We narrowed to 8,732 results for: Set

1. pBBK69 pHomL-RPL18Bp-SPC42-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

   Plasmid

   #179053

   Purpose

   Vector containing a yeast integration cassette for RPL18B-driven expression of SPC42-Link-Scarlet(rfp), a marker for the spindle pole

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   RPL18Bp-SPC42-Link-Scarlet(rfp)
   

   Expression

   Yeast

   Available Since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJW1820

   Plasmid

   #154321

   Purpose

   Multi-cassette for SapTrap

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   GFP^SEC (LoxP)^AID*::3xFLAG
   

   Use

   CRISPR and Cre/Lox

   Expression

   Worm

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBBK49 pHomL-RPL18Bp-Neon(gfp)-Link-TUB1-SSA1t-HomR (AmpR)

   Plasmid

   #179033

   Purpose

   Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-TUB1, a marker for the microtubules

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   RPL18Bp-Neon(gfp)-Link-TUB1
   

   Expression

   Yeast

   Available Since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOCC254

   Plasmid

   #118889

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal mCherry, cleavable with TEV

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-MBP-3C-mCherry-TEV-NotI-ccdB-AscI-stop-HindIII cassette
   

   Tags

   HIS6-MBP, cleavable with 3C protease; mCherry, cl…

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRS315-48nt-telo-I-SceI

   Plasmid

   #67623

   Purpose

   telomere cassette for telomere formation system containing 48 bp of S. pombe telomere repeats

   Depositor

   Kurt Runge

   Article

   Wang et al Mol Cell Biol. 2018 Jul 16;38(15). pii:

   Inserts

   3' gal1 unique sequence
   ura4 (ura4 Fission Yeast)
   48 bp telomere repeats
   hph
   

   Use

   Yeast cen/ars plasmid

   Available Since

   Nov. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMB1052:miniTol2-U6-(empty)SaCas9_gRNA-CAG-Cre-P2A-NeoR-WPRE

   Plasmid

   #168108

   Purpose

   Tol2 transposon vector of empty U6-S.aureus Cas9 gRNA cassette and CAG-driven Cre-NeoR

   Depositor

   Erich Jarvis

   Article

   Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

   Type

   Empty backbone

   Use

   CRISPR and Cre/Lox

   Available Since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRI012-pGEM-PacI-PU3-BsmbI-NotI

   Plasmid

   #140204

   Purpose

   Vector for cloning Cas9 sgRNA ( Step 1 of 2-step cloning). Contains AfU3 promoter driven sgRNA expression cassette flanked by PacI and NotI for further cloning in fungal vector.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology; Step 1 of cloning c…

   Promoter

   Aspergillus fumigatus U3 (RNAPIII).

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJW1759

   Plasmid

   #154315

   Purpose

   NT Donor-cassette for SapTrap

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   30aa linker::TEV::AID*::3xFLAG for NT slot
   

   Use

   CRISPR

   Expression

   Worm

   Available Since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJ02B2Gm_AE

   Plasmid

   #66063

   Purpose

   MoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Kanamycin plasmid [A:J23102:B:BCD2:C:E0040m:D:B0015:E]

   Depositor

   Douglas Densmore

   Article

   Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

   Insert

   FACS Controls
   

   Use

   Synthetic Biology

   Available Since

   Jan. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJ02B2Gm_EF

    Plasmid

    #66064

    Purpose

    MoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Kanamycin plasmid [E:J23102:B:BCD2:C:E0040m:D:B0015:F]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Insert

    FACS Controls
    

    Use

    Synthetic Biology

    Available Since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRII-Topo U6-SpAi9L-U6SpDMDL

    Plasmid

    #78608

    Purpose

    Expresses gRNAs (for SpCas9) targeting 5’ of Ai9 stop cassette and Dmd intron 22

    Depositor

    Amy Wagers

    Article

    Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

    Insert

    U6-SpAi9L-U6SpDMDL
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

12. pCRII-Topo U6-SpAi9R-U6-SpDMDR

    Plasmid

    #78609

    Purpose

    Expresses gRNAs (for SpCas9) targeting 3’ of Ai9 stop cassette and Dmd intron 23

    Depositor

    Amy Wagers

    Article

    Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

    Insert

    U6-SpAi9R-U6-SpDMDR
    

    Use

    Unspecified

    Promoter

    U6

    Available Since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

13. pUPD2 AtU6-26:LbCas12a_DR (GB1443)

    Plasmid

    #160575

    Purpose

    LbCas12a direct repeat sequence fused to the A. thaliana U6-26 promoter used for the assembly of gRNA expression cassettes.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

    Insert

    AtU6-26:LbCas12a_DR
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    U6-26

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA4/TO CD16.7-h-PKD1 FLM (4078-4303)

    Plasmid

    #83465

    Purpose

    Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and PKD1 sequence that corresponds to amino acids 4078 to 4303 of PC1 (98)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    Polycystin-1 (PKD1 Human)
    

    Tags

    CD16.7

    Expression

    Mammalian

    Mutation

    Amino-acids 4078-4303

    Available Since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. MTK0_046

    Plasmid

    #123976

    Purpose

    Encodes the Cas9 Thal5.10-2_hg18 homology destination with Hygromycin resistance cassette GFP dropout destination vector as a type 0 part to be used in the MTK systemh

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    hThal5.10-2_hg18 CAS9 Destination - HygroR
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJW1806

    Plasmid

    #154319

    Purpose

    NT Donor-cassette for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    linker::3xMyc for NT slot
    

    Use

    CRISPR

    Expression

    Worm

    Available Since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJW1805

    Plasmid

    #154318

    Purpose

    CT Donor-cassette for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    linker::3xMyc for CT slot
    

    Use

    CRISPR

    Expression

    Worm

    Available Since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJW1348

    Plasmid

    #154311

    Purpose

    NT Donor-cassette for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker for NT slot
    

    Use

    CRISPR

    Expression

    Worm

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJW1822

    Plasmid

    #154322

    Purpose

    Multi-cassette for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::GFP
    

    Use

    CRISPR

    Expression

    Worm

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pmTol-InsUAS-6MTnlstdTomato2A-Dusp6DM-CC

    Plasmid

    #67687

    Purpose

    UAS-driven 6xMyc nuclear localized tdTomato-2A-activated Dusp6 in minTol backbone; also with cry:cfp cassette

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3796-38

    Insert

    dusp6 (dusp6 Zebrafish)
    

    Tags

    6xmyc-NLS-tdTomato-2A

    Mutation

    S159A, S196A

    Available Since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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