We narrowed to 14,099 results for: cas9

1. NRK gRNA (BRDN0001147558)

   Plasmid

   #76314

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human NRK

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   NRK (NRK Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. TIE1 gRNA (BRDN0001147382)

   Plasmid

   #76686

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TIE1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TIE1 (TIE1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKMW493_U6-sgRNA-entry

   Plasmid

   #244823

   Purpose

   Mammalian expression of SpyCas9 single guide RNA with Golden Gate-compatible sgRNA spacer

   Depositor

   Jennifer Doudna

   Article

   Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

   Insert

   SpyCas9 single guide RNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDS48

   Plasmid

   #241839

   Purpose

   Cas9-gRNA construct targeting the UBE3A locus

   Depositor

   Albert Keung

   Article

   Kelkar et al Front Cell Dev Biol. 2025 Aug 29;13:1665

   Insert

   Cas9-gRNA (UBE3A )
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Available Since

   Sept. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-PE iSM

   Plasmid

   #235995

   Purpose

   Expresses iSM (iSpyMac) prime editor and pegRNA in mammalian cells

   Depositor

   Tobias Cantz

   Article

   Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

   Insert

   iSM_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
   

   Use

   CRISPR and Synthetic Biology; Piggybac transposon

   Tags

   bpSV40 NLS

   Expression

   Mammalian

   Available Since

   Aug. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pECNUS4_MP

   Plasmid

   #228358

   Purpose

   ABE8e-SpCas9-NG Multiplex Acceptor clone for insertion of up to six gRNA cassettes via Golden Gate with BpiI and T4 ligase, blue-white screening

   Depositor

   Eunyoung Chae

   Article

   Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Promoter

   RPS5A

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pICH86966::AtU6p::sgRNA:NbCysP6

   Plasmid

   #223217

   Purpose

   Expresses an sgRNA targeting the NbCysP6 gene in Nicotiana benthamiana with an Arabidopsis U6 promoter

   Depositor

   Tsepo Tsekoa

   Article

   Singh et al Front Plant Sci. 2022 Aug 18;13:953654.

   Insert

   NbCysP6 sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   None

   Expression

   Plant

   Promoter

   Arabidopsis U6 promoter

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-hSyn-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA

   Plasmid

   #177360

   Purpose

   AAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expression from Synapsin promoter

   Depositor

   Naoki Yamamoto

   Article

   Yamamoto et al Research Square [Preprint] 2021. doi: 10

   Inserts

   tdTomato
   Chimeric guide for SaCas9
   

   Use

   AAV

   Tags

   P2A and a tetracycline-controlled transactivator …

   Expression

   Mammalian

   Promoter

   Synapsine promoter and U6 promoter

   Available Since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-CMV-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA

   Plasmid

   #177365

   Purpose

   AAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expression

   Depositor

   Naoki Yamamoto

   Article

   Yamamoto et al Research Square [Preprint] 2021. doi: 10

   Inserts

   tdTomato
   Chimeric guide for SaCas9
   

   Use

   AAV

   Tags

   P2A and a tetracycline-controlled transactivator …

   Expression

   Mammalian

   Promoter

   CMV and U6 promoter

   Available Since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-CMV-scFVDnmt3L_bGHpA

    Plasmid

    #177350

    Purpose

    AAV expression of scFV-fused C terminal domain of Dnmt3l for targeted DNA methylation editing

    Depositor

    Naoki Yamamoto

    Article

    Yamamoto et al Research Square [Preprint] 2021. doi: 10

    Insert

    C-terminal domain of mouse Dnmt3l (Dnmt3l Mouse)
    

    Use

    AAV

    Tags

    myc and scFV

    Expression

    Mammalian

    Mutation

    194–415 aa

    Promoter

    CMV

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-CMV-scFVtet2_bGHpA

    Plasmid

    #177352

    Purpose

    AAV expression of scFV-fused catalytic domain of TET2 for targeted DNA methylation editing

    Depositor

    Naoki Yamamoto

    Article

    Yamamoto et al Research Square [Preprint] 2021. doi: 10

    Insert

    Tet Methylcytosine Dioxygenase 2 (TET2 Human)
    

    Use

    AAV

    Tags

    myc and scFV

    Expression

    Mammalian

    Mutation

    Catalytic domains of human TET2 (1129–1936 and 14…

    Promoter

    CMV promoter

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV-hSyn-scFVtet2_bGHpA

    Plasmid

    #177353

    Purpose

    AAV expression of scFV-fused catalytic domain of TET2 from Synapisin promoter for targeted DNA methylation editing

    Depositor

    Naoki Yamamoto

    Article

    Yamamoto et al Research Square [Preprint] 2021. doi: 10

    Insert

    Tet Methylcytosine Dioxygenase 2 (TET2 Human)
    

    Use

    AAV

    Tags

    myc and scFV

    Expression

    Mammalian

    Mutation

    Catalytic domains of human TET2 (1129–1936 and 14…

    Promoter

    human Synapsine promoter

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV-TRE-scFVtet2_bGHpA

    Plasmid

    #177358

    Purpose

    AAV expression of scFV-fused catalytic domain of TET2 from doxycycine-inducible promoter for targeted DNA methylation editing

    Depositor

    Naoki Yamamoto

    Article

    Yamamoto et al Research Square [Preprint] 2021. doi: 10

    Insert

    Tet Methylcytosine Dioxygenase 2 (TET2 Human)
    

    Use

    AAV

    Tags

    myc and scFV

    Expression

    Mammalian

    Mutation

    Catalytic domains of human TET2 (1129–1936 and 14…

    Promoter

    Tetracycline-dependent promoter

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pECNUS2

    Plasmid

    #184885

    Purpose

    ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery

    Depositor

    Eunyoung Chae

    Article

    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    RPS5A

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRetro-UMI

    Plasmid

    #222686

    Purpose

    CRISPR-screening

    Depositor

    Ulrich Elling

    Article

    Michlits et al Nat Methods. 2017 Dec;14(12):1191-1197.

    Type

    Empty backbone

    Use

    CRISPR and Retroviral

    Expression

    Mammalian

    Mutation

    PGK without BbsI cutsite

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. lentiGuide-puro_Panc480-MT7

    Plasmid

    #200941

    Purpose

    Multiplexed CRISPR array expressing 7 sgRNA in a lentiviral backbone

    Depositor

    James Eshleman

    Article

    Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

    Insert

    multiplexed sgRNA array
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6, EF-1a

    Available Since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pECNUS3

    Plasmid

    #184886

    Purpose

    ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery

    Depositor

    Eunyoung Chae

    Article

    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    RPS5A

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pECNUS1

    Plasmid

    #184884

    Purpose

    ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery

    Depositor

    Eunyoung Chae

    Article

    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    RPS5A

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDonor-PRF1-Exon3

    Plasmid

    #209075

    Purpose

    Targeting vector for the human PRF1 locus to replace exon 3 with repaired exon 3

    Depositor

    Klaus Rajewsky

    Article

    Li et al Sci Immunol. 2024 Feb 2;9(92):eadi0042.

    Insert

    PRF1-Exon3 HDRT (PRF1 Human)
    

    Use

    CRISPR

    Available Since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDonor-PRF1-T2A-cDNA

    Plasmid

    #209076

    Purpose

    Targeting vector for the human PRF1 locus to replace exon 3 with repaired cDNA

    Depositor

    Klaus Rajewsky

    Article

    Li et al Sci Immunol. 2024 Feb 2;9(92):eadi0042.

    Insert

    PRF1-T2A-cDNA HDRT (PRF1 Human)
    

    Use

    CRISPR

    Available Since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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