Purpose

    SunTag counterpart binding domain, αGCN4, fused to transcriptional activator VP64, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    aGCN4-VP64
    

    Tags

    3xTy1

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect VP64 e…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSCV-Atoh1wt-IRES-RFP

    Plasmid

    #33334

    Depositor

    Martine Roussel, Charles Sherr

    Article

    Ayrault et al Cancer Res. 2010 Jul 1;70(13):5618-27. E

    Insert

    Atoh1 (Atoh1 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

13. DsRed2_pLENTI-CAG-IRES-GFP

    Plasmid

    #177010

    Purpose

    Mammalian lentiviral expression vector encoding DsRed2

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    DsRed2
    

    Use

    Lentiviral

    Promoter

    CAG

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. 8xCTS1_WT-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_WT-hutRNApro_SV40polyA

    Plasmid

    #124546

    Purpose

    Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    WT-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_WT-hutRNApro
    

    Expression

    Mammalian

    Mutation

    WT, WT

    Promoter

    Minimal ADE

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT2-cryR;lmo2CE1-P1Egfp

    Plasmid

    #90153

    Purpose

    contains lmo2 enhancer sequence, and lmo2 specific gene promoter driving expression of eGFP

    Depositor

    Nathan Lawson

    Article

    Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

    Insert

    lmo2E1P1Egfp (lmo2 Zebrafish)
    

    Tags

    EGFP

    Expression

    Bacterial

    Available Since

    June 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. MSP2372

    Plasmid

    #73196

    Purpose

    Human expression plasmid for SpCas9(R661A/Q695A/Q926A) variant: CMV-T7-humanSpCas9(R661A, Q695A, Q926A)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 (R661A/Q695A/Q926A)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    R661A, Q695A, and Q926A in SpCas9

    Promoter

    CMV

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. lentiCRISPR-v2-FE-PuroR

    Plasmid

    #186746

    Purpose

    Modified lentiCRISPRv2 3rd generation lentiviral backbone (Addgene Cat# 52961) containing an FE hairpin

    Depositor

    Richard Sherwood

    Article

    Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. BACE2_pCSdest

    Plasmid

    #53915

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    BACE2 (BACE2 Human)
    

    Expression

    Mammalian

    Promoter

    SP6 and CMV

    Available Since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pAG247

    Plasmid

    #33200

    Depositor

    David Bartel

    Article

    Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

    Insert

    LRIG1 (LRIG1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Promoter

    SV40 early enhancer/promoter

    Available Since

    Jan. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pT2-cryR;lmo2P1Egfp

    Plasmid

    #90162

    Purpose

    contains lmo2 specific gene promoter driving expression of eGFP

    Depositor

    Nathan Lawson

    Article

    Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

    Insert

    lmo2P1Egfp (lmo2 Zebrafish)
    

    Tags

    EGFP

    Expression

    Bacterial

    Available Since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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