We narrowed to 7,723 results for: ALP

1. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry R1392E

   Plasmid

   #163993

   Purpose

   Mammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 CARD (NLRP1 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   R1392E

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry R1386E

   Plasmid

   #163992

   Purpose

   Mammalian expression vector encoding codon-optimized human NLRP1 CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 CARD (NLRP1 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   R1386E

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-Tac-STC(5A)

   Plasmid

   #162491

   Purpose

   Expresses non-O-glycosylated partial length Tac (stem region, TMD and cytosolic tail) with GFP tag in mammalian cells

   Depositor

   Lei Lu

   Article

   Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

   Insert

   partial length Tac (stem region with mutations, TMD and cytosolic tail) (IL2RA Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Tac is in partial length with its stem region, TM…

   Available Since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-GFP-EBA175-Flag(E1418K/A1419R/S1421R/P1424Q/Y1426S)

   Plasmid

   #155010

   Purpose

   Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 E1418K/A1419R/S1421R/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1

   Depositor

   Sinisa Urban

   Article

   Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

   Insert

   PfEBA175
   

   Tags

   Flag, GFP, and signal peptide (residues 1 to 32) …

   Expression

   Mammalian

   Mutation

   E1418K/A1419R/S1421/P1424Q/Y1426S; insert codes f…

   Promoter

   CMV

   Available Since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. INCTbiosyn-pEF-GFP(rc)5

   Plasmid

   #127506

   Purpose

   Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 5 attachment sites.

   Depositor

   Martin Bonamino

   Article

   Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

   Insert

   EGFP reverse complement coding sequence flanked by attB/attP Integrase 5 attachment sites.
   

   Expression

   Mammalian

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. INCTbiosyn-pEF-GFP(rc)7

   Plasmid

   #127507

   Purpose

   Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 7 attachment sites.

   Depositor

   Martin Bonamino

   Article

   Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

   Insert

   EGFP reverse complement coding sequence flanked by attB/attP Integrase 7 attachment sites.
   

   Expression

   Mammalian

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. INCTbiosyn-pEF-GFP(rc)4

   Plasmid

   #127505

   Purpose

   Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 4 attachment sites.

   Depositor

   Martin Bonamino

   Article

   Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

   Insert

   EGFP reverse complement coding sequence flanked by attB/attP Integrase 4 attachment sites.
   

   Expression

   Mammalian

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLL3.7-EF-EYFP-YAP1_R89A-PolyA

   Plasmid

   #112291

   Purpose

   Fluorescently labels human YAP1-2gamma isoform (504 aa) R89A mutant

   Depositor

   Erik Sahai

   Article

   Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

   Insert

   EYFP-YAP1 (YAP1 Human)
   

   Use

   Lentiviral

   Mutation

   Human YAP1-2gamma with Arginine 89 to Alanine

   Promoter

   EF-1 alpha

   Available Since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLL3.7-EF-EYFP-YAP1_L91A-PolyA

   Plasmid

   #112292

   Purpose

   Fluorescently labels human YAP1-2gamma isoform (504 aa) L91A mutant

   Depositor

   Erik Sahai

   Article

   Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

   Insert

   EYFP-YAP1 (YAP1 Human)
   

   Use

   Lentiviral

   Mutation

   Human YAP1-2gamma with Leucine 91 to Alanine

   Promoter

   EF-1 alpha

   Available Since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLL3.7-EF-EYFP-YAP1_F95A-PolyA

    Plasmid

    #112293

    Purpose

    Fluorescently labels human YAP1-2gamma isoform (504 aa) F95A mutant

    Depositor

    Erik Sahai

    Article

    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

    Insert

    EYFP-YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Mutation

    Human YAP1-2gamma with Phenylalanine 95 to Alanine

    Promoter

    EF-1 alpha

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5-FRT-TO-vsv-Incenp d543-746-GFP

    Plasmid

    #108503

    Purpose

    inducible expression of vsv-INCENP d543-746-EGFP

    Depositor

    Susanne Lens

    Article

    van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

    Insert

    INCENP d543-746 (INCENP Human)
    

    Tags

    EGFP and VSV

    Expression

    Mammalian

    Mutation

    delta alpha helix

    Promoter

    CMV

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. CSK22_HUMAN_D0

    Plasmid

    #79704

    Purpose

    This plasmid encodes the kinase domain of CSK22. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    CSK22 (CSNK2A2 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

13. DGKA gRNA (BRDN0001144932)

    Plasmid

    #76608

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human DGKA

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    DGKA (DGKA Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. DGKA gRNA (BRDN0001147297)

    Plasmid

    #76605

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human DGKA

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    DGKA (DGKA Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. DGKA gRNA (BRDN0001148437)

    Plasmid

    #76606

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human DGKA

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    DGKA (DGKA Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. DGKA gRNA (BRDN0001145658)

    Plasmid

    #76607

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human DGKA

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    DGKA (DGKA Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRRLHygro-pEF1a-p53ashL344A-mKate2-splitmVenusC

    Plasmid

    #69585

    Purpose

    Expresses mutated p53-L344A tagged with mKate2 and split C-term mVenus

    Depositor

    Galit Lahav

    Article

    Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

    Insert

    p53-L344A (TP53 Human)
    

    Use

    Lentiviral

    Tags

    mKate2 and split mVenus - C term

    Expression

    Mammalian

    Mutation

    p53 L344A mutation that prevents tetramerization …

    Promoter

    EF1alpha

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEF6-Acly

    Plasmid

    #70765

    Purpose

    Expresses mouse ATP-citrate lyase in mammalian cells

    Depositor

    Kathryn Wellen

    Article

    Lee et al Cell Metab. 2014 Aug 5;20(2):306-19. doi

    Insert

    ATP Citrate Lyase (Acly Mouse)
    

    Tags

    myc-epitope

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEF6-Acly(S455D)

    Plasmid

    #70768

    Purpose

    Expresses phosphomimetic mouse ATP-citrate lyase in mammalian cells

    Depositor

    Kathryn Wellen

    Article

    Lee et al Cell Metab. 2014 Aug 5;20(2):306-19. doi

    Insert

    ATP Citrate Lyase (Acly Mouse)
    

    Tags

    myc-epitope

    Expression

    Mammalian

    Mutation

    S455D

    Promoter

    EF1alpha

    Available Since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEF6-Acly(S455A)

    Plasmid

    #70767

    Purpose

    Expresses phosphomutant mouse ATP-citrate lyase in mammalian cells

    Depositor

    Kathryn Wellen

    Article

    Lee et al Cell Metab. 2014 Aug 5;20(2):306-19. doi

    Insert

    ATP Citrate Lyase (Acly Mouse)
    

    Tags

    myc-epitope

    Expression

    Mammalian

    Mutation

    S455A

    Promoter

    EF1alpha

    Available Since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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