-
Plasmid#222578PurposePiggyBac transposon plasmid for doxycycline inducible expression of DPPA3DepositorInsertDPPA3 (DPPA3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-CTCFL
Plasmid#222546PurposePiggyBac transposon plasmid for doxycycline inducible expression of CTCFLDepositorInsertCTCFL (CTCFL Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-MBP_F
Plasmid#146318PurposeMammalian Expression of MBPDepositorInsertMBP (MBP Human)
UseTagsExpressionMammalianMutationN217SPromoterAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pABpuro-BluF
Plasmid#46824Purposeluciferase reporter for BmalI promoterDepositorInsertBmal1 (Bmal1 Mouse)
UseLentiviral and LuciferaseTagsluciferaseExpressionMammalianMutationPromoterBmal1Available sinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
PB-PE
Plasmid#162796PurposeAll-in-one prime editor piggyBac transposonDepositorInsertSpCas9_ H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
UseCRISPR and Synthetic Biology; Piggybac transposonTagsSV40 NLSExpressionMammalianMutationPromoterCAGAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-PLXNB2
Plasmid#86237PurposeLentivirus for expression of human Plexin-B2DepositorInsertPlexin-B2 (PLXNB2 Human)
UseLentiviralTagsExpressionMammalianMutationfull length PLXNB2, CRISPR resistantPromoterhuman PGK promoterAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cox1-FusXTBE-RightTALE
Plasmid#196503PurposeExpresses right-side TALE–DddAtox C term half cox1-FusXTBE in zebrafishDepositorInsertIDH2 MTS-cox1 right TALE-G1397 DddA-C-UGI-SV40 polyA
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cox3-FusXTBE-RightTALE
Plasmid#196505PurposeExpresses right-side TALE–DddAtox C term half cox3-FusXTBE in zebrafishDepositorInsertIDH2 MTS-cox3 right TALE-G1397 DddA-C-UGI-SV40 polyA
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_mCherry_KLF4
Plasmid#140104PurposeCRISPR-Cas9 library validationDepositorInsertgRNA1+scaffold+H1promoter+gRNA2
UseLentiviralTagsExpressionMutationPromotergRNA1 under U6 and gRNA2 under H1Available sinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOUPc-Rh159-GFP
Plasmid#179280PurposeAll in one "tet-on" lentivirus vector that expresses rhesus cytomegalovirus Rh159 fused to an eGFP tag at its cytoplasmic tail. Rh159 is an ER resident protein that binds NKG2D activating ligands.DepositorInsertRh159 fused to eGFP
UseLentiviral; All-in-one "tet" on lentivi…TagseGFPExpressionMammalianMutationPromoterTet Responsive Element 3GAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_hMed25
Plasmid#206958PurposeThe ORF of human Med25 cDNA amplified from the RT human liver RNA was cloned into pGEMTE vector and then subclone at XhoI/XbaI sites of the pcDNA3.2(+), the mammalian expression vector.DepositorInsertHomo sapiens mediator complex subunit 25 (MED25), transcript variant 1, mRNA (MED25 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUCM-AAVS1-TO-hNGN2
Plasmid#105840PurposeDonor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuronDepositorInsertsUseCRISPR and TALENTagsExpressionMutationPromoterCAG, EF-1alpha, and TRE3GAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETM33_TNKS_TANK-peptide-bind
Plasmid#178489PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertTNKS_TANK-peptide-bind (TNKS Synthetic, Human)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM33_MAD2L1_HORMA
Plasmid#178480PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertMAD2L1_HORMA (MAD2L1 Synthetic, Human)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
T-STOP sgRNA-1
Plasmid#83346PurposeThe sgRNA at the stop codon of human Brachyury(T) locusDepositorInsertT-STOP sgRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETM33_KEAP1_KELCH
Plasmid#178496PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertKEAP1_KELCH (KEAP1 Synthetic, Human)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM41_KEAP1_KELCH
Plasmid#178476PurposeBacterial expression of human domain with His-tag and MBP tagDepositorInsertKEAP1_KELCH (KEAP1 Synthetic, Human)
UseTagsHis-MBPExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM33_CRK_SH3
Plasmid#178472PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertCRK_SH3 (CRK Synthetic, Human)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM33_IRF3_SMAD/FHA
Plasmid#178479PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertIRF3_IRF3 (IRF3 Synthetic, Human)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
YopH (residues 164-468)
Plasmid#79749PurposeThis plasmid encodes the active catalytic domain (amino acid residues 164-468) of YopH. Inteded for co-expression with human Tyr kinase plasmids to enhance bacterial kinase expression.DepositorInsertYopH (residues 164-468)
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only