We narrowed to 13,447 results for: sequence
-
Plasmid#83426Purposepromoter reporter construct to test 5' flanking sequencesDepositorInserthuman Sgk1 gene 5' flanking seq + 117 nt of the 5' UTR (SGK1 Human)
UseLuciferaseTagsLuciferasePromotersgkAvailable SinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-mCherry-CAAX
Plasmid#166229PurposeLentiviral plasmid for expression of mCherry with a C-terminal CAAX polybasic sequence from KRras targeting mCherry to the plasma membrane.DepositorInsertmCherry-CAAX
UseLentiviralTagsCAAXExpressionMammalianPromoterCMVAvailable SinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
-
ULK1-Halo HRD
Plasmid#207548PurposeHomologous recombination donor to integrate a 3xFLAG-HaloTag at the C-terminus of the endogenous ULK1 locus in human cellsDepositorInsertHaloTag followed by a PolyA signal and PuroR cassette flanked by human ULK1 locus sequences
TagsHaloTagExpressionMammalianPromoterEndogenousAvailable SinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pV1F(Spec)
Plasmid#205086PurposeProkaryotic expressing of AtVIP1 fused with a sequence encoding a T4SS translocation tag in A. tumefaciens.DepositorInsertAtVIP1
ExpressionBacterialAvailable SinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
p5E-4xnrUAS
Plasmid#87120PurposeMultisite gateway entry clone containing 4 non-repetitive UAS (upstream activation sequence) elementsDepositorInsert4xnrUAS (non repetitive)
UseGateway vectorAvailable SinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT3TS_Transposase
Plasmid#109768PurposeCodon optimised transposase sequence in an optimal vector for in vitro transcription. Contains the T3 promoter and synthetic polyA tract.DepositorInsertTol2 Transposase
UseZebrafish plasmidsAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSG5-ERRbeta2
Plasmid#52186PurposeExpresses human ERRbeta2 splice variant in mammalian cellsDepositorInsertEstrogen-related receptor beta (ESRRB Human)
ExpressionMammalianMutationwild type sequence; codon optimized to exclude Ec…PromoterSV40Available SinceApril 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLEx-TetOn3G-3xHA-FLEx
Plasmid#133729PurposePlasmid contains the floxed TetOn3G-transactivator sequence for expression of TetOn3G in the presence of Cre recombinase.DepositorInsertTetOn3G-3xHA (floxed & inverted)
UseAAVTags3xHA-tagExpressionMammalianPromoterpCMVAvailable SinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
UASGAL-Pgal
Plasmid#60338PurposeContains a promoter of activating sequence derived from native galactose promoter coupled with full length native galactose promoterDepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…ExpressionYeastAvailable SinceNov. 6, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAV-H1promoter-2xTetO-High_ribosome_load-Tornado-2xALFA-10xSunTag
Plasmid#231594PurposeExpress high ribosome loading circular RNA translation reporter with 2xALFATag-10xSunTag under H1 promoterDepositorInsertKozak sequence-Tornado-10xSunTag-2xALFATag
ExpressionMammalianAvailable SinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-2xMreBN-mCherry-His
Plasmid#133626PurposeExpression vector for mCherry fused to a tandem repeat of the E. coli MreB membrane targeting sequence (MTS) (N-terminal fusion) and a C-terminal His-tag for purification.DepositorInsert2xMreB-mCherry-His
TagsHisExpressionBacterialAvailable SinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC22A1
Plasmid#132034PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC22A1 (SLC22A1 Human)
ExpressionMammalianAvailable SinceOct. 25, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
ii-Str_SBP-mCherry-GPI
Plasmid#65297Purposesynchronize trafficking of mCherry-GPI from the ER (RUSH system)DepositorInsertGPI anchor
TagsIL-2 Signal Sequence and Streptavidin Binding Pro…ExpressionMammalianPromoterCMVAvailable SinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
T7-LacO-QF-T7-TetO-TetR_p15A
Plasmid#171661PurposeExpresses QF and/or TetR in BL21(DE3) E. coli according to the presence of aTc. There is no T7 terminator sequence between the QF- and TetR-containing circuits. Contains a p15A origin of replication.DepositorInsertQF-T7-TetO-TetR
ExpressionBacterialPromoterT7Available SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
actc1b-Lifeact-mKate2
Plasmid#109493PurposeMuscle specific zebrafish expression of mKate2 fused to the actin binding lifeact sequenceDepositorInsertactc1b-Lifeact-mKate2
UseZebrafish plasmidsAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet-H_NES_C1
Plasmid#85061PurposeIn vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)DepositorInsertNES
ExpressionMammalianPromoterCMVAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLicC-MBP-APETx2
Plasmid#72668PurposePeriplasmic expression of APETx2 in E.coliDepositorInsertAPETx2
TagsHis tag, MBP, MalE signal sequence for periplasmi…ExpressionBacterialPromoterT7Available SinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
Fragment 1 Null
Plasmid#198729PurposeGateway-compatible gRNA backbone . Template for the addition of target sequences to produce 2x gRNA.DepositorInsertgRNA expression cassette in pENTR4
UseGateway-compatible entry vectorAvailable SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDS257
Plasmid#34971PurposeExpresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep.DepositorInsertMid2(SS/TM)-GFP-LOVpep
ExpressionYeastAvailable SinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only