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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,344 results for: multi

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Showing: 721 - 740 of 18344 results

  3. pX330A_D10A-1x2

    Plasmid
    #58772
    Purpose
    Expresses Cas9 nickase and gRNA
    Depositor
    Takashi Yamamoto
    Publication
    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized S. pyogenes Cas9 (D10A) nickase
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    D10A nickase-converting mutation
    Promoter
    CBh
    Available since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    58772-plasmid-map-sequence-id-91082

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  7. pLenti-Tet-coBxb1-2A-BFP_IRES-iCasp9-2A-Blast_rtTA3

    Plasmid
    #171588
    Purpose
    Lentivector encoding a DNA recombinase landing pad with Bxb1 integrase and iCasp9 negative selection behind a Tet-inducible promoter
    Depositor
    Kenneth Matreyek
    Publication
    Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.
    Inserts
    Bxb1 integrase
    iCasp9
    BFP
    rtTA3G
    Use
    Lentiviral and Synthetic Biology
    Expression
    Mammalian
    Available since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    171588-plasmid-map-sequence-id-335078

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  8. Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A (Lentiviral Prep)

    Viral prep
    #92379-LV
    Purpose
    Ready-to-use Lentiviral Prep particles produced from Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A (#92379). In addition to the viral particles, you will also receive purified Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A plasmid DNA. Ready-to-use lentiviral pooled library for CRISPR activation screening in human cells. Contains 56,762 sgRNAs, targeting 18,885 genes. Concentrated lentiviral particles carrying set A of the Calabrese human CRISPRa sgRNA activation library in backbone XPR_502 (P65 HSF) .
    Depositor
    John Doench, David Root
    Publication
    Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:
    Available since
    Aug. 3, 2017
    Availability
    Academic Institutions and Nonprofits only

  19. pFUGW-FIRE-pHLy

    Plasmid
    #170774
    Purpose
    Ratiometric biosensor expressed with UbC promoter for probing lysosomal pH in mammalian cells
    Depositor
    Aimee Kao
    Publication
    Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse
    Insert
    LAMP1 (LAMP1 Human)
    Use
    Lentiviral
    Tags
    mCherry and mTFP1
    Expression
    Mammalian
    Mutation
    84 nucleotide Signal Sequence switched to the beg…
    Promoter
    hUbC
    Available since
    Sept. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    170774-plasmid-map-sequence-id-339008

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  20. pCBC-MT1T2

    Plasmid
    #50593
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites
    Depositor
    Qi-Jun Chen
    Publication
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Insert
    T1, gRNA scaffold, OsU3t plus, TaU3p, T2
    Use
    CRISPR; Pcr template
    Expression
    Plant
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    50593-plasmid-map-sequence-id-451910

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Showing: 721 - 740 of 18344 results