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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 7541 - 7560 of 9221 results

  1. MTK0_001

    Plasmid
    #123924
    Purpose
    Encodes the spCas9 gRNA GFP dropout expression cassette with ConLS and ConRE connectors with Ampicillin resistance as a type 0 part to be used in the MTK system
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    TU-sgRNA - L1/RE
    Expression
    Mammalian
    Available Since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    123924-plasmid-map-sequence-id-250078

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  2. MTK234_055

    Plasmid
    #123920
    Purpose
    Encodes the spCas9 gRNA GFP dropout expression cassette (bovine u6 promoter and constant region 2) as a type 234 part to be used in the MTK system
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    gRNA-GFPDROPOUT-for Sp Cas9 bu6_20N_cr2
    Expression
    Mammalian
    Available Since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    123920-plasmid-map-sequence-id-250070

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  3. pOCC215

    Plasmid
    #118879
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal Dendra2, and C-terminal HIS6 tag, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-Dendra2-3C-HIS6-stop-HindIII cassette
    Tags
    Dendra2, HIS6, cleavable with 3C protease
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118879-plasmid-map-sequence-id-232264

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  4. pOCC207

    Plasmid
    #118869
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal mCherry
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-mCherry-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease, mCherry
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118869-plasmid-map-sequence-id-232318

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  5. pLaNGo11-3

    Plasmid
    #126054
    Purpose
    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell
    Depositor
    Ralf-Bernd Klösgen
    Article
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Inserts
    chloroplastic FNR transit peptide
    ccdB cassette (BsaI)
    GFP11x3 (5 aa linker)
    Use
    Binary
    Tags
    GFP1-10
    Expression
    Plant
    Available Since
    Aug. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    126054-plasmid-map-sequence-id-254921

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  6. pMiNGo11-1

    Plasmid
    #126056
    Purpose
    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell
    Depositor
    Ralf-Bernd Klösgen
    Article
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Inserts
    mitochondria Rieske iron sulphur protein (1-100 aa)
    ccdB cassette (BsaI)
    GFP11
    Use
    Binary
    Tags
    GFP1-10
    Expression
    Plant
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    126056-plasmid-map-sequence-id-246974

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  9. pOCC230

    Plasmid
    #118871
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal mCitrine, cleavable with TEV
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C--NotI-ccdB-AscI-TEV-mCitrine-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease and mCitrine, cl…
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118871-plasmid-map-sequence-id-232332

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  10. pOCC69

    Plasmid
    #118874
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal monomeric GFP, cleavable with TEV
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C--NotI-ccdB-AscI-TEV-mGFP-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease and mGFP, cleava…
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118874-plasmid-map-sequence-id-232330

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  11. pOCC110

    Plasmid
    #118905
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6, cleavable with 3C, and C-terminal sortase site (LPETGg)
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-NotI-ccdB-AscI-LPETGg-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease and LPETGg
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118905-plasmid-map-sequence-id-232327

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  12. pOCC219

    Plasmid
    #118908
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal HIS10, cleavable with 3C protease and C-terminal YBBR tag
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-YBBR-3C-HIS10-stop-HindIII cassette
    Tags
    YBBR tag, HIS10, cleavable with 3C protease
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118908-plasmid-map-sequence-id-232256

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  15. pCALNL_Ch10

    Plasmid
    #81224
    Purpose
    pCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in PCDH15 locus on chromosome 10
    Depositor
    David Liu
    Article
    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw
    Insert
    gix-Neo-gix deletion cassette upstream of EGFP
    Expression
    Mammalian
    Promoter
    pCBa
    Available Since
    Nov. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    81224-plasmid-map-sequence-id-163294

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  18. pKRB156

    Plasmid
    #254233
    Purpose
    This pMV361-derived E. coli-Mycobacterium shuttle vector contains the iRFP670 and HoI driven by the hsp60 promoter, as well as a kanamycin resistance cassette.
    Depositor
    Manabu Ato
    Article
    Yamamoto et al Sci Rep. 2024 Oct 17;14(1):24343. doi: 1
    Inserts
    iRFP670
    HoI
    Expression
    Bacterial
    Promoter
    hsp60
    Available Since
    May 13, 2026
    Availability
    Academic Institutions and Nonprofits only
    254233-plasmid-map-sequence-id-507683

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Showing: 7541 - 7560 of 9221 results