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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,276 results for: nes

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Showing: 7261 - 7280 of 16276 results

  4. pPBO.ACT006

    Plasmid
    #182716
    Purpose
    Constituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region, with eGFP for cloning
    Depositor
    Joseph Schoeniger
    Publication
    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666
    Insert
    CasRx crRNA cloning backbone
    Use
    CRISPR
    Tags
    GFP in cloning site of crRNA for easier screening…
    Expression
    Bacterial
    Mutation
    Catalytically deactivated R295A, H300A, R849A, H8…
    Promoter
    pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)
    Available since
    Aug. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    182716-plasmid-map-sequence-id-361297

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  9. pTO_Snf7_LAP

    Plasmid
    #101851
    Purpose
    Tet-inducible expression of Snf7 from S. cerevisiae with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration
    Depositor
    Daniel Gerlich
    Publication
    Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d
    Insert
    SNF7 (SNF7 Budding Yeast)
    Use
    Gateway cloning, flp-frt recombination, tet-induc…
    Tags
    Localization and Affinity Purification (LAP) tag
    Expression
    Bacterial and Mammalian
    Promoter
    CMV
    Available since
    Dec. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    101851-plasmid-map-sequence-id-200251

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  13. ZIKV isolate VE_Ganxian2016 NS2B-NS3 protease co-expression of NS2B and NS3 protease domain

    Plasmid
    #228663
    Purpose
    Bacterial co-expression of the NS2B and NS3 proteases from Zika virus for crystallization assays in P43 2 2 (PDB:8PN6)
    Depositor
    ASAP Discovery, Frank von Delft
    Insert
    Zika virus portion of NS2B protease coexpressed with NS3 protease
    Tags
    His-GST-TEV
    Expression
    Bacterial
    Available since
    Feb. 18, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article
    228663-plasmid-map-sequence-id-461604

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  14. pLV-SFFV-KTN1-WPRE-UbC-Emerald

    Plasmid
    #225941
    Purpose
    Lentiviral vector plasmid expressing human kinectin 1 (KNT1) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis, Evan Reid
    Inserts
    KTN1 (KTN1 Human)
    Emerald
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225941-plasmid-map-sequence-id-446792

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  16. Lenti-UbC-FKRP-EF1a-BSD

    Plasmid
    #205150
    Purpose
    Express UbC-driven FKRP and EF-1a driven BSD
    Depositor
    Monkol Lek
    Insert
    Fukutin-related protein (FKRP Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    UbC
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    205150-plasmid-map-sequence-id-405993

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  17. pEGFP_D2A_tdTomato

    Plasmid
    #184045
    Purpose
    Expresses EGFP and tdTomato in mammalian cells under control of a CMV promoter by using a dual 2A peptide sequence (P2AT2A)
    Depositor
    Angela Pannier
    Publication
    Kozisek et al J Biol Eng. 2023 Dec 7;17(1):76. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Enhanced Green Fluorescent Protein
    tdTomato
    Dual 2A Peptide Sequence
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    184045-plasmid-map-sequence-id-363159

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  19. pT26CfpSox10G4FER3(#1048)

    Plasmid
    #184073
    Purpose
    cyclofen-inducible GAL4 activation in zebrafish permanent transgenic
    Depositor
    Sophie Vriz
    Insert
    Gal4bdFF-ERT2
    Use
    Zebrafish transgenesis (blue heart)
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    Zf-Sox10
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    184073-plasmid-map-sequence-id-365954

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  20. pCAG-mEGFP-CaMKIIa (T305D/T306D)

    Plasmid
    #127611
    Purpose
    Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T305D/T306D)
    Depositor
    Ryohei Yasuda
    Publication
    Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    changed Threonine 305/306 to Alanine
    Promoter
    pCAG
    Available since
    Jan. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    127611-plasmid-map-sequence-id-250748

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Showing: 7261 - 7280 of 16276 results