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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 3,869 results for: SPL;

Showing: 741 - 760 of 3869 results

  1. pcDNA3-FRB-VMAc-CGFP

    Plasmid
    #70226
    Purpose
    Expresses C-terminal portion of GFP along with VMA intein for protein splicing
    Depositor
    Perry Howard
    Article
    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:
    Insert
    eGFP
    Use
    Tags
    FKBP-rapamycin binding (FRB) domain of the mammal…
    Expression
    Mammalian
    Mutation
    Changed Ile 129 to Cys, Deleted amino acids 1-128
    Promoter
    CMV
    Available since
    Nov. 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  2. pFP.R275

    Plasmid
    #138224
    Purpose
    Expresses the split mCherry reporter interrupted by the PhoRadA intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(SGK).PhoRadA.(TQL)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    P(araBAD) and iPC
    Available since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138224-plasmid-map-sequence-id-273527

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  6. pFP.R371

    Plasmid
    #138241
    Purpose
    Expresses the split mCherry reporter interrupted by the MP-M-DnaB artificially mini-intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(NGK).MP-M-DnaB*.(TEL)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    P(araBAD) and iPC
    Available since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138241-plasmid-map-sequence-id-273486

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  7. nGR2175-gs10-NLS-VPR

    Plasmid
    #188264
    Purpose
    Plasmid ensures constitutive expression of the N-terminal fragment of split GR2 (aa 1-175), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus
    Depositor
    Roman Jerala
    Article
    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038
    Insert
    nGR2175
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    188264-plasmid-map-sequence-id-374534

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  10. NSarQ27G-VMAn-FKBP

    Plasmid
    #70230
    Purpose
    Expresses N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicing
    Depositor
    Perry Howard
    Article
    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:
    Insert
    alpha Sarcin (N-terminal portion)
    Use
    Tags
    FK506- and rapamycin-binding protein (FKBP), Link…
    Expression
    Mammalian
    Mutation
    Changed Gln 27 to Gly
    Promoter
    CMV
    Available since
    Nov. 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  11. pFP.R400

    Plasmid
    #138175
    Purpose
    Expresses the split mCherry reporter interrupted by the chimeric Npu/SspDnaE intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(GGK).NpuSsp*.(CWN)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    P(araBAD) and iPC
    Available since
    May 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    138175-plasmid-map-sequence-id-296599

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  12. pUM31dao

    Plasmid
    #87242
    Purpose
    Plastid expression cassette containing Schizosaccharomyces pombe D-amino acid oxidase gene. Provides dual positive (D-alanine) and negative (D-valine) selection in transplastomic plants
    Depositor
    Anil Day
    Article
    Gisby et al Plant Physiol. 2012 Dec;160(4):2219-26.
    Insert
    D-amino oxidase
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    plastid rrn
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    87242-plasmid-map-sequence-id-211979

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  17. NODALvar-CAG-AAVS1

    Plasmid
    #115262
    Purpose
    For stable integration and expression (under control of the CAG promoter) of a human NODAL splice variant from the AAVS1 safe harbour locus in human cells
    Depositor
    Lynne Postovit
    Article
    Findlay et al (2018) bioRxiv 276170
    Insert
    NODAL (NODAL Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Sept. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    115262-plasmid-map-sequence-id-222505

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  18. pFP.R375

    Plasmid
    #138245
    Purpose
    Expresses the split mCherry reporter interrupted by the NrdA-2 intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(SNL).NrdA-2*.(CNE)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    P(araBAD) and iPC
    Available since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138245-plasmid-map-sequence-id-273498

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Showing: 741 - 760 of 3869 results