We narrowed to 23,148 results for: REP

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pT2h70CRET(#325)

Plasmid

#184060

Purpose

cyclofen-inducible CRE activation in zebrafish transient transgenic

Depositor

Sophie Vriz

Article

Feng et al Sci Rep. 2017 Aug 23;7(1):9195. doi: 10.

Insert

CRE-ERT2

Use

Zebrafish transient transgenesis

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Zf-Hsp70-4

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pTL464

Plasmid

#69881

Purpose

a dCirl transcriptional reporter allele that contains an optimized gal4.2::p65 cassette at the start codon of the genomic dCirl ORF

Depositor

Robert Kittel, Tobias Langenhan

Article

Scholz et al Cell Rep. 2015 May 12;11(6):866-74. doi:

Insert

CIRL (Cirl Fly)

Use

Phic31-integration vector

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiguide-puro_hNKD2sg#3

Plasmid

#174325

Purpose

gRNA targeting hNKD2 - encodes puromycin resistance

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

human NKD2

Use

Lentiviral

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiguide-puro_hNKD2sg#2

Plasmid

#174324

Purpose

gRNA targeting hNKD2 - encodes puromycin resistance

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

human NKD2

Use

Lentiviral

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiguide-puro_hNKD2sg#1

Plasmid

#174323

Purpose

gRNA targeting hNKD2 - encodes puromycin resistance

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

human NKD2

Use

Lentiviral

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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EC0_4_VPR

Plasmid

#163716

Purpose

Plasmid encoding VPR (VP64 - SV40 NLS - p65 - Rta) as a Type 4a part to be used in the Dueber YTK system (MoClo kit)

Depositor

Yvonne Nygård

Article

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Insert

VPR

Use

Synthetic Biology; Moclo vector level 0 (type 4a)

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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EC2_2_dCas9_VP64_sgRNA

Plasmid

#163707

Purpose

Yeast low copy plasmid with dCas9, sgRNA expression cassette and VP64 activation domain

Depositor

Yvonne Nygård

Article

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Inserts

dCas9
VP64+SV40 NLS

Expression

Yeast

Mutation

Point mutations D10A and H840A

Promoter

ScTEF1

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_hNKD2

Plasmid

#174329

Purpose

encodes human NKD2 cDNA with a C-terminal MycHis Tag.

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)

Tags

MycHis

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pGal4BD 1-147

Plasmid

#165819

Purpose

1-147 AA of GAL4 DNA binding domain, requires assembly with TL2-TL4 linker and TL5 activation domain

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Gal4BD 1-147

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pGAL4AD 768-881

Plasmid

#165820

Purpose

768-881 AA of GAL4 activation domain, requires assembly with TL2-TL4 linker and TL1 DNA binding domain

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

GAL4AD 768-881

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pET11a-N-DiB2

Plasmid

#168472

Purpose

N-fragment of the DiB2‐split protein. This plasmid needs to be co-transformed with Plasmid 168257: pMRBad-C-wtBlc to obtain DiB2‐split protein

Depositor

Jens Meiler

Article

Bozhanova et al Sci Rep. 2020 Jul 6;10(1):11049. doi: 10

Insert

N-fragment of DiB2

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP_A893E-talin1 (1-2541)

Plasmid

#166116

Purpose

For expression of full-length mouse talin-1 carrying mutation A893E in mammalian cells. Contains C-terminal EGFP tag.

Depositor

Vesa Hytönen

Article

Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

Insert

A893E-Talin-1 (Tln1 Mouse)

Use

Cmv promoter

Tags

EGFP

Expression

Mammalian

Mutation

A893E

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP_L1539P-talin1 (1-2541)

Plasmid

#166119

Purpose

For expression of full-length mouse talin-1 carrying mutation L1539P in mammalian cells. Contains C-terminal EGFP tag.

Depositor

Vesa Hytönen

Article

Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

Insert

L1539P-Talin-1 (Tln1 Mouse)

Use

Cmv promoter

Tags

EGFP

Expression

Mammalian

Mutation

L1539P

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP_L2509P-talin1 (1-2541)

Plasmid

#166123

Purpose

For expression of full-length mouse talin-1 carrying mutation L2509P in mammalian cells. Contains C-terminal EGFP tag.

Depositor

Vesa Hytönen

Article

Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

Insert

L2509P-Talin-1 (Tln1 Mouse)

Use

Cmv promoter

Tags

EGFP

Expression

Mammalian

Mutation

L2509P

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP_S1750F-talin1 (1-2541)

Plasmid

#166120

Purpose

For expression of full-length mouse talin-1 carrying mutation S1750F in mammalian cells. Contains C-terminal EGFP tag.

Depositor

Vesa Hytönen

Article

Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

Insert

S1750F-Talin-1 (Tln1 Mouse)

Use

Cmv promoter

Tags

EGFP

Expression

Mammalian

Mutation

S1750F

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP_D2086V-talin1 (1-2541)

Plasmid

#166122

Purpose

For expression of full-length mouse talin-1 carrying mutation D2086V in mammalian cells. Contains C-terminal EGFP tag.

Depositor

Vesa Hytönen

Article

Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

Insert

D2086V-Talin-1 (Tln1 Mouse)

Use

Cmv promoter

Tags

EGFP

Expression

Mammalian

Mutation

D2086V

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP_R1368W-talin1 (1-2541)

Plasmid

#166117

Purpose

For expression of full-length mouse talin-1 carrying mutation R1368W in mammalian cells. Contains C-terminal EGFP tag.

Depositor

Vesa Hytönen

Article

Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

Insert

R1368W-talin1-Talin-1 (Tln1 Mouse)

Use

Cmv promoter

Tags

EGFP

Expression

Mammalian

Mutation

R1368W

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP_Y1389C-talin1 (1-2541)

Plasmid

#166118

Purpose

For expression of full-length mouse talin-1 carrying mutation Y1389C in mammalian cells. Contains C-terminal EGFP tag.

Depositor

Vesa Hytönen

Article

Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

Insert

Y1389C-Talin-1 (Tln1 Mouse)

Use

Cmv promoter

Tags

EGFP

Expression

Mammalian

Mutation

Y1389C

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pJJF152_Phsp_Cas9

Plasmid

#163862

Purpose

heat shock-induced expression of Cas9 in C. elegans

Depositor

Nikolaus Rajewsky

Article

Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

Insert

Cas9, C. elegans codon optimized with intron and SV40_NLS

Use

CRISPR

Expression

Worm

Promoter

Phsp-16.48

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-Miro1 (412-618)

Plasmid

#127628

Purpose

GTPase2 domain of human Miro1 including transmembrane domain and C-terminal amino acids

Depositor

Josef Kittler

Article

Covill-Cooke et al EMBO Rep. 2020 Feb 5;21(2):e49865. doi:

Insert

Miro1 (RHOT1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

412-618 only

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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