We narrowed to 1,333 results for: Ncl

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pLY106

Plasmid

#130941

Purpose

Engineered sgRNA-LEA4 (2 BoxB aptamers) generator circuit including promoter Plux2

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEA4

Use

Synthetic Biology

Expression

Bacterial

Promoter

Plux2

Available Since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pBS_KS_attB2_SA(2)_T2AGeneSwitch

Plasmid

#125216

Purpose

Artificial phase 2 exon to include a spliced T2A-GeneSwitch effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

Depositor

Herman Dierick

Article

Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

Insert

GeneSwitch

Use

Other

Tags

T2A

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pBS_KS_attB2_SA(2)_T2AGAL80

Plasmid

#125219

Purpose

Artificial phase 2 exon to include a spliced T2A-GAL80 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

Depositor

Herman Dierick

Article

Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

Insert

GAL80

Use

Other

Tags

T2A

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBS_KS_attB2_SA(1)_T2AGeneSwitch

Plasmid

#125215

Purpose

Artificial phase 1 exon to include a spliced T2A-GeneSwitch effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

Depositor

Herman Dierick

Article

Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

Insert

GeneSwitch

Use

Other

Tags

T2A

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMDR1-4

Plasmid

#69485

Purpose

Deletion Construct

Depositor

Kathleen Scotto

Article

Jin et al Mol Cell Biol. 1998 Jul;18(7):4377-84.

Insert

MDR1 promoter (ABCB1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Insert includes MDR1 promoter sequence from -4 to…

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only
GLRX3

Plasmid

#39036

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

GLRX3 (GLRX3 Human)

Tags

His Tev

Expression

Bacterial

Mutation

A PCR artifact adds a C-terminal sequence includi…

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pLG-deltaA

Plasmid

#33151

Depositor

Michael Rosbash

Article

Legrain et al Cell. 1989 May 19;57(4):573-83.

Insert

RP51A-synthetic minimal intron (RPS17A Budding Yeast)

Tags

LacZ

Expression

Yeast

Mutation

The RP51A sequence was modified by digestion with…

Available Since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
pFloxin-Ofd1-Myc-S437R

Plasmid

#24577

Depositor

Jeremy Reiter

Article

Singla et al Dev Cell. 2010 Mar 16. 18(3):410-24.

Insert

Ofd1 (Ofd1 Mouse)

Use

Cre/Lox and Mouse Targeting

Tags

Myc

Mutation

Serine 437 changed to Arginine (codon AGT to CGT)…

Available Since

May 27, 2010

Availability

Academic Institutions and Nonprofits only

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p077 pCS xTRPS1 (M)

Plasmid

#11047

Depositor

Ramesh Shivdasani

Article

Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

Insert

TRPS1 mut (trps1 Frog)

Use

Mammalian, avian, and zebrafish

Mutation

Two Cys residues in the GATA-type zinc finger wer…

Available Since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only
Fus-BcLOV-mCh-SOS

Plasmid

#208275

Purpose

Expresses Fus-BcLOV-SOS for optogenetic activation of Ras/Erk signaling. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Article

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

Fus-BcLOV-mCh-SOS

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pRDV5 containing Sb-loop

Plasmid

#132695

Purpose

pRDV5 plasmid containing a loop Sybody for the generation of a qPCR standard curve

Depositor

Markus Seeger

Article

Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

Insert

Sb_loop including 5' and 3' flanking sequences used for ribosome display

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLX-TRV2

Plasmid

#180516

Purpose

Agrobacterium tumefaciens-based expression of TRV2 including a PEBV heterologous promoter and a polylinker

Depositor

Jose-Antonio Daros

Article

Aragones et al Biotechnol J. 2022 Mar 24:e2100504. doi:

Insert

Tobacco rattle virus RNA2 with a PEBV heterologous promoter and a polylinker

Expression

Plant

Promoter

35S CaMV

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCI SMN2

Plasmid

#72287

Purpose

SMN2 exon 7 splicing cassette

Depositor

Elliot Androphy

Article

Lorson et al Proc Natl Acad Sci U S A. 1999 May 25;96

Insert

SMN2 (SMN2 Human)

Expression

Mammalian

Mutation

contains exons 6-7-8 including introns, multiple …

Promoter

CMV

Available Since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pSG5-SUV39H2-FL

Plasmid

#89135

Purpose

mammalian expression of full-length 410 amino acid SUV39H2 histone methyltransferase from pSG5, includes 5' and 3' noncoding regions

Depositor

Elizabeth Wilson

Article

Askew et al Mol Cell Endocrinol. 2017 Mar 5;443:42-5

Insert

SUV39H2 (SUV39H2 Human)

Expression

Mammalian

Promoter

SV40

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCCl-MCS_miniCMV_EGFP

Plasmid

#134984

Purpose

The plasmid includes a EGFP gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vector

Depositor

Claudio Mussolino

Article

Roman Azcona et al Nat Protoc. 2020 Oct;15(10):3410-3440. d

Insert

EGFP

Use

Lentiviral

Promoter

minCMV

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQAT51

Plasmid

#223423

Purpose

T-DNA vector for LbCas12a-RRV based mutagenesis for dicot plants; highly efficient including TTV PAM; LbCas12a-RRV and the crRNA were driven by separate 2x35s promoter; Kanamycin for plants selection.

Depositor

Yiping Qi

Article

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

2x35s-LbCas12a-RRV-2x35s-crRNA scaffold

Use

CRISPR

Tags

NLS

Expression

Plant

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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JD690

Plasmid

#160397

Purpose

Binary vector pGWB14 including citrus GRF4-GIF1 under 35S promoter/ Plant transformation

Depositor

Jorge Dubcovsky

Article

Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

Insert

35S::citrus GRF4-GIF1 chimera

Promoter

35S

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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YFP-CAAX

Plasmid

#223558

Purpose

Plasmid expressing YFP(Venus) followed by the last 25 amino acids of human KRas4b including the polybasic regions and CAAX prenylation box

Depositor

Mikel Garcia-Marcos

Article

Luebbers et al Sci Signal. 2025 Mar 25;18(879):eadp6457

Insert

YFP(Venus) - KRas4b

Expression

Mammalian

Available Since

March 28, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pR26 GFP Dest

Plasmid

#74283

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a loxP flanked stop cassette and GFP, for gateway cloning

Depositor

Ralf Kuehn

Article

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pTipi2.1-AZU_SP

Plasmid

#229144

Purpose

Allows for expression of recombinant antibodies. Includes human azurocidin signal peptide.

Depositor

Institute for Protein Innovation (IPI)

Article

Yang et al bioRxiv, March 20, 2026

Type

Empty backbone

Expression

Mammalian

Available Since

Jan. 9, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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