We narrowed to 43,334 results for: lars

1. pNoROS-CAT

   Plasmid

   #170152

   Purpose

   Mammalian expression vector encoding a constitutive nucleolar targeted roGFP and a doxycycline inducible nucleolar targeted catalase. Used to study nucleolar oxidation

   Depositor

   Dimitri Pestov

   Article

   Sapio et al Front Mol Biosci. 2021 Apr 26;8:678488.

   Inserts

   catalase (Cat Mouse)
   roGFP2
   

   Use

   Genome integration via sleeping beauty transposon…

   Tags

   FLAG tag and Nucleolar Localization Sequence (NoL…

   Expression

   Mammalian

   Mutation

   Promoter

   RPBSA and TRE promoter

   Available since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEN313 - Rad21-Halo-Frt-PGK-EM7-NeoR-bpA-Frt targeting

   Plasmid

   #156431

   Purpose

   Targeting vector to introduce an Halotag cassette at the mouse Rad21 locus using NEOMYCINE selection. Designed for using with sgRNA CCACGGTTCCATATTATCTG

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   RAD21, Halotag (Rad21 Mouse)
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Available since

   Sept. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEN487 - Sororin-AID[71-114]-eGFP-FRT-Blast-FRT targeting conuct

   Plasmid

   #156433

   Purpose

   Targeting vector to introduce an AID-eGFP cassette at the mouse Cdca5 (SORORIN) locus using BLASTICIDIN selection. Auxin-inducible degron system. Designed to be used with sgRNA GGGATGCCCGTCATTAAGTG

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   SORORIN, AID (Cdca5 Mouse)
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Available since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. SV40 1: pBSSVD2005

   Plasmid

   #21826

   Depositor

   David Ron

   Insert

   SV40 Large T (SV40gp6 simian virus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   likely expresses aa109-708 of the large T antigen

   Promoter

   Available since

   Sept. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAU003 - pTRE3G-Cterm_Nterm_switch_CTCF-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

   Plasmid

   #156430

   Purpose

   Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   CTCF, rtta3G, TetO3G (Ctcf Mouse)
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Cterm_Nterm_switched

   Promoter

   Available since

   Sept. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEN515 - pTRE3G-CTCF(ZFmut)-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

   Plasmid

   #156434

   Purpose

   Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA (all Zinc fingers point mutated), targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA.

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   CTCF, rtta3G, TetO3G (Ctcf Mouse)
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   All zinc fingers mutated

   Promoter

   Available since

   Sept. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAU002 - pTRE3G-CTCF(DELTA1-265)-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

   Plasmid

   #156429

   Purpose

   Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   CTCF, rtta3G, TetO3G (Ctcf Mouse)
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   DELTA1-265

   Promoter

   Available since

   Sept. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEN366 - pTRE3G-CTCF-mRuby2-BGHpA-CAGGS-rtta3G-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE donor

   Plasmid

   #156432

   Purpose

   Vector to introduce a constitutive rtta3G cassette and a DOX-inducible wild-type CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   CTCF, rtta3G, TetO3G (Ctcf Mouse)
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Available since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKC-Tol2

   Plasmid

   #85600

   Purpose

   Source of Tol2 transposase driven by a mini-CAGS promoter.

   Depositor

   Scott Fahrenkrug, Karl Clark

   Article

   Clark et al BMC Biotechnol. 2007 Jul 17;7:42.

   Insert

   Tol2 transposase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mini-CAG

   Available since

   Dec. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKUb-Tol2

    Plasmid

    #85601

    Purpose

    Source of Tol2 transposase driven by a human ubiquitin promoter.

    Depositor

    Scott Fahrenkrug, Karl Clark

    Article

    Clark et al BMC Biotechnol. 2007 Jul 17;7:42.

    Insert

    Tol2 transposase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human Ubiquitin

    Available since

    Feb. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKTol2C-EGFP

    Plasmid

    #85598

    Purpose

    Tol2 transposon with EGFP driven by minimal-CAGs promoter.

    Depositor

    Scott Fahrenkrug, Karl Clark

    Article

    Clark et al BMC Biotechnol. 2007 Jul 17;7:42.

    Insert

    Tol2 transposon with EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mini-CAG

    Available since

    Dec. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. GGE0029

    Plasmid

    #120730

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Destination vector for the insertion of the assembly

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Type

    Empty backbone

    Use

    Cloning/assembly plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKTol2P-PTK

    Plasmid

    #85599

    Purpose

    Tol2 transposon with a puromycin-thymidine kinase fusion driven by a PGK promoter.

    Depositor

    Scott Fahrenkrug, Karl Clark

    Article

    Clark et al BMC Biotechnol. 2007 Jul 17;7:42.

    Insert

    Tol2 transposon with a puromycin-thymidine kinase fusion
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Sept. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPTn2P-PTK

    Plasmid

    #85604

    Purpose

    Passport transposon with a puromycin-thymidine kinase fusion driven by a PGK promoter.

    Depositor

    Scott Fahrenkrug, Karl Clark

    Article

    Clark et al BMC Biotechnol. 2007 Jul 17;7:42.

    Insert

    Passport transposon with a puromycin-thymidine kinase fusion
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. GGE177

    Plasmid

    #120766

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for position 1 (T1_Xpr2)

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    T1_Xpr2
    

    Use

    Cloning/assembly plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. GGE085

    Plasmid

    #120738

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position1 (P1_Tef)

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    P1_Tef
    

    Use

    Cloning/assembly plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GGE002

    Plasmid

    #120739

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position1 (P1_GAPdh)

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    P1_GAPdh
    

    Use

    Cloning/assembly plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GGE001

    Plasmid

    #120740

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position1 (P1_PGM)

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    P1_PGM
    

    Use

    Cloning/assembly plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GGE080

    Plasmid

    #120774

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for position 3 (T3_Lip2)

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    T3_Lip2
    

    Use

    Cloning/assembly plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GGE014

    Plasmid

    #120765

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for position 1 (T1_Lip2)

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    T1_Lip2
    

    Use

    Cloning/assembly plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge