161,424 results

1. piggyBac-ABE8.20-NG

   Plasmid

   #226866

   Purpose

   Expresses dox-inducible ABE8.20-NG for mammalian cells. piggyBac vector

   Depositor

   Alexis Komor

   Article

   Osgood et al Methods in Enzymology, February 5, 2025

   Insert

   ABE8.20-NG
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Tet-On-TRE3G

   Available since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSCV-IRES-CFP II (pMIC II)

   Plasmid

   #52109

   Purpose

   a bicistronic IRES-CFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP

   Depositor

   Dario Vignali

   Article

   Holst et al Nat Protoc. 2006;1(1):406-17.

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   IRES-ECFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. TFORF3083

   Plasmid

   #144559

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   FOSL2 (FOSL2 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. QPM-sgR (pTaU3)

   Plasmid

   #140448

   Purpose

   For sgRNA expression in monocotyledons protoplasts

   Depositor

   Caixia Gao

   Article

   Lin et al Nat Biotechnol. 2020 May;38(5):582-585.

   Insert

   TaU3-sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   TaU3

   Available since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenLox_U6 p53

   Plasmid

   #59360

   Purpose

   Lentiviral expression vector: p53 shRNA under control of mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor expression.

   Depositor

   Peter Scheiffele

   Article

   Chih et al Science. 2005 Feb 25;307(5713):1324-8. E

   Inserts

   shRNA p53 (mouse) (Trp53 Mouse)
   eGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and mouse U6

   Available since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti HRE-Luc pGK Hygro

   Plasmid

   #118706

   Purpose

   reporter plasmid HIF-responsive promoter (3xHRE) wt- fused to firefly luciferase

   Depositor

   William Kaelin

   Article

   Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

   Insert

   HRE (HIF-responsive promoter (3xHRE)-luc
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-MTS-jAspSnFR3-mRuby3

   Plasmid

   #203492

   Purpose

   Mitochondrial directed jAspSnFR3-mRuby3 in lentiviral expression vector

   Depositor

   Lucas Sullivan

   Article

   Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

   Insert

   MTS-jAspSnFR3-mRuby3
   

   Use

   Lentiviral

   Tags

   His-tag, Mitochondrial localization signal, and m…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. psgRNA_EcoWG1

   Pooled Library

   #131625

   Purpose

   Improved genome-wide knockdown of E. coli genes

   Depositor

   David Bikard

   Article

   Calvo-Villamanan et al Nucleic Acids Res. 2020 Apr 30. pii: 582

   Expression

   Bacterial

   Species

   Escherichia coli

   Use

   CRISPR

   Available since

   May 12, 2020

   Availability

   Academic Institutions and Nonprofits only

9. pAAV-Ef1a-DIO ChETA-EYFP (AAV1)

   Viral Prep

   #26968-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV-Ef1a-DIO ChETA-EYFP (#26968). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO ChETA-EYFP plasmid DNA. EF1a-driven, Cre-dependent, ChETA fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Article

   Gunaydin et al Nat Neurosci. 2010 Mar;13(3):387-92. doi

   Promoter

   EF1a

   Tags

   EYFP (Cre-dependent)

   Available since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

10. pCAG-VSVM

    Plasmid

    #64086

    Purpose

    Expression vector for Vesicular Stomatitis Virus Indiana strain matrix gene

    Depositor

    Ian Wickersham

    Insert

    VSV matrix protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR_SFFV_LaG17_synNotch_TetRVP64

    Plasmid

    #79128

    Purpose

    Lentiviral vector for constitutive expression of the LaG17 GFP nanobody synNotch TetRVP64 Receptor (SFFV promoter)

    Depositor

    Wendell Lim

    Article

    Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

    Insert

    LaG17 GFP nanobody synNotch TetRVP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-JNK1

    Plasmid

    #86830

    Purpose

    mammalian expression of GFP-JNK1

    Depositor

    Rony Seger

    Article

    Zehorai et al Mol Cell Biol. 2014 Jan;34(2):259-70. do

    Insert

    JNK1 (MAPK8 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-mDlx-GFP-Fishell-1 (AAV9)

    Viral Prep

    #83900-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-mDlx-GFP-Fishell-1 (#83900). In addition to the viral particles, you will also receive purified pAAV-mDlx-GFP-Fishell-1 plasmid DNA. mDlx-driven expression of GFP in GABA-ergic interneurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Gordon Fishell

    Article

    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

    Promoter

    mDlx

    Tags

    GFP

    Available since

    Nov. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

14. PB-PARP1-Apex2–eGFP

    Plasmid

    #211555

    Purpose

    A piggyBac vector containing CMV-PARP1-Apex2-eGFP-IRES-Neo cassette.

    Depositor

    Chris Lord

    Article

    Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

    Insert

    PARP1 (PARP1 Human)
    

    Use

    Piggybac

    Tags

    Apex2-eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-EGxxFP-Cetn1

    Plasmid

    #50717

    Purpose

    Cetn1 genomic region was placed in pCAG-EGxxFP. Positive control for DSB mediated EGFP reconstitution.

    Depositor

    Masahito Ikawa

    Article

    Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

    Insert

    Cetn1 (Cetn1 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMH-SFB-DYRK1A

    Plasmid

    #101770

    Purpose

    Mammalian expression of DYRK1A fusion protein

    Depositor

    Michael Huen

    Article

    Li et al Nucleic Acids Res. 2024 Jul 5:gkae574. d

    Insert

    DYRK1A (DYRK1A Human)
    

    Use

    Tags

    S peptide-flag-streptavidin binding peptide (SFB)

    Expression

    Mammalian

    Mutation

    siRNA-resistant allele

    Promoter

    Available since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. LCC-ICCG_pET21b

    Plasmid

    #215408

    Purpose

    Expression construct for LCC-ICCG gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    LCC-ICCG
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    F243I/D238C/S283C/Y127G

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQE30-His-RF3

    Plasmid

    #124131

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Release factor 3 (prfC Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pY2ShHELIX_sgRNA_entry (CJT30)

    Plasmid

    #181781

    Purpose

    Expresses Y2-ShHELIX containing a nicking Y2 I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    Y2-nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y2-nAniI = F13Y, S111Y, K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDECKO_Malat1_A

    Plasmid

    #72620

    Purpose

    Expresses two gRNAs targeting MALAT1 promoter

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

    Insert

    gRNAs toward Malat1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (gRNA1) and H1 (gRNA2)

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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