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pOTTC351 - pAAV CaMKIIa hChR2(H134R)-iRFP

Plasmid

#47905

Purpose

An AAV vector that expresses channel rhodopsin 2 (H134R) (fused to iRFP) under the CaM Kinase IIa promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

hChR2(H134R)

Use

AAV

Tags

iRFP

Expression

Mammalian

Mutation

H134R

Promoter

CaMKIIa

Available Since

Sept. 19, 2013

Availability

Academic Institutions and Nonprofits only

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LRRK2 gRNA (BRDN0001146754)

Plasmid

#77330

Purpose

3rd generation lentiviral gRNA plasmid targeting human LRRK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

LRRK2 (LRRK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CNTF-HA

Plasmid

#195517

Purpose

Expresses HA-tagged CNTF in mammalian cells

Depositor

Kevin Park

Article

Ribeiro et al Exp Neurol. 2022 Sep;355:114147. doi: 10

Insert

Ciliary neurotrophic factor (CNTF Human)

Use

AAV

Tags

HA-tag and NGF signal peptide

Expression

Mammalian

Promoter

UbC promoter with beta-globin intron

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pJEP311-pAAV-EFS-SaCas9-P2A-HAFLAGHA-KASH-pA

Plasmid

#113688

Purpose

SaCas9 driven by EFS. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

SaCas9 (NEWENTRY )

Use

AAV and CRISPR

Tags

Flag, HAx2, KASH, and NLS

Promoter

Cytomegalo Virus(CMV)

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pPHAGE-EF1α-Luc-C20orf24-3’UTR-WT

Plasmid

#128551

Purpose

Lentiviral constitutive expression of Firefly luciferase under control of WT 3'UTR of human C20orf24.

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

Firefly luciferase

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pJEP312-pAAV-CMV-SaCas9-P2A-HAFLAGHA-KASH-pA

Plasmid

#113689

Purpose

SaCas9 driven by CMV. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

SaCas9 (NEWENTRY )

Use

AAV and CRISPR

Tags

Flag, HAx2, KASH, and NLS

Promoter

Cytomegalo Virus(CMV)

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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LRRK2 gRNA (BRDN0001147881)

Plasmid

#77333

Purpose

3rd generation lentiviral gRNA plasmid targeting human LRRK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

LRRK2 (LRRK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PINK1 gRNA (BRDN0001144744)

Plasmid

#78038

Purpose

3rd generation lentiviral gRNA plasmid targeting human PINK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PINK1 (PINK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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W118-1_hRSK3(RPS6KA2)_K91A_K444A

Plasmid

#180385

Purpose

lentiviral transduction of human RSK3 (RPS6KA2) gene with point mutations (K91A and K444A) that inactivate kinase activity

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

RPS6KA2_K91A/K444A (RPS6KA2 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

K91A/K444A

Promoter

CMV

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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ERBB4 gRNA (BRDN0001162324)

Plasmid

#76198

Purpose

3rd generation lentiviral gRNA plasmid targeting human ERBB4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ERBB4 (ERBB4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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GAK gRNA (BRDN0001146687)

Plasmid

#76470

Purpose

3rd generation lentiviral gRNA plasmid targeting human GAK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GAK (GAK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pFB-EF1a-DIO-Rpl22l1-3xFLAG-2A-GFP

Plasmid

#245377

Purpose

Cre-dependent expression of FLAG-tagged Rpl22l1 and EGFP.

Depositor

D. James Surmeier

Article

Geibl et al Mol Neurodegener. 2024 Oct 8;19(1):69. d

Insert

Rpl22l1 (Rpl22l1 Mouse)

Use

AAV

Tags

3xFLAG, T2A-GFP

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-muGFP-hATG2A mLIR

Plasmid

#215506

Purpose

Expresses GFP tagged human ATG2A with the mutation in LC3 interacting region.

Depositor

Noboru Mizushima

Article

Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

Insert

Autophagy related 2A (ATG2A Human)

Use

Retroviral

Tags

muGFP

Expression

Mammalian

Mutation

P656R (natural variant with no functional relevan…

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgABI2-1

Plasmid

#210139

Purpose

knock out ABI2 in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Abl interactor 2 (ABI2 Human)

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgCYFIP1-1

Plasmid

#210137

Purpose

knock out CYFIP1 in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Cytoplasmic FMR1-interacting protein 1 (CYFIP1 Human)

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgABI2-2

Plasmid

#210140

Purpose

knock out ABI2 in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Abl interactor 2 (ABI2 Human)

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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Rufy3 scFv [N483/126]

Plasmid

#182089

Purpose

Mammalian Expression of Rufy3 scFv. Derived from hybridoma N483/126.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Rufy3 (Mus musculus) (Rufy3 Mouse)

Tags

Sortase, His

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pPHAGE-EF1α-Luc-C20orf24-3’UTR-VAR

Plasmid

#128507

Purpose

Lentiviral constitutive expression of Firefly luciferase under control of 3'UTR of human C20orf24 with variant from COX deficiency patient.

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

Firefly luciferase

Use

Lentiviral and Luciferase

Expression

Mammalian

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE-RSLID1(WT)

Plasmid

#122296

Purpose

expresses RSL1D1 protein in mammalian cells (puro resistance)

Depositor

Gerardo Ferbeyre

Article

Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

Insert

RSL1D1 (RSL1D1 Human)

Use

Retroviral

Expression

Mammalian

Promoter

vector's LTR

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pJEP324-pAAV-FullH1TO-SaCa9sgRNAi(emx1sg1)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA

Plasmid

#113701

Purpose

Dox-inducible H1 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1. Followed by an EFS driven GFP-KASH in a separate reading frame.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Inserts

SaCas9 gRNA Cassete
GFP

Use

AAV and CRISPR

Tags

KASH

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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