We narrowed to 7,742 results for: ef1a;

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pAAV-EF1a-mitoGFP-DIO

Plasmid

#174112

Purpose

Cre-dependent expression of mitochondria-targeted Green Fluorescent Protein under control of the EF1a promoter

Depositor

Maarten Kole

Article

Kole et al Nat Commun. 2022 Dec 9;13(1):7598. doi:

Has Service

AAV2

Insert

mitoGFP (COX8A Human)

Use

AAV

Tags

Cox8 targeting sequence and GFP

Promoter

Ef1a

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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qTAG-AAVS1-Ef1a-Zeo

Plasmid

#227268

Purpose

Empty AAVS1 targeting donor for the insertion of Zeo and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO_EGFP-GFE3

Plasmid

#79871

Purpose

For generating adeno-associated virus to express GFE3 under the EF1a promoter in a 'Cre-on' dependent manner.

Depositor

Don Arnold

Article

Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

Has Service

AAV1

Insert

GFP-GFE3

Use

AAV, Cre/Lox, and Synthetic Biology

Tags

GFP and HA

Expression

Mammalian

Promoter

EF1a

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-Ef1a-InteinC-CreC

Plasmid

#187615

Purpose

AAV vector encoding C-terminal portion of split-Intein-mediated split-Cre

Depositor

Il Hwan Kim

Article

Kim et al Cell Rep. 2022 Jun 7;39(10):110906. doi:

Insert

InteinC-CreC

Use

AAV

Expression

Mammalian

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pHR Ef1a_mAbCII-synNotch_tTA-PGK_Puro

Plasmid

#223538

Purpose

Anti-collagen type II scFv-based synNotch; puro-selectable

Depositor

Jonathan Brunger

Article

Walton et al Osteoarthritis Cartilage. 2025 Feb;33(2)

Insert

mAbCII_synNotch-tTA

Use

Lentiviral

Tags

c-myc tag

Expression

Mammalian

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pOTTC414 - pAAV EF1a hPREP

Plasmid

#59967

Purpose

An AAV packaging vector that expresses hPREP under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

Insert

Prolyl oligopeptidase (PREP Human)

Use

AAV

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 8, 2014

Availability

Academic Institutions and Nonprofits only

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EF1a-TALEN_HD (SPC right)

Plasmid

#126695

Purpose

right TALENS for introducing a double strand break into the genomic human SFTPC locus at the ATG start site

Depositor

Darrell Kotton

Article

Jacob et al Cell Stem Cell. 2017 Oct 5;21(4):472-488

Insert

human SPC right

Use

TALEN

Tags

NLS, truncated N term and TAL, FokI

Expression

Mammalian

Mutation

R451P in SPC

Promoter

EF1

Available Since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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EF1a-TALEN_NN (SPC left)

Plasmid

#126694

Purpose

left TALENS for introducing a double strand break into the genomic human SFTPC locus at the ATG start site

Depositor

Darrell Kotton

Article

Jacob et al Cell Stem Cell. 2017 Oct 5;21(4):472-488

Insert

human SPC left

Use

TALEN

Tags

0.5 repeat NN, TAL, FokI and NLS, truncated N ter…

Expression

Mammalian

Promoter

EF1

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Ef1a-CreN-InteinN

Plasmid

#187614

Purpose

AAV vector that encodes N-terminal portion of split-Intein-mediated split-Cre

Depositor

Il Hwan Kim

Article

Kim et al Cell Rep. 2022 Jun 7;39(10):110906. doi:

Insert

CreN-InteinN

Use

AAV

Expression

Mammalian

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-hMLH1dn-Hygro

Plasmid

#195512

Purpose

Lentiviral expression plasmid of hMLH1dn with P2A-HygroR marker

Depositor

Hyongbum Kim

Article

Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

Insert

hMLH1dn-P2A-HygroR (MLH1 Human)

Use

Lentiviral

Tags

P2A

Expression

Mammalian

Mutation

Human MLH1 del754-756

Promoter

EF-1a

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-ArgiNLS-EGFP

Plasmid

#220594

Purpose

Cre-dependent expression of a single-cell discriminating version of EGFP fluorescent protein.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

EGFP

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a-3NLS-NGN2TALE-GCN4

Plasmid

#120547

Purpose

Express 3xNLS-NGN2 TALE-GCN4 engineered to bind a site in the human NGN2 ene

Depositor

Yu Wang

Article

Zhao et al ACS Chem Biol. 2018 Mar 16;13(3):609-617

Insert

NLS, NGN2 TALE, GCN4 (NEUROG2 Synthetic)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1α

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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p5E-EF1a (JDW 1164)

Plasmid

#224473

Purpose

Gateway compatible 5' entry clone containing the human EF1a promoter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EF1a promoter

Expression

Mammalian

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_KRAS(G12V)-P2A-Hygro_Barcode

Plasmid

#170237

Purpose

Barcoded lentiviral vector to express KRAS (G12V) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

KRAS (KRAS Human)

Use

Lentiviral

Mutation

G12V

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pHLS-EF1a-FRB-SpCas9-A

Plasmid

#138477

Purpose

Expresses FRB fused SpCas9 for NanoMEDIC packaging.

Depositor

Akitsu Hotta

Article

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

SpCas9, human codon optimized

Use

CRISPR

Tags

3x HA tag and FRB

Expression

Mammalian

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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Ef1a-DIO-Aion-citrine

Plasmid

#192571

Purpose

AAV-vector for optogenetic long-term silencing of Cre-positive neurons

Depositor

Simon Wiegert

Insert

Aion

Use

AAV

Tags

Citrine

Expression

Mammalian

Mutation

T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

Promoter

Ef1-alpha

Available Since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

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Ef1a-DIO-somAion-citrine

Plasmid

#192573

Purpose

AAV-vector for optogenetic long-term silencing of Cre-positive neurons

Depositor

Simon Wiegert

Insert

somAion

Use

AAV

Tags

Citrine - soma-targeting motif from Kv2.1 channel

Expression

Mammalian

Mutation

T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

Promoter

Ef1-alpha

Available Since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

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p2514-pHAGE2-EF1aL-tdTomato-W

Plasmid

#248971

Purpose

Lentiviral expression of tdTomato

Depositor

Darrell Kotton

Insert

tdTomato

Use

Lentiviral

Promoter

EF1aL

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAID-EF1a linearizing in pX330

Plasmid

#140610

Purpose

Crispr/Cas9 plasmid for linearization of pAID-EF1a

Depositor

Tatsuo Fukagawa

Article

Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

Insert

sgRNA for pAID-EF1a plasmid digestion

Use

CRISPR

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pAF0474 Ef1a-Si74_attB acceptor

Plasmid

#193475

Purpose

Acceptor plasmid for 3 plasmid recombination assay

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Si74_attB

Expression

Mammalian

Promoter

Ef1a

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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