We narrowed to 7,458 results for: ef1a;

1. pAAV-Ef1a-DO-EGFP-WPRE-pA

   Plasmid

   #37085

   Purpose

   Expresses EGFP in cells lacking Cre (Cre-Off)

   Depositor

   Bernardo Sabatini

   Article

   Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

   Insert

   EGFP
   

   Use

   AAV and Cre/Lox; Cre-off

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   July 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHL-H1-ccdB-mEF1a-RiH

   Plasmid

   #60601

   Purpose

   Cloning vector for CRISPR-sgRNA (into the BamHI-EcoRI site), expresses RFP and hygromycin resistance gene.

   Depositor

   Akitsu Hotta

   Article

   Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

   Inserts

   Red Fluorescent Protein
   Hygromycin resistance gene
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. vYM112_PB-pEF1a-optiPIN2-HA(Hygro)

   Plasmid

   #160045

   Purpose

   For expressing the active auxin pump PIN2

   Depositor

   Michael Elowitz

   Article

   Ma et al Cell. 2022 Mar 17;185(6):967-979.e12. do

   Insert

   PIN2
   

   Use

   Synthetic Biology

   Tags

   HA

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   pEF1s

   Available Since

   April 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-U6-DR-SgRNA-DR-EF1a-mCherry-SV40

   Plasmid

   #154002

   Purpose

   Empty SgRNA expression system matching CasRx

   Depositor

   Hui Yang

   Article

   He et al Protein Cell. 2020 Mar 18. pii: 10.1007/

   Insert

   SgRNA expression system
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6, EF1a

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-Hygro-EF1A-LivePARBackbone

   Plasmid

   #176526

   Purpose

   Expression vector with a BamHI site in-frame with a Gly-Ser linker fused to EGFP; serves as the backbone for PAR binding domain incorporation for LivePAR

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSMPUW_6.14_M4_Ef1a-HA-gateway-SV40-Zeo

   Plasmid

   #128450

   Purpose

   lentiviral destination vector with Ef1a promoter, N-terminal HA tag, SV40 promoter, and Zeo Resistance gene

   Depositor

   Lindy Barrett

   Insert

   Ef1a promoter, N-terminal HA tag, SV40 promoter, and Zeocin Resistance gene
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRRLNeo-pEF1a-p53ash-mCerulean

   Plasmid

   #69579

   Purpose

   Expresses p53 tagged with mCerulean

   Depositor

   Galit Lahav

   Article

   Gaglia et al Proc Natl Acad Sci U S A. 2013 Sep 17;11

   Insert

   p53 (TP53 Human)
   

   Use

   Lentiviral

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   7 silent mutations in p53 DNA to prevent targetin…

   Promoter

   EF1alpha

   Available Since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-Ef1a-DO-mCherry-WPRE-pA

   Plasmid

   #37119

   Purpose

   Expresses mCherry in cells lacking Cre (Cre-Off)

   Depositor

   Bernardo Sabatini

   Article

   Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

   Insert

   mCherry
   

   Use

   AAV and Cre/Lox; Cre-off

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   July 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. Ef1a-DIO-BiPOLES-mCerulean

   Plasmid

   #154949

   Purpose

   Optogenetic tool for blue-light inhibition and red-light excitation of neurons

   Depositor

   Simon Wiegert

   Article

   Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

   Insert

   BiPOLES
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1-alpha

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-EF1alpha-soCoChR-tdTomato

    Plasmid

    #107714

    Purpose

    A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. EF1alpha promoter

    Depositor

    Edward Boyden

    Article

    Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

    Insert

    soCoChR-tdTomato
    

    Use

    AAV

    Tags

    KA2 and tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMR506-EF1a-H2B-EGFP

    Plasmid

    #186627

    Purpose

    Expresses nuclear-localised EGFP under EF1a promoter. For insertion of genetic barcodes into 3'UTR of EGFP.

    Depositor

    Jonas Frisén

    Article

    Ratz et al Nat Neurosci. 2022 Mar;25(3):285-294. do

    Insert

    EF1a (EEF1A1 Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-DIO-Ef1a-TVA-2A-oG

    Plasmid

    #172359

    Purpose

    Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling specifically from cre-expressing neurons

    Depositor

    Yoav Ben-Simon, Peter Jonas

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    TVA + B19-oG
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHage2-EF1a-TagBFP-hCoilin

    Plasmid

    #182589

    Purpose

    expresses bfp-hcoilin in mammalian cells

    Depositor

    Agnel Sfeir

    Article

    Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

    Insert

    Coilin (COIL Human)
    

    Use

    Lentiviral

    Tags

    BFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. miniTol2 x EF1a_EKAREN5 + PGK-puro

    Plasmid

    #167817

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN5
    

    Use

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-EF1a-Flp-DOG-NW

    Plasmid

    #75469

    Purpose

    Flp recombinase dependent on GFP (Flp-DOG) for rAAV production and expression in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

    Has Service

    AAV1

    Insert

    Flp-DOG
    

    Use

    AAV and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV x EF1a_EKAREN4-ires-blast

    Plasmid

    #167829

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN4
    

    Use

    Lentiviral

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-EF1a-jGCaMP8s-WPRE

    Plasmid

    #176758

    Purpose

    AAV-mediated expression of GCaMP8s under the EF1a promoter

    Depositor

    Loren Looger

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    jGCaMP8s
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAID1.2-EF1a-NGFP-mAID

    Plasmid

    #140607

    Purpose

    Expression of OsTIR1 and GFP-mAID protein in mammalian cells under the control of EF1a promoter

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    OsTIR1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-EF1a-FLEX-SomaGCaMP7

    Plasmid

    #182941

    Purpose

    Cre-dependent expression of cell body-targeted GCaMP7f, under EF1a promoter. As bright as conventional GCaMP7f.

    Depositor

    Edward Boyden

    Article

    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

    Insert

    Soma-GCaMP7
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAF0466 Ef1a-Nm60_attB acceptor

    Plasmid

    #193476

    Purpose

    Acceptor plasmid for 3 plasmid recombination assay

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Nm60_attB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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