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pLL3.7-EF-EYFP-YAP1_R89A-PolyA

Plasmid

#112291

Purpose

Fluorescently labels human YAP1-2gamma isoform (504 aa) R89A mutant

Depositor

Erik Sahai

Article

Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

Insert

EYFP-YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

Human YAP1-2gamma with Arginine 89 to Alanine

Promoter

EF-1 alpha

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLL3.7-EF-EYFP-YAP1_L91A-PolyA

Plasmid

#112292

Purpose

Fluorescently labels human YAP1-2gamma isoform (504 aa) L91A mutant

Depositor

Erik Sahai

Article

Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

Insert

EYFP-YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

Human YAP1-2gamma with Leucine 91 to Alanine

Promoter

EF-1 alpha

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLL3.7-EF-EYFP-YAP1_F95A-PolyA

Plasmid

#112293

Purpose

Fluorescently labels human YAP1-2gamma isoform (504 aa) F95A mutant

Depositor

Erik Sahai

Article

Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

Insert

EYFP-YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

Human YAP1-2gamma with Phenylalanine 95 to Alanine

Promoter

EF-1 alpha

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-vsv-Incenp d543-746-GFP

Plasmid

#108503

Purpose

inducible expression of vsv-INCENP d543-746-EGFP

Depositor

Susanne Lens

Article

van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

Insert

INCENP d543-746 (INCENP Human)

Tags

EGFP and VSV

Expression

Mammalian

Mutation

delta alpha helix

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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CSK22_HUMAN_D0

Plasmid

#79704

Purpose

This plasmid encodes the kinase domain of CSK22. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

CSK22 (CSNK2A2 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
DGKA gRNA (BRDN0001144932)

Plasmid

#76608

Purpose

3rd generation lentiviral gRNA plasmid targeting human DGKA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DGKA (DGKA Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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DGKA gRNA (BRDN0001147297)

Plasmid

#76605

Purpose

3rd generation lentiviral gRNA plasmid targeting human DGKA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DGKA (DGKA Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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DGKA gRNA (BRDN0001148437)

Plasmid

#76606

Purpose

3rd generation lentiviral gRNA plasmid targeting human DGKA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DGKA (DGKA Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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DGKA gRNA (BRDN0001145658)

Plasmid

#76607

Purpose

3rd generation lentiviral gRNA plasmid targeting human DGKA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DGKA (DGKA Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pRRLHygro-pEF1a-p53ashL344A-mKate2-splitmVenusC

Plasmid

#69585

Purpose

Expresses mutated p53-L344A tagged with mKate2 and split C-term mVenus

Depositor

Galit Lahav

Article

Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

Insert

p53-L344A (TP53 Human)

Use

Lentiviral

Tags

mKate2 and split mVenus - C term

Expression

Mammalian

Mutation

p53 L344A mutation that prevents tetramerization …

Promoter

EF1alpha

Availability

Academic Institutions and Nonprofits only

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pSLPB2B-FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

Plasmid

#187951

Purpose

FKBP12 (F36V mutant) degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

Depositor

Edda Schulz

Article

Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

Insert

FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP

Use

CRISPR and Synthetic Biology

Tags

GGS linker

Expression

Mammalian

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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Ad:2CMV_A2R2-N25K3

Plasmid

#122242

Purpose

Expresses a dominant negative Kash construct that disrupts LINC complexes in addition to fluorescently tagged (mRuby2) α-Actinin-2 on an adenoviral backbone

Depositor

Corey Neu

Article

Seelbinder et al Nat Biomed Eng. 2021 Dec;5(12):1500-1516

Inserts

C-terminal end of Nesprin-3 (Syne3 Mouse)
Actinin alpha 2 (Actn2 Mouse)

Use

Adenoviral

Tags

Signal Peptide (TOR1A, NM_000113.2), mNeptune2.5,…

Expression

Mammalian

Promoter

CMV

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-iCreV

Plasmid

#140135

Purpose

Can be used to generate AAV virus that will express light-inducible site-specific iCreV recombinase

Depositor

Hongkui Zeng

Article

Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

Has Service

AAV PHP.eB and AAV1

Insert

iCreV

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1alpha

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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lenti.DTR.GFP

Plasmid

#201962

Purpose

Lentiviral vector that expresses diptheria toxin receptor (DTR) C-terminally fused to EGFP

Depositor

Bernhard Schmierer

Article

Fernandez Moro et al Nat Commun. 2023 Aug 18;14(1):5024. doi:

Insert

Diptheria Toxin Receptor

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1alpha

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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FAS_pLX307

Plasmid

#98334

Purpose

Lentiviral expression of FAS

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

FAS (FAS Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pEF6-Acly

Plasmid

#70765

Purpose

Expresses mouse ATP-citrate lyase in mammalian cells

Depositor

Kathryn Wellen

Article

Lee et al Cell Metab. 2014 Aug 5;20(2):306-19. doi

Insert

ATP Citrate Lyase (Acly Mouse)

Tags

myc-epitope

Expression

Mammalian

Promoter

EF1alpha

Available Since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pLViN-E-cadherin-GFP

Plasmid

#229710

Purpose

Lentiviral expression of E-cadherin-GFPTG in mammalian cells

Depositor

Alpha Yap

Article

Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

Insert

CDH1 E-CADHERIN Human (CDH1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-BAZ1A

Plasmid

#65371

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BAZ1A (BAZ1A Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

796I compared to all reference sequences

Promoter

CMV

Available Since

June 15, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-N-Flag-NLRP3 1-90

Plasmid

#75137

Purpose

Expresses N-terminal Flag-tagged amino acids 1-90 of NLRP3 (the pyrin domain) in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

NLR family, pyrin domain containing 3 (Nlrp3 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

fragment encoding amino acids 1-90

Promoter

CMV

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 hSCN5AdeltaSIV GFP Nter

Plasmid

#145770

Purpose

expresses human Nav1.5 lacking the SIV motif and tagged with GFP at its N-terminus in mammalian cells

Depositor

Hugues Abriel

Article

Petitprez et al Circ Res. 2011 Feb 4;108(3):294-304. doi

Insert

sodium voltage-gated channel alpha subunit 5 (SCN5A Human)

Tags

GFP

Expression

Mammalian

Mutation

Stop codon at Serine 2013

Promoter

CMV

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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