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BRAC/pcDNA3

Plasmid

#51967

Purpose

mammalian expression of BRAC, a BRET-based autofluorescent Ca2+ indicator

Depositor

Takeharu Nagai

Article

Saito et al PLoS One. 2010 Apr 1;5(4):e9935. doi: 10

Insert

BRAC

Expression

Mammalian

Available Since

March 18, 2014

Availability

Academic Institutions and Nonprofits only
pIRES.Puro.EGFP

Plasmid

#24176

Depositor

Robert Sobol

Article

Jelezcova et al Mutat Res. 2010 Apr 1;686(1-2):57-67. do

Insert

EGFP

Expression

Mammalian

Mutation

x

Available Since

April 9, 2010

Availability

Academic Institutions and Nonprofits only
pAAV_hSyn_WiChR_TS_mScarlet_ER

Plasmid

#205997

Purpose

High efficient, K+ selective channelrhodopsin for the inhibition of neurons

Depositor

Peter Hegemann, Johannes Vierock

Article

Vierock et al Sci Adv. 2022 Nov 16:eadd7729.

Has Service

AAV9

Insert

Wobblia Inhibitory Channelrhodopsin

Use

AAV

Tags

TS, mScarlet, ER

Promoter

hSyn

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FRT-Rev-TdTomato

Plasmid

#191203

Purpose

FlpO dependent TdTomato expression

Depositor

Janelia Viral Tools

Article

Winnubst et al Cell. 2019 Sep 19;179(1):268-281.e13. do

Insert

TdTomato

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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p246 eSpCas9_2gRNAs_mH11

Plasmid

#164854

Purpose

gRNA vector for targeting mouse H11 locus

Depositor

Philip Jordan

Article

Pryzhkova et al Stem Cell Res. 2020 Nov 5;49:102078. doi

Insert

gRNAs for targeting mouse H11 locus

Expression

Mammalian

Promoter

U6

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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p285 TIR1_HA_mH11

Plasmid

#164855

Purpose

constitutive TIR1 expression vector for mouse H11 locus

Depositor

Philip Jordan

Article

Pryzhkova et al Stem Cell Res. 2020 Nov 5;49:102078. doi

Insert

OsTIR1

Tags

HA tag

Expression

Mammalian

Promoter

EF1-alpha

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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PLX306 iCre tetO tomato rtTA

Plasmid

#158050

Purpose

lenti-viral construct with Cre recombinase and doxycycline inducible expression of tdTomato

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

tdTomato

Tags

none

Expression

Mammalian

Promoter

tetO promoter

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pRenilla

Plasmid

#118059

Purpose

GBPart - Renilla Luciferase CDS

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

Renilla

Use

Luciferase and Synthetic Biology; Gbpart

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pRSFDuet1-Cdc45

Plasmid

#126878

Purpose

Expresses human Cdc45 in bacteria

Depositor

Luca Pellegrini

Article

Simon et al Nat Commun. 2016 May 18;7:11638. doi: 10

Insert

CDC45 (CDC45 Human)

Tags

No tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pGTag-TagRFP-B-actin

Plasmid

#117808

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pEM705 Gs12

Plasmid

#109357

Purpose

GsX chimera with Gα12 C-terminus

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gs12 chimera

Tags

None

Expression

Mammalian

Promoter

CAG

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pECFP-Rac1Q61L

Plasmid

#105292

Purpose

Constitutively active Rac 1

Depositor

Kenneth Yamada

Article

Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

Insert

Rac1 (RAC1 Human)

Tags

CFP

Expression

Mammalian

Mutation

Q61L

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pEB1-mGFPmut3

Plasmid

#103981

Purpose

Plasmid encoding mGFPmut3

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mGFPmut3

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S65G, S72A, …

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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HA-Rab8a-DN (T22N)

Plasmid

#101049

Purpose

Expresses HA-tagged human Rab8-DN (T22N) in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

Insert

Rab8a (RAB8A Human)

Tags

HA

Expression

Mammalian

Mutation

T22N

Promoter

CMV

Available Since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pAG416GPD-Cerulean-YPT7

Plasmid

#18847

Depositor

Susan Lindquist

Article

Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

YPT7 (YPT7 Budding Yeast)

Tags

Cerulean

Expression

Yeast

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pENTR-QK1

Plasmid

#16183

Depositor

Huda Zoghbi

Article

Lim et al Cell. 2006 May 19. 125(4):801-14.

Insert

Quaking (QKI Human)

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-fDIO EYFP (AAV Retrograde)

Viral Prep

#55641-AAVrg

Purpose

Ready-to-use AAV Retrograde particles produced from pAAV-Ef1a-fDIO EYFP (#55641). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO EYFP plasmid DNA. EF1a-driven, Flp-dependent expression of EYFP. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Promoter

EF1a

Tags

EYFP (Flp-dependent)

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 VDAC1-TC

Plasmid

#211733

Purpose

Expresses tetracysteine tagged VDAC1 in mammalian cells. TC-tag is attached to the C-terminus of VDAC1.

Depositor

Roland Malli

Article

Pilic et al PLoS One. 2024 Oct 18;19(10):e0311107. d

Insert

Voltage-dependent anion channel 1 (VDAC1 )

Tags

Tetracysteine

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_T6B -mCherry

Plasmid

#205600

Purpose

expresses human T6B peptide fused with mChery in mammalian cells

Depositor

Marc Friedlander

Article

Sekar et al Nat Biotechnol. 2023 Sep 21. doi: 10.103

Insert

T6B peptide (fragment of human TRNC6B 599-683aa) (TNRC6B Human)

Tags

mCherry

Expression

Mammalian

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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NbV5-mCherry

Plasmid

#201474

Purpose

Indirect microscopy/imaging of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbV5-mCherry

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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