We narrowed to 9,493 results for: pho;

1. LUC45 (L/Sec/mutP)

   Plasmid

   #112148

   Purpose

   Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

   Depositor

   Paul Copeland

   Article

   Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.

   Insert

   Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and MUTANT AUGA PHGPx SECIS core (Gpx4 Rat, Photinus pyralis)
   

   Expression

   Mammalian

   Mutation

   Cysteine 258 mutated to selenocysteine (U) and SE…

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTH728-CEN-RLuc/maxCFLuc

   Plasmid

   #38212

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Inserts

   Firefly Luciferase
   Renilla luciferase
   

   Expression

   Yeast

   Mutation

   All codons of the original FLuc gene were exchang…

   Promoter

   ADH1 and TDH3 (=GPD)

   Available Since

   Oct. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. CIP2Aprom1802bp-pGL4.10Luc

   Plasmid

   #60868

   Purpose

   Luc reporter driven by 1802 bp of the CIP2A promoter

   Depositor

   Jukka Westermarck

   Article

   Khanna et al PLoS One. 2011 Mar 22;6(3):e17979. doi:

   Insert

   1802 bp promoter fragment of Cancerous Inhibitor of PP2A (CIP2A Human)
   

   Use

   Luciferase; Promoterless

   Available Since

   April 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMRX-IP-hWIPI1A-3×FLAG

   Plasmid

   #215509

   Purpose

   Expresses FLAG tagged human WIPI1A.

   Depositor

   Noboru Mizushima

   Article

   Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

   Insert

   WD-repeat protein interacting with phosphoinositides 1A (WIPI1 Human)
   

   Use

   Retroviral

   Tags

   3×FLAG

   Expression

   Mammalian

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPICZ-mouseGIRK2-eGFP-StII-6xHis

   Plasmid

   #124277

   Purpose

   Expresses mouse GIRK2 as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tag

   Depositor

   Arthur Laganowsky

   Article

   Liu et al Nat Commun. 2019 Mar 22;10(1):1352. doi:

   Insert

   GIRK2 (Kcnj6 Mouse)
   

   Tags

   eGFP-StreptagII-6xHis

   Expression

   Yeast

   Promoter

   AOX1

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-tetO-Id2

   Plasmid

   #70764

   Purpose

   Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Id2

   Depositor

   Hubert Schorle

   Article

   Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

   Insert

   inhibitor of DNA binding 2 (Id2 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   tetO

   Available Since

   Nov. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3-mCherry-TSC2(AA,3Q)

   Plasmid

   #192444

   Purpose

   Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A, K1614Q, K1615Q, R1616Q mutations to disrupt Akt phosphorylation and GAP activity.

   Depositor

   Jin Zhang

   Article

   Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

   Insert

   mCherry-TSC2(S939A,T1439A,K1614Q,K1615Q,R1616Q ) (TSC2 Human)
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

   Promoter

   CMV

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5 FRT TO myc Separase Non-Cleavable

   Plasmid

   #59826

   Purpose

   Allows the integration of myc Separase Non-Cleavable in the genome and Tet-inducible expression.

   Depositor

   Stephen Taylor

   Article

   Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

   Insert

   Separase (ESPL1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Non-Cleavable (E1483R, R1486E, E1503R, R1506E, E1…

   Promoter

   hybrid human cytomegalovirus (CMV)/TetO2 promoter

   Available Since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSUMO CPTP

   Plasmid

   #170735

   Purpose

   The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

   Depositor

   Rhoderick Brown

   Article

   Simanshu et al Nature. 2013 Aug 22;500(7463):463-7. doi

   Insert

   CPTP (CPTP Human)
   

   Tags

   SUMO

   Expression

   Bacterial

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1_GP1Fc Cl13

    Plasmid

    #138599

    Purpose

    Expresses GP1 of LCMV Cl13 fused with human IgG (Fc fusion)

    Depositor

    Annette Oxenius

    Article

    Krautler et al Cell Rep. 2020 Jan 28;30(4):997-1012.e6.

    Insert

    LCMV GP1-Fc
    

    Tags

    Fc fusion protein

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBabe-TetCMV-puro-mVenus-PA-Rac1-T17N

    Plasmid

    #22018

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    mVenus-PA-Rac1-T17N (RAC1 Avena sativa (oat), Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Rac1 starts at I4 and contains mutation T17N.

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pTH726-CEN-RLuc/minCFLuc

    Plasmid

    #38210

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Expression

    Yeast

    Mutation

    All codons of the original FLuc gene were exchang…

    Promoter

    ADH1 and TDH3 (=GPD)

    Available Since

    Oct. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTIGER_tdTomato::3xFLAG::dCrk

    Plasmid

    #131138

    Purpose

    UASp construct for over-expression of Drosophila Crk with N-terminal tandem Tomato and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    Crk (Crk Fly)
    

    Tags

    3XFLAG and tdTomato

    Expression

    Insect

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA6-N-3XFLAG-Fzd6

    Plasmid

    #123588

    Depositor

    Bruce Beutler

    Article

    Choi et al Science. 2019 May 10;364(6440). pii: 364

    Insert

    frizzled class receptor 6 (Fzd6 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBabe-TetCMV-puro-mCerulean-PA-Rac1

    Plasmid

    #22031

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    mCerulean-PA-Rac1 (RAC1 Avena sativa (oat), Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Rac1 starts at I4 and contains mutations Q61L, E9…

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pMXs-puro HA-Atg14*dCC

    Plasmid

    #38266

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

    Insert

    autophagy related genes 14 (ATG14 Human)
    

    Use

    Retroviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    changed A618T, A621T, C624A and T627C for siRNA r…

    Promoter

    LTR

    Available Since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

17. GFP-CLCb (EED/QQN)

    Plasmid

    #47422

    Depositor

    Peter McPherson

    Article

    Poupon et al Proc Natl Acad Sci U S A. 2008 Jan 8;105

    Insert

    CLCb EED/QQN (CLTB Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    EED changed to QQN, in aa20-41 region

    Promoter

    CMV

    Available Since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

18. LENTICRISPR-HK2_sgRNA2

    Plasmid

    #201597

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    HK2 (HK2 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA HA-Gnat1 WT-3UTR

    Plasmid

    #184026

    Purpose

    Expresses mouse Gnat1 protein with N-terminal HA tag from cDNA that contains the full length 3'-UTR

    Depositor

    Peter Stoilov

    Article

    Matalkah et al Commun Biol. 2022 Sep 24;5(1):1011. doi:

    Insert

    Gnat1 (Gnat1 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBabe 3xFLAG human Bmf

    Plasmid

    #17242

    Depositor

    Joan Brugge

    Article

    Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

    Insert

    Bmf (BMF Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only