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CRISPRi non-coding library (CRiNCL) - Common to all 5 cancer cell lines

Pooled Library

#86539

Depositor

Jonathan Weissman

Article

Liu et al Science. 2017 Jan 06. Vol. 355, Issue 63

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only
pFW5000

Plasmid

#224214

Purpose

E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), p15A (E. coli replication origin), tetQ

Depositor

Ophelia Venturelli

Article

Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pJZ002

Plasmid

#120231

Purpose

CRISPR-cas9 targeting in E. coli with ampicillin resistance

Depositor

Bradley Moore

Article

Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

Type

Empty backbone

Use

CRISPR

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pNORg10

Plasmid

#249340

Purpose

Mediterraneibacter gnavus - Escherichia coli shuttle vector with catP selection marker

Depositor

Nozomu Obana

Article

Obana et al Nat Commun. 2026 Mar 12. doi: 10.1038/s4

Type

Empty backbone

Use

E. coli - r. gnavus shuttle vector

Available Since

March 25, 2026

Availability

Academic Institutions and Nonprofits only

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pFW2100

Plasmid

#224212

Purpose

Bacteroides genomic editing tool; IPTG inducible-Fncpf1

Depositor

Ophelia Venturelli

Article

Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

Insert

Fncpf1

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pFW6000

Plasmid

#224215

Purpose

Bacteroides genomic editing tool; aTc inducible-Fncpf1

Depositor

Ophelia Venturelli

Article

Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

Insert

Fncpf1

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pDM344

Plasmid

#108974

Purpose

shuttle vector

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum shuttle vector

Available Since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCV006-ermg

Plasmid

#232824

Purpose

Random transposon mutagenesis vector for Bacteroidales species - erythromycin

Depositor

Athanasios Typas

Insert

ermG

Expression

Bacterial

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pDM1020

Plasmid

#108977

Purpose

shuttle vector, C-terminal mCherry tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum shuttle vector

Tags

mCherry

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pDL1403

Plasmid

#226635

Purpose

This plasmid induces natural competence in classical biotype Vibrio cholerae and expresses the genes tfox and qstR.

Depositor

Andrew Camilli

Article

Woldetsadik et al mBio. 2024 Oct 8:e0245724. doi: 10.1128/

Inserts

tfox
qstR

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pJSK619

Plasmid

#205134

Purpose

Expression of SnxA-GFP in Dictyostelium

Depositor

Jason King

Article

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

SnxA

Use

Dictyostelium expression

Tags

EGFP

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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BASIC_5_Amp

Plasmid

#68139

Purpose

storage vector for BASIC part: Amp

Depositor

Geoffrey Baldwin

Article

Storch et al ACS Synth Biol. 2015 Apr 16.

Insert

Ampicillin

Expression

Bacterial

Available Since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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BASIC_1_MB1

Plasmid

#68135

Purpose

storage vector for BASIC part: MB1

Depositor

Geoffrey Baldwin

Article

Storch et al ACS Synth Biol. 2015 Apr 16.

Insert

MB1 origin

Expression

Bacterial

Available Since

Aug. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pJC162.1_pc2RNAi

Plasmid

#26541

Depositor

Phillip Newmark

Article

Collins et al PLoS Biol. 2010 . 8(10):e1000509.

Insert

prohormone convertase 2

Use

RNAi

Available Since

Oct. 28, 2010

Availability

Academic Institutions and Nonprofits only
pNO201

Plasmid

#249344

Purpose

Targeted gene disruption by use of a group II intron (targetron) vector in Clostridia

Depositor

Nozomu Obana

Article

Obana et al Nat Commun. 2026 Mar 12. doi: 10.1038/s4

Insert

group II intron and ItrA

Use

E. coli - clostridium shuttle vector

Available Since

March 25, 2026

Availability

Academic Institutions and Nonprofits only

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pDM1049

Plasmid

#108992

Purpose

Inducible expression vector (dox-on), C-terminal GFP tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum inducible expression vec…

Tags

GFP

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pDM1046

Plasmid

#108989

Purpose

Inducible expression vector (dox-on), N-terminal mCherry tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum inducible expression vec…

Tags

mCherry

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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GFPxm163

Plasmid

#193480

Purpose

Turn-off fluorescent sensor for chloride

Depositor

Sheel Dodani

Article

Peng et al Chem Sci. 2022 Oct 6;13(43):12659-12672.

Insert

GFPxm163

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pJC130.1-npp-18

Plasmid

#26505

Depositor

Phillip Newmark

Article

Collins et al PLoS Biol. 2010 . 8(10):e1000509.

Insert

npp-18

Use

RNAi

Available Since

Feb. 16, 2011

Availability

Academic Institutions and Nonprofits only
BASIC_2_Kan-MB1

Plasmid

#68136

Purpose

storage vector for BASIC part: Kan-MB1

Depositor

Geoffrey Baldwin

Article

Storch et al ACS Synth Biol. 2015 Apr 16.

Insert

Kan-MB1

Expression

Bacterial

Available Since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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