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pST39-His/Vps27(345-440) - Hse(275-375)

Plasmid

#17638

Depositor

James Hurley

Article

Prag et al Dev Cell. 2007 Jun . 12(6):973-86.

Insert

His/Vps27(345-440) – Hse1(275-375) (VPS27, HSE1 Budding Yeast)

Expression

Bacterial

Available Since

March 28, 2008

Availability

Academic Institutions and Nonprofits only
mCherry-miniSOG-Display-5

Plasmid

#54802

Purpose

Localization: Cytoplasm, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Type

Empty backbone

Tags

mCherry-miniSOG-Display

Expression

Mammalian

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only
H1N1-PR8_RBD

Plasmid

#251261

Purpose

To express the H1N1-PR8 influenza receptor binding domain in E. coli

Depositor

Yutaka Kuroda

Article

Tu et al Int J Mol Sci. 2024 Apr 1;25(7):3943. do

Insert

H1N1-PR8_RBD

Tags

6xHis, 11 aa linker, thrombin cleavage site

Expression

Bacterial

Available Since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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pPR-1_1A_5T_AATG

Plasmid

#250448

Purpose

GRASP module to assemble sPPR proteins for RNA editing: module 1A_5T_AATG

Depositor

Ian Small

Article

Dennis et al Nucleic Acids Res. 2025 Oct 28;53(20):gk

Insert

1A_5T_AATG

Use

Synthetic Biology

Available Since

April 10, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-BSD-T7-sgRNA

Plasmid

#245119

Purpose

Plasmid backbone to for inserting a T7 promoter driven guide RNA into the rDNA spacer of T. brucei

Depositor

Monica Mugnier

Article

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Type

Empty backbone

Use

CRISPR

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-PUR-T7-sgRNA

Plasmid

#245108

Purpose

Plasmid backbone to for inserting a T7 promoter driven guide RNA into the rDNA spacer of T. brucei

Depositor

Monica Mugnier

Article

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Type

Empty backbone

Use

CRISPR

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pTet_PflHamM(pre-cut)AB

Plasmid

#248942

Purpose

Hachiman from P. fluorescens under pTet control with HamM programmed breaks in the peptide backbone at I118 and I218

Depositor

Jennifer Doudna

Article

Tuck et al Science. 2026 Jan 8;391(6781):195-201. d

Insert

PflHamM(pre-cut)AB

Expression

Bacterial

Mutation

HamM programmed breaks in the peptide backbone at…

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pBS L30 ALFA-Apex-Rab7A

Plasmid

#224892

Purpose

Rab7A fused to APEX and an ALFA tag on N-term in a pBS L30 backbone.

Depositor

Raphael Gaudin

Article

Bare et al bioRxiv 2025.10.07.680694

Insert

Rab7A (RAB7A Human)

Tags

ALFA and APEX2

Expression

Mammalian

Promoter

Gibson Cloning

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti-sgStuffer-GFP-Puro

Plasmid

#208349

Purpose

Lentivirus sgRNA backbone with GFP and PuroR. Compatible with golden gate cloning (Esp3i/BsmBI)

Depositor

Peter S Nelson

Article

Frank et al eLife 2025

Type

Empty backbone

Use

CRISPR and Lentiviral

Promoter

hU6

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti-sgStuffer-mCherry-Puro

Plasmid

#208350

Purpose

Lentivirus sgRNA backbone with mCherry and PuroR. Compatible with golden gate cloning (Esp3i/BsmBI)

Depositor

Peter S Nelson

Article

Frank et al eLife 2025

Type

Empty backbone

Use

CRISPR and Lentiviral

Promoter

hU6

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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