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169,450 results

Showing: 7421 - 7440 of 169450 results

  1. pGEX6P1-DEST-His

    Plasmid
    #119763
    Purpose
    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal 10xHis tag.
    Depositor
    Andrew Jackson, Martin Reijns
    Type
    Empty backbone
    Tags
    10xHis and GST
    Expression
    Bacterial
    Promoter
    tac promoter
    Available Since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    119763-plasmid-map-sequence-id-234026

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  4. mCherry-Calnexin-N-14

    Plasmid
    #55005
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Calnexin (CANX Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    I474N in Calnexin
    Promoter
    CMV
    Available Since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    55005-plasmid-map-sequence-id-456544

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  7. pEF1a-FB-dCas9-puro

    Plasmid
    #100547
    Purpose
    Expresses N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9 protein
    Depositor
    Jian Xu
    Article
    Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.
    Inserts
    N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9
    puromycin
    Use
    CRISPR
    Tags
    FLAG, Biotin-acceptor-site (Avitag)
    Expression
    Mammalian
    Promoter
    Human EF1a
    Available Since
    Sept. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    100547-plasmid-map-sequence-id-313417

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  9. pTU2-AmyE-Pveg-eGFP

    Plasmid
    #112792
    Purpose
    Expresses GFP under the control of Pveg promoter and it is integrated into AmyE locus. Used to test correct transformation
    Depositor
    Chris French
    Article
    Wicke et al bioRxiv 185108
    Insert
    Pveg-eGFP
    Expression
    Bacterial
    Available Since
    Aug. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    112792-plasmid-map-sequence-id-218394

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  11. pET29b-rhMOG

    Plasmid
    #225484
    Purpose
    Express the soluble domain of Myelin Oligodendrocyte Glycoprotein (rhMOG) in SHuffle E. coli
    Depositor
    John Pak
    Article
    Wu et al J Immunol Methods. 2024 Oct 13:113764. d
    Insert
    Soluble Domain of Myelin Oligodendrocyte Glycoprotein (MOG Human)
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-29 (signal peptide) and 150…
    Promoter
    T7
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    225484-plasmid-map-sequence-id-449491

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  12. pLenti6.2_mCherry_ mito_roGFP2-Orp1

    Plasmid
    #155042
    Purpose
    Fluorescent redox reporter
    Depositor
    Vanessa LaPointe
    Insert
    Orp1 (HYR1 Budding Yeast)
    Use
    Lentiviral
    Tags
    mitochondrial targeting sequence and roGFP2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    155042-plasmid-map-sequence-id-311567

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  17. pcDNA5/FRT/TO-GAlphai1-RLuc8

    Plasmid
    #140973
    Purpose
    Encodes a G alpha subunit (GNAl1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphai1-RLuc8 (GNAI1 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140973-plasmid-map-sequence-id-300655

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  19. qTAG-N-Puro-moxGFP-GOLGA2

    Plasmid
    #207792
    Purpose
    Donor template for Puro-2A-moxGFP insertion into the N-terminus of the GOLGA2 locus for Golgi visualization. To be co-transfected with sgRNA plasmid px330-PITCh-GOLGA2 Addgene #207791
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    GOLGA2 Homology Arms flanking a Puro-moxGFP Cassette (GOLGA2 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207792-plasmid-map-sequence-id-413795

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Showing: 7421 - 7440 of 169450 results