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We narrowed to 29,164 results for: eng

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Showing: 7401 - 7420 of 29164 results

  1. pDIRECT_22D

    Plasmid
    #91136
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:npt II
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
    Use
    CRISPR
    Expression
    Plant
    Mutation
    D10A, H840A
    Available since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    91136-plasmid-map-sequence-id-182475

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  4. lenti SYN-SVI-DIO-dCas9-VPR

    Plasmid
    #164576
    Purpose
    Expresses a SYN-driven, intron-containing dCas9-VPR fusion in which the part of the dCas9 cassette is double floxed and in inverted orientation (DIO). Requires Cre-dependent recombination.
    Depositor
    Jeremy Day
    Publication
    Carullo et al eNeuro. 2021 Jul 26. pii: ENEURO.0188-21
    Insert
    A DIO FLAG-dCas9-VPR cassette containing an SV40 intron
    Use
    CRISPR, Cre/Lox, and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Available since
    Feb. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    164576-plasmid-map-sequence-id-323657

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  6. Cenp-B INCENP GFP

    Plasmid
    #45238
    Purpose
    Target INCENP to centromeres via full length CENP-B
    Depositor
    Michael Lampson
    Publication
    Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cenp-B, INCENP-Δcen, GFP
    Tags
    GFP and full length CENP-B
    Expression
    Mammalian
    Mutation
    CEN box deleted from INCENP (aa1-46)
    Available since
    Sept. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    45238-plasmid-map-sequence-id-84496

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  11. lenti SYN-SVI-DIO-dCas9-VPR

    Plasmid
    #164576
    Purpose
    Expresses a SYN-driven, intron-containing dCas9-VPR fusion in which the part of the dCas9 cassette is double floxed and in inverted orientation (DIO). Requires Cre-dependent recombination.
    Depositor
    Jeremy Day
    Publication
    Carullo et al eNeuro. 2021 Jul 26. pii: ENEURO.0188-21
    Insert
    A DIO FLAG-dCas9-VPR cassette containing an SV40 intron
    Use
    CRISPR, Cre/Lox, and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Available since
    Feb. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    164576-plasmid-map-sequence-id-323657

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  13. Cenp-B INCENP GFP

    Plasmid
    #45238
    Purpose
    Target INCENP to centromeres via full length CENP-B
    Depositor
    Michael Lampson
    Publication
    Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cenp-B, INCENP-Δcen, GFP
    Tags
    GFP and full length CENP-B
    Expression
    Mammalian
    Mutation
    CEN box deleted from INCENP (aa1-46)
    Available since
    Sept. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    45238-plasmid-map-sequence-id-84496

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  18. ttAtCas12a+int

    Plasmid
    #182384
    Purpose
    Gives very high editing efficiency in barley. Also works in Brassica oleracea
    Depositor
    Wendy Harwood
    Publication
    Lawrenson et al bioRxiv 2022.04.28.489853
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LbCas12a coding sequence with D156R and Arabidopsis introns
    Use
    CRISPR
    Mutation
    D156R + 8 Arabidopsis introns added
    Promoter
    None
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182384-plasmid-map-sequence-id-361472

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Showing: 7401 - 7420 of 29164 results