179,996 results

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pJZC74

Plasmid

#62342

Purpose

sgRNA + 1x COM with COM-KRAB effector for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Publication

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA + 1x COM binding module
COM-KRAB

Use

Lentiviral

Tags

KRAB

Expression

Mammalian

Mutation

Targets sv40 promoter, sequence: GAATAGCTCAGAGGCC…

Promoter

CMV and U6

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA4TO-mito-mCherry-24xGCN4_v1

Plasmid

#60913

Purpose

A fusion protein of the SunTag 24xGCN4 v1 peptide array to a mitochondrial targeting domain and mCherry

Depositor

Ron Vale

Publication

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mito-mCherry

Expression

Mammalian

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pX330A_D10A-1x2

Plasmid

#58772

Purpose

Expresses Cas9 nickase and gRNA

Depositor

Takashi Yamamoto

Publication

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pTara:500

Plasmid

#60717

Purpose

Expresses T7 RNAP from the arabinose inducible promoter PBAD with a RBS strength of 500 from the Salis RBS calculator. Also includes a constitutive araC ORF

Depositor

Matthew Bennett

Publication

Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Insert

T7 RNAP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PBAD

Available since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBMN-YFP-Parkin

Plasmid

#59416

Purpose

to make YFP-Parkin stable cell line with retrovirus

Depositor

Richard Youle

Publication

Yamano et al Elife (Cambridge). 2014 Feb 25;3:e01612.

Insert

YFP-Parkin (PRKN Human)

Use

Retroviral

Tags

EYFP

Expression

Mammalian

Available since

Aug. 22, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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FusionRed-pBAD

Plasmid

#54677

Purpose

Localization: Protein Expression Vector , Excitation: 580, Emission: 608

Depositor

Michael Davidson

Publication

Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

Type

Empty backbone

Tags

6xHis and FusionRed

Expression

Bacterial

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles

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pcDNA1 Flag ATXN1[85Q]

Plasmid

#33237

Depositor

Huda Zoghbi

Publication

Skinner et al Nature. 1997 Oct 30;389(6654):971-4.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Ataxin-1 (ATXN1 Human)

Tags

Flag

Expression

Mammalian

Mutation

insert contains a stretch of 85 Glutamines and no…

Promoter

CMV

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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mCardinal-Lifeact-7

Plasmid

#54663

Purpose

Localization: Actin, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Publication

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Lifeact-mCardinal

Expression

Mammalian

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles

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mVenus-N1

Plasmid

#54640

Purpose

Localization: N1 Cloning Vector, Excitation: 515, Emission: 528

Depositor

Michael Davidson, Atsushi Miyawaki

Publication

Nagai et al Nat Biotechnol. 2002 Jan;20(1):87-90.

Type

Empty backbone

Tags

mVenus

Expression

Mammalian

Available since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

8 Articles
CAG:: mCherry-2a-hM4Dnrxn

Plasmid

#52523

Purpose

Co-expresses hM4Dnrxn and a red fluorescent label

Depositor

Scott Sternson

Publication

Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mCherry-2a-hM4Dnrxn

Tags

2xHA

Expression

Mammalian

Promoter

CAg

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pEGFP-Kif2A

Plasmid

#52401

Purpose

Expresses GFP-tagged Kif2A in mammalian cells under the CMV promoter

Depositor

Gohta Goshima, Ryota Uehara

Publication

Uehara et al J Cell Biol. 2013 Aug 19;202(4):623-36.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Kif2A (KIF2A Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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3xFLAG-dCas9/pMXs-neo

Plasmid

#51260

Purpose

Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

Depositor

Hodaka Fujii

Publication

Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

Insert

3xFLAG-dCas9

Use

CRISPR and Retroviral

Tags

3xFLAG tag and NLS (nuclear localization signal)

Expression

Mammalian

Mutation

human codon-optimized, D10A + H840A

Promoter

LTR

Available since

Feb. 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMSCV-mCherry-Syk

Plasmid

#50045

Purpose

Expression of mCherry-Syk in mammalian cells

Depositor

Hidde Ploegh

Publication

Strijbis et al PLoS Pathog. 2013 Jun;9(6):e1003446. doi

Insert

Spleen tyrosine kinase (Syk) (Syk Mouse)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

pCMV

Available since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pJBEI-6410

Plasmid

#47049

Purpose

BglBrick plasmid (=pBbA5a-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coli

Depositor

Taek Soon Lee

Publication

Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

Insert

Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene

Use

Synthetic Biology; Bglbrick

Expression

Bacterial

Mutation

R364S mutation in MK

Promoter

PlacUV5

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCIneoEGFP

Plasmid

#46949

Purpose

GFP in Promega pCIneo (uses CMV promoter). Superior to pfwB for some purposes

Depositor

John Schiller

Publication

Buck et al Curr Protoc Cell Biol. 2007 Dec;Chapter

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GFP

Expression

Mammalian

Promoter

CMV

Available since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pFosCreER

Plasmid

#46388

Depositor

Liqun Luo

Publication

Guenthner et al Neuron. 2013 Jun 5;78(5):773-84. doi: 10

Inserts

Fos (Fos Mouse)
CreERT2

Use

Mouse Targeting

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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hPDI1_18-508_WT_pFLAG-CMV1

Plasmid

#31382

Depositor

David Ron

Publication

Zito et al Mol Cell. 2010 Dec 10. 40(5):787-97.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hPDI1 (P4HB Human)

Tags

FLAG M2

Expression

Mammalian

Available since

Jan. 23, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLKO.1-sh-hSOX9-1

Plasmid

#40644

Depositor

Bob Weinberg

Publication

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

sh-hSOX9-1 (SOX9 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hU6

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pFZ19

Plasmid

#36184

Purpose

Expression of genes under control of an estrogen-inducible 35S promoter

Depositor

Daniel Voytas

Publication

Zhang et al Proc Natl Acad Sci U S A. 2010 Jun 29. 1

Type

Empty backbone

Use

Plant t-dna vector

Expression

Plant

Promoter

XVE_35S (estrogen-inducible)

Available since

Aug. 24, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCMV-Tag2B EDD C2768A

Plasmid

#37189

Depositor

Darren Saunders, Charles Watts

Publication

Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-7

Insert

EDD C2768A (UBR5 Human)

Tags

6x His Tag and FLAG

Expression

Mammalian

Mutation

Changed Cysteine 2768 to Alanine

Promoter

CMV

Available since

July 26, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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