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We narrowed to 14,352 results for: Cas9

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Showing: 7461 - 7480 of 14352 results

  2. pESR1.1.0-gDNA

    Plasmid
    #112433
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ESR1
    Depositor
    Kevin White
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ESR1 (ESR1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Dec. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    112433-plasmid-map-sequence-id-230374

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  3. pOTTC763 - pX458 with rat Rosa26 gRNA A

    Plasmid
    #113161
    Purpose
    A plasmid that expresses a guide RNA targeting rat Rosa26 as well as FLAG-tagged SpCas9 and GFP
    Depositor
    Brandon Harvey
    Publication
    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNA for rat Rosa26
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    hU6
    Available since
    Feb. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    113161-plasmid-map-sequence-id-236005

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  4. B2M Bulldozer (gRNA crB2M_13)

    Plasmid
    #84381
    Purpose
    This plasmid expresses a gRNA targeting the first exon of human B2M. Most efficient gRNA targeting B2M, leading to ablation of B2M and MHC class I surface expression in 50% of transfected cells
    Depositor
    Chad Cowan
    Publication
    Mandal et al Cell Stem Cell. 2014 Nov 6;15(5):643-52.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    B2M Bulldozer crB2M_13 gRNA
    Expression
    Mammalian
    Promoter
    hU6 promoter
    Available since
    July 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    84381-plasmid-map-sequence-id-189203

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  5. pUC19-GCG-T2A-NLS-EmGFP-P2A-puro-PGK-puro

    Plasmid
    #121175
    Purpose
    Donor template for CRISPR-Cas9 mediated generation of GCG-NLS-EmGFP reporter cell lines
    Depositor
    Timo Otonkoski
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GCG-T2A-NLS-EmGFP-P2A-Puro-PGK-Puro
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121175-plasmid-map-sequence-id-235290

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  12. PB-PE SpRY_ATH51

    Plasmid
    #235998
    Purpose
    Expresses SpRY_ATH51 prime editor and pegRNA in mammalian cells
    Depositor
    Tobias Cantz
    Publication
    Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpRY_ATH51_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    bpSV40 NLS
    Expression
    Mammalian
    Mutation
    ectopic insertion of 7 amino acids of PID turn-he…
    Available since
    Aug. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    235998-plasmid-map-sequence-id-470119

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  16. pDONR-U6gRNA-PGKpuro2ABFP

    Plasmid
    #136405
    Purpose
    Donor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses Puromycin linked to TagBFP.
    Depositor
    Massimo Squatrito
    Publication
    Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Mutation
    BbsI restriction site at position 437 was removed…
    Promoter
    U6, PDK
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    136405-plasmid-map-sequence-id-269877

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  18. VRQR-HF1 variant

    Plasmid
    #138563
    Purpose
    Expresses human codon-optimized SpCas9-VRQR-HF1 and blasticidin resistance: EFS promoter-VRQR-HF1-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    VRQR-HF1
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138563-plasmid-map-sequence-id-274789

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  19. QQR1 variant

    Plasmid
    #138564
    Purpose
    Expresses human codon-optimized SpCas9-QQR1 and blasticidin resistance: EFS promoter-QQR1-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    QQR1
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    G1218R, N1286Q, I1331F, D1332K, R1333Q, R1335Q, T…
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138564-plasmid-map-sequence-id-274793

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  20. pMini-CMV-NLS-dead R-IscB_ADAR2dd-NES_T2A_mCherry (W98X)_U6-ωRNA

    Plasmid
    #246432
    Purpose
    All-in-one plasmid. Expresses R-IscB_ADAR2dd in mammalian cells for A-to-I RNA editing. Inactive mCherry included to assess A-to-I editing. Spacer included can direct mCherry fluorescence recovery.
    Depositor
    Ailong Ke
    Publication
    Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    O.gue IscB with dead mutations (D60A, H269A) and delta-TID, fused to human ADAR2 deaminase domain (ADARB1 Human, Synthetic, human gut metagenome)
    mCherry
    ωRNA
    Tags
    HIV NES, SGGSSGGSSGSETPGTSESATPESSGGSSGGS linker …
    Expression
    Mammalian
    Mutation
    R-IscB: changed Aspartic Acid 60 to Alanine, chan…
    Promoter
    CMV and U6
    Available since
    Nov. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    246432-plasmid-map-sequence-id-490908

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Showing: 7461 - 7480 of 14352 results