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pSUPER.retro.puro-shMePCE_shLARP7

Plasmid

#113538

Purpose

Retroviral vector designed to knock down MePCE and LARP7 simultaneously.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

shMePCE and shLARP7 (MEPCE Human)

Use

Retroviral

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.S72A

Plasmid

#106185

Purpose

Weak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Has Service

AAV1

Insert

SF-Venus-iGluSnFR.S72A

Use

AAV

Mutation

SFGFP: T203Y, F46L, T65G, S72A GltI: S72A

Promoter

hSynapsin

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 CMV NAPA-N BGH

Plasmid

#92278

Purpose

FRET probe; extracellular synapse targeted mCherry

Depositor

Baljit Khakh

Article

Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

Insert

Neuron-astrocyte proximity assay - Neuronal

Expression

Mammalian

Promoter

CMV

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAM5082

Plasmid

#85121

Purpose

PkaiB-kaiC-ecfp expressed from NSII-Gm vector constructed by seamless assembly. Fusion expressed as full length fusion protein at WT levels but is 5hr long period. Used only for co-localization exp

Depositor

Susan Golden

Article

Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d

Insert

PkaiB-KaiC-ECFP

Tags

ECFP

Expression

Bacterial

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pJK237

Plasmid

#73339

Purpose

Produces Acetobacter aceti 1023 citrate synthase with cleavable N-terminal His6/CBD/Ssp-intein fusion

Depositor

T. J. Kappock

Insert

citrate synthase

Tags

His6, Ssp intein, and chitin-binding domain

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pERB122

Plasmid

#58282

Purpose

const: miRFKBP5_GFP_IRES_Puro; ind: mC-FRB

Depositor

Michael Lampson

Article

Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

Inserts

FKBP-targeted miRNA
GFP
Puromycin resistance
inducible mCherry-FRB

Tags

mCherry

Expression

Mammalian

Available Since

April 6, 2015

Availability

Academic Institutions and Nonprofits only

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mEos2-LaminB1-10

Plasmid

#57395

Purpose

Localization: Nuclear Envelope, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

LaminB1 (LMNB1 Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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FLM-PC1Δ30

Plasmid

#41568

Depositor

Thomas Weimbs

Article

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Tags

CD16.7

Expression

Mammalian

Mutation

Δ30; sequence of PKD1 from 4078 - 4273

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
FLM-PC1Δ45

Plasmid

#41569

Depositor

Thomas Weimbs

Article

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Tags

CD16.7

Expression

Mammalian

Mutation

Δ45; sequence of PKD1 from 4078 - 4258

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
FLM-PC1Δ103

Plasmid

#41571

Depositor

Thomas Weimbs

Article

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Tags

CD16.7

Expression

Mammalian

Mutation

Δ103; sequence of PKD1 from 4078 - 4200

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
pXeX-SHH

Plasmid

#31233

Depositor

Cliff Ragsdale

Article

Agarwala et al Science. 2001 Mar 16. 291(5511):2147-50.

Insert

SHH (SHH Chicken)

Use

Xenopus expression vector

Mutation

starts at aa4

Available Since

Aug. 23, 2012

Availability

Academic Institutions and Nonprofits only
pENTR macroH2A1.2-C

Plasmid

#30512

Depositor

John Gurdon

Article

Pasque et al EMBO J. 2011 May 6;30(12):2373-87. doi:

Insert

macroH2A1.2-C (Macroh2a1 Mouse)

Use

Gateway - donor vector

Mutation

NO STOP

Available Since

June 25, 2012

Availability

Academic Institutions and Nonprofits only

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pMIY-mETV3

Plasmid

#34578

Depositor

Peter Murray

Article

El Kasmi et al J Immunol. 2007 Dec 1;179(11):7215-9.

Insert

Etv3 (Etv3 Mouse)

Use

Retroviral

Tags

IRES-YFP

Expression

Mammalian

Available Since

Dec. 16, 2011

Availability

Academic Institutions and Nonprofits only
pDONR223-NEK3

Plasmid

#23821

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NEK3 (NEK3 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-NEK10

Plasmid

#23437

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NEK10 (NEK10 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
PL1

Plasmid

#12394

Depositor

David Baker

Article

Dantas et al J Mol Biol. 2003 Sep 12. 332(2):449-60.

Insert

PL1

Tags

6xHis

Expression

Bacterial

Mutation

RosettaDesign was used to design sequences. Align…

Available Since

March 13, 2007

Availability

Academic Institutions and Nonprofits only
PL2

Plasmid

#12395

Depositor

David Baker

Article

Dantas et al J Mol Biol. 2003 Sep 12. 332(2):449-60.

Insert

PL2

Tags

6xHis

Expression

Bacterial

Mutation

RosettaDesign was used to design sequences. Align…

Available Since

March 13, 2007

Availability

Academic Institutions and Nonprofits only

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RCAS (A) Caronte (CT#382)

Plasmid

#13862

Depositor

Cliff Tabin

Article

Yokouchi et al Cell. 1999 Sep 3. 98(5):573-83.

Insert

Caronte (CER1 Chicken)

Use

Retroviral; Avian expression

Available Since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only
7851 pUG72-HSC82in

Plasmid

#10894

Depositor

Susan Lindquist

Article

Cowen et al Science. 2005 Sep 30. 309(5744):2185-9.

Insert

HSC82 (HSC82 Budding Yeast)

Use

Cre/Lox

Expression

Yeast

Available Since

April 26, 2006

Availability

Academic Institutions and Nonprofits only

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pSL141

Plasmid

#252930

Purpose

Label Lactococcus lactis with mCherry

Depositor

Caroline Ajo-Franklin

Article

Li et al Nat Biotechnol (2026).

Insert

mCherry

Expression

Bacterial

Promoter

P23

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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