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pLenti-CMV-Myr-Pink Flamindo

Plasmid

#204669

Purpose

Mammalian expression of membrane-bound Pink Flamindo for cAMP imaging

Depositor

Adam Cohen

Article

Xiang et al bioRxiv 2023

Insert

Myr-Pink Flamindo

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pAG1244 p65-mCherry-FIREtag

Plasmid

#205175

Purpose

Expresses p65-mCherry-FIREtag in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

p65-mCherry-FIREtag

Expression

Mammalian

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAG1242 NLS-mCherry-FIREtag

Plasmid

#205170

Purpose

Expresses NLS-mCherry-FIREtag in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

NLS-mCherry-FIREtag

Expression

Mammalian

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pSB-CMV-MCS-Puro GEM-LTB4

Plasmid

#202641

Purpose

Expresses the LTB4 sensor GEM-LTB4 in mammalian cells

Depositor

Balázs Enyedi

Article

Tamas et al Nat Commun. 2023 Aug 1;14(1):4610. doi:

Insert

GEM-LTB4

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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His6_MBP_FXR2P_P474S_P493N

Plasmid

#198695

Purpose

Expression of human FXR2 protein in E. coli

Depositor

Simpson Joseph

Article

Edwards et al Sci Rep. 2020 Sep 28;10(1):15858. doi: 1

Insert

FXR2P (FXR2 Human)

Tags

MBP

Expression

Bacterial

Mutation

Changed proline 474 to serine and proline 493 asp…

Promoter

T7

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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Tubulin-mhYFP

Plasmid

#186525

Purpose

Localization of monomeric hyperfolder YFP fluorescent protein to tubulin

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Monomeric hyperfolder YFP

Tags

Tubulin

Expression

Mammalian

Mutation

hfYFP-S147P/L195M/V206K

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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L1_UBE2:mS-Lt-Ck3

Plasmid

#136131

Purpose

L1 in position 3, mark cell outline with mScarletI

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpUBE2:mScarletI-Lti6b_3tNos-35S

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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L2_UBE2:eG-Lt-CsA

Plasmid

#136134

Purpose

Plasmid to transform Mp with constitutive promoter marking cell outline with eGFP. HygR

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-35S:HygR p5-MpUBE2:eGFP-Lti6b

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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MIGR1-CD5

Plasmid

#159329

Purpose

Over-expression of mouse CD5

Depositor

Nevil Singh

Article

Matson et al Proc Natl Acad Sci U S A. 2020 Jun 23;11

Insert

CD5 (Cd5 Mouse)

Use

Retroviral

Promoter

LTR

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-N-Flag-8His

Plasmid

#157677

Purpose

N-terminal tagging vector

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Flag-8His

Expression

Mammalian

Available Since

Aug. 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1-GFP-MTn-KDEL

Plasmid

#154348

Purpose

Mammalian expression of MTn

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

MTn

Tags

GFP

Expression

Mammalian

Available Since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-2PH-PLCd-mKate2

Plasmid

#109500

Purpose

Muscle specific zebrafish expression of mKate2 fused to 2x the PH domain from Human phospholipase C delta 1 (PLCD1, codon optimised). Binds PtdIns(4,5)P2.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

actc1b-2PH-PLCd-mKate2

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pOPS0379

Plasmid

#115510

Purpose

Suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing sfGFP

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Rlv3841pNifH(pOGG082) sfGFP (pOGG037) and T-pharma (pOGG003) assembled in pOGG026

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_GRB2-1/2

Plasmid

#91480

Purpose

Protein expression and purification of human SH3 domain construct GRB2-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

GRB2-1/2 (GRB2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;clecl14aCE1-P1Egfp

Plasmid

#90157

Purpose

contains clec14a enhancer sequence, and clec14a specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

clec14aE1P1Egfp

Tags

EGFP

Expression

Bacterial

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pRS424-TUB2

Plasmid

#60388

Purpose

Expression of yeast beta tubulin (TUB2) using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Expression

Yeast

Promoter

GAL

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
PA-mCherry-miniSOG-Lifeact-7

Plasmid

#54846

Purpose

Localization: Actin, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Lifeact-PAmCherry-miniSOG

Expression

Mammalian

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only
Keratin 18 S52A

Plasmid

#18060

Depositor

M Bishr Omary

Article

Ku et al J Cell Biol. 1994 Oct . 127(1):161-71.

Insert

Keratin 18 (KRT18 Human)

Expression

Mammalian

Mutation

S52A

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pBS Galectin1

Plasmid

#19216

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Galectin1 (LGALS1 Chicken)

Expression

Bacterial

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
7093 pNOTAG Hsp104 T317A

Plasmid

#1242

Depositor

Susan Lindquist

Article

Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

Insert

Hsp104 (T317A) (HSP104 Budding Yeast)

Expression

Bacterial

Mutation

T317A

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only