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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 236,192 results for: ins

Showing: 741 - 760 of 236192 results

  1. pJBL-RNAINS4-A25C

    Plasmid
    #69461
    Purpose
    RNA-IN S4 A25C variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)
    Depositor
    Julius Lucks
    Article
    Watters et al Nucleic Acids Res. 2015 Sep 8. pii: gkv8
    Insert
    RNA-IN S4 with A25C mutation
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    A25C and mutations for S4 variant (from wt)
    Promoter
    J23119 and J23119 (sigma 70)
    Available since
    Sept. 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    69461-plasmid-map-sequence-id-122577

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  2. pJBL-RNAINS4-C24AA25C

    Plasmid
    #69463
    Purpose
    RNA-IN S4 C24AA25C variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)
    Depositor
    Julius Lucks
    Article
    Watters et al Nucleic Acids Res. 2015 Sep 8. pii: gkv8
    Insert
    RNA-IN S4 with C24A, A25C mutations
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    C24A, A25C, and mutations for S4 variant (from wt)
    Promoter
    J23119 and J23119 (sigma 70)
    Available since
    Sept. 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    69463-plasmid-map-sequence-id-122575

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  9. INS-2A-luciferase-2A-CD19-GFP

    Plasmid
    #159349
    Purpose
    Human INS targeting vector for knockin of 2A separated luciferase, humand CD19, and GFP. Targeted cells will be puromycin resistant.
    Depositor
    Rudolf Jaenisch
    Article
    Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d
    Inserts
    Luciferase-2A-human_CD19-2A-GFP
    Luciferase-2A-human_CD19-2A-GFP (CD19 Human)
    Use
    Ins locus knockin donor vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    159349-plasmid-map-sequence-id-313044

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  10. pcDNA3.1+CMV-Ins-GLuc

    Plasmid
    #89929
    Purpose
    Expresses Insulin-GLuc from the CMV promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.
    Depositor
    Melanie Cobb
    Article
    Kalwat et al ACS Sens. 2016 Oct 28;1(10):1208-1212. E
    Insert
    human insulin-Gaussia-Luciferase (INS Human, Synthetic)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    DNA sequence for Gaussia luciferase was humanized…
    Promoter
    CMV
    Available since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    89929-plasmid-map-sequence-id-255704

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  14. pINSPECT_5'-xrRNA_NLuc_3'-HCV-UTR

    Plasmid
    #197265
    Purpose
    Cloning Backbone for enhanced INSPECT expression (5' xrRNA + 3' HCV-UTR). Encodes for intronic IRES-driven NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via BsaI.
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676
    Type
    Empty backbone
    Use
    CRISPR, Luciferase, Synthetic Biology, and Unspec…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    197265-plasmid-map-sequence-id-389723

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  15. R777-E106 Hs.INSR-nostop

    Plasmid
    #70390
    Purpose
    Gateway ORF clone of human INSR [NM_001079817.2] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    INSR (INSR Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70390-plasmid-map-sequence-id-125839

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  16. proinsulin-msGFP2 (C48S C70S)

    Plasmid
    #218956
    Purpose
    Expresses proinsulin fused to msGFP2 (C48S C70S) in bacteria
    Depositor
    Benjamin Glick
    Insert
    proinsulin-msGFP2 C48S C70S
    Use
    Tags
    proinsulin
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    Sept. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    218956-plasmid-map-sequence-id-446162

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  18. INS-GFP-CD19-EPT

    Plasmid
    #210469
    Purpose
    Donor plasmid for knock-in GFP-CD19 into the human INS locus
    Depositor
    Kai Wang
    Article
    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do
    Insert
    INS homologous recombination arms with a GFP-CD19 and EF1alpha-drived puromycin-NLS-tdTomato selection cassette (INS Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    210469-plasmid-map-sequence-id-419052

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Showing: 741 - 760 of 236192 results