We narrowed to 1,247 results for: Ncl
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Plasmid#134985PurposeThe plasmid includes a miRFP703 gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vectorDepositorInsertmiRFP703
UseLentiviralPromoterminCMVAvailable SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
Gata3-T2A-H2B-eGFP donor
Plasmid#113119PurposeDonor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Gata3 coding sequenceDepositorInsertGata3 donor region including homology arms and T2A-H2B-eGFP
UseSynthetic BiologyAvailable SinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
Fgfr2-T2A-H2B-Halo donor
Plasmid#113117PurposeDonor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Fgfr2 coding sequenceDepositorInsertFgfr2 donor region including homology arms and T2A-H2B-Halo
UseSynthetic BiologyAvailable SinceAug. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
EGFP-P2A-APEX2-csChBP
Plasmid#108877PurposeExpression of APEX2 fused to a conditionally stable nanobody against mCherry (includes green cytoplasmic reporter)DepositorInsertEGFP-P2A-APEX2-csChBP
ExpressionMammalianAvailable SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCCl-MCS_miniCMV_TagBFP
Plasmid#134986PurposeThe plasmid includes a TagBFP gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vectorDepositorInsertTagBFP
UseLentiviralPromoterminCMVAvailable SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
lentiMutB3GNT5-blast
Plasmid#208380Purposelentiviral vector for expressing human B3GNT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequenceDepositorInsertBeta-1,3-N-Acetylglucosaminyltransferase 5 (B3GNT5 Human)
UseLentiviralTagsmyc, FLAGExpressionMammalianMutationnt 922 c->g (PAM site inactivation)PromoterEF-1 alpha core promoterAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCas1_Cas2/3
Plasmid#89240PurposePlasmid contains the genes Cas1 and Cas2/3, cloned from Pseudomonas aeruginosa as a polycistronic operon. Cas1 includes an N-terminal Strep II tag and TEV protease cleavage site on Cas1DepositorInsertCas1 and Cas2/3
TagsStrep II tag on Cas1Available SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCK255
Plasmid#171155PurposeExpression of I-SceI gene for genome engineering in Pseudomonas with sacB gene included for counterselection with sucrose on pBBR1-GmR plasmidDepositorInsertI-SceI_sacB
ExpressionBacterialAvailable SinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTYF-sGFAP-IRES-tdTomato
Plasmid#166920PurposeEmpty plasmid backbone; includes IRES-tdTomato and sGFAP promoterDepositorTypeEmpty backboneUseLentiviralTagstdTomatoExpressionMammalianPromotersGFAPAvailable SinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
UNC50-myc
Plasmid#104382PurposeExpresses UNC50-myc wild-type in mammalian cellsDepositorAvailable SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
BcLOV-mCh-EGFR
Plasmid#208274PurposeExpresses BcLOV-EGFR for optogenetic activation of EGFR signaling. Includes an mCherry tag.DepositorInsertBcLOV-mCherry-EGFR
UseLentiviralTagsmCherryPromoterpCMVAvailable SinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-PHLPP1(N-terminal truncation)-deltaPH
Plasmid#22930PurposeMammalian expression of a HA tagged, N-terminal truncation of human PHLPP1 containing a deletion of the PH domainDepositorInsertPHLPP1-deltaPH (PHLPP1 Human)
TagsHAExpressionMammalianMutationIncludes 127-1205 aa. Deleted amino-terminal PH d…PromoterCMVAvailable SinceFeb. 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCB11
Plasmid#201111PurposepSR58.6 (Addgene plasmid #63176) modified to include the tetA gene upstream of sfGFP.DepositorInsertsCcaR
tetA(C)
sfGFP
ExpressionBacterialMutationC-to-T transition at pos. no. 1049 of tetA(C) lea…PromoterJ23100 and PcpcG2-172Available SinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-EEA-g1-PGK-Puro
Plasmid#102908PurposePiggyBac transposon system construct for U6 promoter-driven expression of a gRNA targeting EEA-motif. Includes PGK-puro selection cassette.DepositorInsertEEA-guide1-PGK-Puro
UseCRISPRExpressionMammalianPromoterU6Available SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTS2356_Tier3(PB)-PuroR
Plasmid#169655PurposeTier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-PuroR-pA-3'ITR)DepositorInsertPRPBSA-driven PuroR expression
ExpressionMammalianPromoterPRPBSAAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21c-IF3mt
Plasmid#31078DepositorInsertMature form human mitochondrial translational initiation factor 3 (MTIF3 Human)
TagsHisExpressionBacterialMutationIncludes coding sequence for the mature form. Ami…Available SinceAug. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPEE17
Plasmid#128361PurposeDetecting gene essentiality in Cryptococcus neoformans. Targets DNA constructs to the Safe Haven Site 1, a small gene-free region. Contains amdS2 counterselectable marker. This version of the Safe Haven vector includes direct repeats, enabling the formation of popouts on fluoroacetamide.DepositorInsertAcetamidase (amdS)
UseUnspecifiedPromoterTEF1Available SinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDH3
Plasmid#83789PurposeUseful for tagging yeast proteins with C-terminal CFP tag; G418 selectionDepositorInsertCFP
TagsCFPExpressionYeastMutationF64L, Y66W, N146I, M153T, V163A, N164H mutations …Available SinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
BcLOV-mCh-SOS
Plasmid#208279PurposeExpresses BcLOV-SOS for optogenetic activation of Ras/Erk signaling. Includes an mCherry tag.DepositorInsertBcLOV-mCh-SOS
UseLentiviralPromoterSFFVAvailable SinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLLX13
Plasmid#79825PurposeYeast and Bacterial shuttle vector, includes origin of transfer for conjugation, used for making a DNA capture vectorDepositorTypeEmpty backboneUseYeast and bacteria shuttle vector, contains origi…Available SinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only