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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,276 results for: nes

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Showing: 7201 - 7220 of 16276 results

  1. pSHUT4-eYFP

    Plasmid
    #133094
    Purpose
    Agrobacterium T-DNA overexpression cassette comprising constitutive TEF-1alpha fused to reporter eYFP. Integrates near beta-tubulin gene in F. solani genome. Replace eYFP w/ XhoI BamHI
    Depositor
    Jens Laurids Sørensen
    Publication
    Nielsen et al Fungal Biol Biotechnol. 2019 Dec 11;6:25
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    enhanced yellow flourescent protein
    Use
    Fungal expression
    Promoter
    TEF-1α
    Available since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    133094-plasmid-map-sequence-id-266874

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  2. EF1a_MYC Ser62Ala_P2A_Hygro_Barcode

    Plasmid
    #120514
    Purpose
    Barcoded lentiviral vector to express MYC Ser62Ala in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
    Depositor
    Prashant Mali
    Publication
    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(
    Insert
    MYC Ser62Ala (MYC Human)
    Use
    Lentiviral
    Mutation
    Point mutation changing Serine to Alanine at amin…
    Promoter
    EF1a
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    120514-plasmid-map-sequence-id-234976

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  8. pRSET a sfMatryoshCaMP6s-T78H

    Plasmid
    #100021
    Purpose
    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference LSSmOrange nested within the reporting superfolder cpGFP-T78H
    Depositor
    Wolf Frommer
    Publication
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    sfMatryoshCaMP6s
    Tags
    6x HIS tag and Xpress_EK tag
    Expression
    Bacterial
    Mutation
    cpEGFP replaced with superfolder cpGFP and T78H m…
    Promoter
    T7
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100021-plasmid-map-sequence-id-194294

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  16. pCNL-N1_PTS2

    Plasmid
    #65676
    Purpose
    Expresses PTS2-CNL in mammalian cells
    Depositor
    Yasushi Okada
    Publication
    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    peroxisomal targeting signal 2
    Use
    Luciferase
    Tags
    CNL
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    65676-plasmid-map-sequence-id-116027

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  18. pAC148-pCR8-dCas9VP96

    Plasmid
    #48220
    Purpose
    dCas9VP96 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression
    Depositor
    Rudolf Jaenisch
    Publication
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Insert
    dCas9(D10A;H840A) fusion with VP96 activation domain
    Use
    CRISPR and Gateway Cloning
    Tags
    HA Tag and VP96
    Mutation
    D10A;H840A
    Available since
    Sept. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    48220-plasmid-map-sequence-id-69505

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  19. NEDD5

    Plasmid
    #25184
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NEDD5:E13-Q320-B3_W260A (SEPTIN2 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids E13-Q320. W260A mutation.
    Available since
    Aug. 24, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. HERC2 (3KCI)

    Plasmid
    #25344
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    herc2.3951.4321 (HERC2 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    contains amino acid residues 3951-4321
    Available since
    Aug. 17, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 7201 - 7220 of 16276 results