166,485 results

1. pCIS GEM-CEPIA1er

   Plasmid

   #58217

   Purpose

   Ratiometric (Blue to Green) fluorescent indicator for calcium signaling in the endoplasmic reticulum

   Depositor

   Masamitsu Iino

   Article

   Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

   Insert

   GEM-CEPIA1er
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   GEM-GECO1 E298D F359W D400E E407D

   Promoter

   CAG

   Available Since

   July 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. p177bp-LacOx162-Hyg

   Plasmid

   #210215

   Purpose

   Integrates 162xLacO repeats with a hygromycin drug selection marker into 177bp repeat region.

   Depositor

   Calvin Tiengwe

   Article

   Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

   Insert

   162xLacO repeats derived from: PMID: 12864855.
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. LentiV_Neo_LKB1

   Plasmid

   #108111

   Purpose

   Lentiviral expression plasmid of human LKB1 cDNA with neomycin resistance gene

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

   Insert

   LKB1 (STK11 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EFS promoter

   Available Since

   April 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ166-OsPE2

   Plasmid

   #141080

   Purpose

   Gateway entry clone for CRISPR-Cas9(H840A) prime editing

   Depositor

   Yiping Qi

   Article

   Tang et al Mol Plant. 2020 Mar 25. pii: S1674-2052(

   Insert

   zCas9(H840A)-OsM-MLV-RT
   

   Use

   CRISPR; Gateway compatible zcas9(h840a)-osm-mlv-rt

   Tags

   3x FLAG, NLS and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   May 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-28a_6H-MMLV_RT_D524N-6H

   Plasmid

   #166945

   Purpose

   Bacterial expression of M-MLV reverse transcriptase

   Depositor

   Robert Tjian

   Article

   Graham et al PLoS One. 2021 Feb 3;16(2):e0246647. doi

   Insert

   gag-pol
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   D524N, H8Y

   Promoter

   T7

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR221 msNLRP3

   Plasmid

   #218639

   Purpose

   Gateway shuttling vector for cloning C-terminal NLRP3 tags (contains no stop codon).

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   NLRP3 (Nlrp3 Mouse)
   

   Use

   Gateway entry

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOpen-DpnIR

   Plasmid

   #165529

   Purpose

   Used to digest methylated DNA; to be expressed in dam- E. coli strains (strains which do not methylate their genomic DNA)

   Depositor

   Jennifer Molloy, Drew Endy, FreeGenes Project

   Insert

   DpnI
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 5, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pLminP_Luc2P_RE26

   Plasmid

   #90368

   Purpose

   C/EBPb - CEBPB gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CEBPb

   Available Since

   Aug. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGGS-FuG-B2

   Plasmid

   #67513

   Purpose

   This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. You can make type B2 envelope coating virus particle with HiRet by using this plasmid.

   Depositor

   Kazuto Kobayashi

   Article

   Kato et al J Neurosci. 2011 Nov 23;31(47):17169-79.

   Insert

   Fusion Glycoprotein type B2
   

   Use

   Lentiviral

   Tags

   Fusion protein of Vesicular stomatitis Indiana vi…

   Expression

   Mutation

   Promoter

   CAGGS

   Available Since

   July 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR221_SLC6A15

    Plasmid

    #131877

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC6A15 (SLC6A15 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 9, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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