We narrowed to 3,449 results for: CAMP

1. pcDNA3-AKAP79-AKAR4

   Plasmid

   #181957

   Purpose

   Genetically encoded FRET-based PKA activity reporter fused to AKAP79.

   Depositor

   Jin Zhang

   Article

   Tenner et al Elife. 2020 Nov 17;9. pii: 55013. doi: 1

   Insert

   AKAP79-AKAR4 (AKAP5 Human)
   

   Tags

   AKAP79, Cerulean, HIS tag, and cpVenus(E172)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)-FluoSTEP-AKAR

   Plasmid

   #181844

   Purpose

   Genetically encoded FRET-based sensor for monitoring PKA activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   FluoSTEP-AKAR
   

   Tags

   mRuby2 and sfGFP(1-10)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+)-PKAcat-mCherry

   Plasmid

   #181841

   Purpose

   mCherry-tagged PKA catalytic subunit for mammalian expression.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   PKAcat-mCherry (PRKACA Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV Syn 2xLyn-ERex-Venus-bPAC(F198Y)

   Plasmid

   #165491

   Purpose

   Neuronal expression of PACmn, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity .

   Depositor

   Thomas Oertner

   Article

   Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

   Insert

   2xLyn-ERex-Venus-bPAC(F198Y)
   

   Use

   AAV

   Tags

   Venus

   Expression

   Mammalian

   Promoter

   hSyn1

   Available Since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEM-HE-Glyco-Venus-bPAC(S27A)

   Plasmid

   #165490

   Purpose

   Xenopus oocyte expression of a photoactivatable adenylyl cyclase derived from bPAC using glycophorin for anchoring to the membrane with reduced dark activity

   Depositor

   Thomas Oertner

   Article

   Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

   Insert

   Glyco-Venus-bPAC(S27A)-Myc
   

   Use

   Cdna expression, xenopus oocyte

   Tags

   Myc and Venus

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-P2A-CIAR_pLenti_CMV_Puro

   Plasmid

   #86503

   Purpose

   Lenti plasmid for generating CIAR expressing stable cell lines

   Depositor

   Dustin Maly

   Article

   Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

   Insert

   GFP-P2A-CIAR (SOS1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   T968L in SOScat

   Promoter

   CMV

   Available Since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. NC3 pCDNA3.1 FLAG WTX (1-804)

   Plasmid

   #36958

   Depositor

   Randall Moon

   Article

   Major et al Science. 2007 May 18;316(5827):1043-6.

   Insert

   WTX (AMER1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 24, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3-EGFP-AC8(1-106)

   Plasmid

   #181958

   Purpose

   Residues 1-106 of rat adenylyl cyclase 8 (AC8) tagged with EGFP.

   Depositor

   Jin Zhang

   Article

   Tenner et al Elife. 2020 Nov 17;9. pii: 55013. doi: 1

   Insert

   EGFP-AC8(1-106) (Adcy8 Rat)
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. CRAF_S259A_pcDNA3.1

   Plasmid

   #86505

   Purpose

   Expresses CRAF_S259A in mammalian cells

   Depositor

   Dustin Maly

   Article

   Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

   Insert

   CRAF (RAF1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S259A

   Promoter

   CMV

   Available Since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEM-HE-2xLyn-ERex-Venus-bPAC(F198Y)

    Plasmid

    #165489

    Purpose

    Xenopus oocyte expression of PACmn, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity

    Depositor

    Thomas Oertner

    Article

    Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

    Insert

    2xLyn-ERex-Venus-bPAC(F198Y)
    

    Use

    Cdna expression, xenopus oocyte

    Tags

    Venus

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV gfaABC1D 2xLyn-ERex-Venus-bPAC(F198Y)

    Plasmid

    #171117

    Purpose

    Astrocyte expression of PACmn, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity

    Depositor

    Thomas Oertner

    Article

    Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

    Insert

    2xLyn-ERex-Venus-bPAC(F198Y)
    

    Use

    AAV

    Tags

    Venus

    Expression

    Mammalian

    Promoter

    gfaABC1D

    Available Since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CaMKII-2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)

    Plasmid

    #165493

    Purpose

    Neuronal expression of PACmn with dark Venus, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity

    Depositor

    Thomas Oertner

    Article

    Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

    Insert

    2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)
    

    Use

    AAV

    Tags

    darkVenus

    Expression

    Mammalian

    Promoter

    CaMKII

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV hSyn1 CaRhAC T2A tDimer

    Plasmid

    #101722

    Purpose

    - humanized - variant of pAAV hSyn YFP-CaRhAC Addgene # 101721– less reliable in hippocampal neurons

    Depositor

    Thomas Oertner

    Article

    Scheib et al Nat Commun. 2018 May 24;9(1):2046. doi:

    Inserts

    CatRhAC
    red fluorescent protein
    

    Use

    AAV

    Mutation

    E497K,C569D

    Promoter

    human Synapsin1 promotor and ribosomal skip seque…

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. CIAR_pcDNA5/FRT/TO

    Plasmid

    #86502

    Purpose

    Expresses CIAR in mammalian cells. Used to generate Flp-In stables.

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

    Insert

    CIAR (SOS1 Human)
    

    Use

    Frt

    Expression

    Mammalian

    Mutation

    T968L in SOScat

    Promoter

    CMV (tet operator)

    Available Since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-Syn-2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)

    Plasmid

    #165492

    Purpose

    Neuronal expression of PACmn with dark Venus, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity

    Depositor

    Thomas Oertner

    Article

    Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

    Insert

    2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)
    

    Use

    AAV

    Tags

    darkVenus

    Expression

    Mammalian

    Promoter

    hSyn1

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. GFP-RanV19 E1RA2

    Plasmid

    #118317

    Purpose

    Expression of human Ran

    Depositor

    Philip Stork

    Article

    Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

    Inserts

    RanV19 (RAN Human)
    EPac1RA2 (RAPGEF3, RAPGEF4 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    in-frame insertion of RanV19 into GFPEpac1RA2 and…

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. NC1 pGLUE WTX (1-804)

    Plasmid

    #36956

    Depositor

    Randall Moon

    Article

    Major et al Science. 2007 May 18;316(5827):1043-6.

    Insert

    WTX (AMER1 Human)
    

    Tags

    CBP tag, HA, and streptag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 24, 2012

    Availability

    Academic Institutions and Nonprofits only

18. xCIAR_pcDNA5/FRT/TO

    Plasmid

    #86504

    Purpose

    Expresses inactive control CIAR (xCIAR) in mammalian cells. Used to generate Flp-In stables.

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

    Insert

    xCIAR (SOS1 Human)
    

    Use

    Frt

    Expression

    Mammalian

    Mutation

    F929A and T968L in SOScat

    Promoter

    CMV (tet operator)

    Available Since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRSV-PKImut-v2

    Plasmid

    #45067

    Purpose

    Expression of intactive PKI (PKA inhibitor) mutant

    Depositor

    Richard Maurer

    Insert

    cAMP-dependent protein kinase inhibitor alpha
    

    Expression

    Mammalian

    Mutation

    changed Arg 20 & 21 to Glycines

    Promoter

    RSV

    Available Since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-RIαΔ(D/D+linker)-EGFP

    Plasmid

    #181843

    Purpose

    GFP-tagged PKA RIα subunit lacking the docking/dimerization (D/D) and linker regions; for mammalian expression.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    RIα-(D/D+linker)EGFP (PRKAR1A Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deletion of residues 12-112 in PKA RIα.

    Promoter

    CMV

    Available Since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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