We narrowed to 23,671 results for: crispr

1. pHAGE-IRES-puro-NLS-NLS-dPba3Cas13b-3xGFP-NLS

   Plasmid

   #191369

   Purpose

   Overexpression of NLS-NLS-dPba3Cas13b-3xGFP-NLS in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

   Insert

   NLS-NLS-dPba3Cas13b-3xGFP-NLS
   

   Tags

   3xGFP

   Expression

   Mammalian

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pXGFPC-5 Pxyl-dcas9 (Sth3)

   Plasmid

   #133317

   Purpose

   expresses dcas9 (Streptococcus thermophilus #3); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xyl locus in Caulobacter crescentus; tetracycline resistance

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   dcas9 (Streptococcus thermophilus #3)
   

   Use

   Unspecified

   Promoter

   xylose

   Available Since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCK364.306

   Plasmid

   #192642

   Purpose

   Expresses dSpRY and MCP-SoxS(R93A/S101A) and scRNA J306 on p15A-CmR

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

   Insert

   J306 scRNA
   

   Use

   CRISPR and Synthetic Biology

   Promoter

   BBa_J23119

   Available Since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHACK(Gal4)-DONR(T2A-Cas9)

   Plasmid

   #194768

   Purpose

   To insert T2A-Cas9 into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-Cas9 transgene.

   Depositor

   Chun Han

   Article

   Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

   Insert

   T2A-Cas9
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. qTAG-AAVS1-Ef1a-Zeo

   Plasmid

   #227268

   Purpose

   Empty AAVS1 targeting donor for the insertion of Zeo and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-nucGFP11-3xControlgRNA

   Plasmid

   #224568

   Purpose

   Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   GFP11-H2B
   

   Use

   CRISPR

   Tags

   Histone H2B (nuclear localization)

   Mutation

   Codon optimized

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPbuCas13b

   Plasmid

   #184565

   Purpose

   Inducible expression of PbuCas13b nuclease in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   Cas13b nuclease from Prevotella buccae
   

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAC1821_pCR8-SMN2-DN-RG6-SA

   Plasmid

   #118655

   Purpose

   Transient transfection; Expressed DR-SMN2-DN-RG6-SA-DR CasRx polycistronic gRNA with 3 spacers targeting SMN2 intron and 1 spacer targeting RG6 splice acceptor; Gateway Donor

   Depositor

   Albert Cheng

   Article

   Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

   Insert

   SMN2-DN-RG6-SA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBbdCas9S_P0526-sgRNAmreB

   Plasmid

   #149654

   Purpose

   all-in-one CRISPRi vector for targeting B. burgdorferi mreB

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   dCas9, lacI, sgRNAmreB
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   PflaB, PpQE30, P0526

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE-IRES-puro-NLS-NLS-dHgm4Cas13b-3xGFP-NLS

    Plasmid

    #191368

    Purpose

    Overexpression of NLS-NLS-dHgm4Cas13b-3xGFP-NLS in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

    Insert

    NLS-NLS-dHgm4Cas13b-3xGFP-NLS
    

    Tags

    3xGFP

    Expression

    Mammalian

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAC-crRNA-Km

    Plasmid

    #158712

    Purpose

    Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNA

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

    Insert

    AcGFP (for crRNA cloning)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    TetO

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. LentiU6-hMYOD1 gRNA_1-MS2-Puro

    Plasmid

    #192673

    Purpose

    Lentiviral expression of sgRNA targeting hMYOD1 promoter to activate human MYOD1 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Human MYOD1 activating gRNA #1 (MYOD1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-AaCas12b-2AeGFP

    Plasmid

    #121945

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AaCas12b
    

    Tags

    3xHA tag

    Expression

    Mammalian

    Available Since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC-hU6-crScaffold_SV40-BFP

    Plasmid

    #224860

    Purpose

    crRNA scaffold for RfxCas13d expressed from hU6 promoter and reporter BFP protein expressed from SV40 promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pN-PITCh-FAID

    Plasmid

    #127885

    Purpose

    Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter FAID tagging

    Depositor

    Peter Kaiser

    Article

    Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

    Insert

    GFP-Puro-H3F-AID (PPP2CA Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCJH002_10xHis-MBP-TEVcs-Cas12c(4)_Amp

    Plasmid

    #183069

    Purpose

    Bacterial protein expression plasmid of wild-type Cas12c_4. This is a R965H version of the Cas12c in Harrington et al., 2020.

    Depositor

    Jennifer Doudna

    Article

    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

    Insert

    Cas12c_4
    

    Use

    CRISPR

    Tags

    His10 and MBP

    Expression

    Bacterial

    Mutation

    Wild-type

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_ctrA

    Plasmid

    #133339

    Purpose

    for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    sgRNA_ctrA
    

    Use

    CRISPR

    Promoter

    constitutive

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE-BFP

    Plasmid

    #106282

    Purpose

    A version of the pHAGE vector with BFP and dsRed expressed separated by an IRES. Note that dsRed is not expressed.

    Depositor

    Jay Shendure

    Article

    Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460

    Insert

    CMV-BFP-IRES-DSRED(NOT-EXPRESSED)-WPRE-MCS
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-FLEXfrt-SaCas9-U6-sgSlc17a8

    Plasmid

    #124861

    Purpose

    Mutagenesis of Slc17a8

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc17a8 (Slc17a8 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYZ145

    Plasmid

    #98405

    Purpose

    Donor DNA plasmid to introduce leu1 deletion in S. pombe. Linearization with Not1 digestion.

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Inserts

    upstream homologous region to delete leu1 in genome by recombination (apc2 Fission Yeast)
    downstream homologous region to delete leu1 in genome by recombination (top2 Fission Yeast)
    

    Use

    CRISPR and Synthetic Biology

    Available Since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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