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pCAGGS-synPCB2.2

Plasmid

#139479

Purpose

Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

Insert

MTS-Fnr-Fd-P2A-MTS-HO1-PcyA-FLAG

Expression

Mammalian

Mutation

V554L

Promoter

CAG promoter

Available Since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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Tg 2 kb Violet opsin GFP

Plasmid

#72921

Purpose

violet opsin promoter from zebra finch (Taeniopygia guttata) gDNA (nucleotides21,946 to 145, corresponding to chr1A_ran-dom:206453–208444 in taeGut1) driving GFP

Depositor

Joseph Corbo

Article

Enright et al J Comp Neurol. 2015 Mar 1;523(4):649-68.

Insert

violet opsin promoter

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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Tg 2 kb Violet opsin DsRed

Plasmid

#72920

Purpose

violet opsin promoter from zebra finch (Taeniopygia guttata) gDNA (nucleotides21,946 to 145, corresponding to chr1A_ran-dom:206453–208444 in taeGut1) driving DsRed

Depositor

Joseph Corbo

Article

Enright et al J Comp Neurol. 2015 Mar 1;523(4):649-68.

Insert

violet opsin promoter

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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Ac 1.8kb red opsin GFP.

Plasmid

#72915

Purpose

red opsin promoter from Carolina anole lizard (Anolis carolinensis) gDNA (nucleotides21,797 to 187, corresponding to chr2:88663108–88664997 in anoCar2.0) driving GFP

Depositor

Joseph Corbo

Article

Enright et al J Comp Neurol. 2015 Mar 1;523(4):649-68.

Insert

red opsin promoter

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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Ac 1.8kb red opsin DsRed

Plasmid

#72914

Purpose

red opsin promoter from Carolina anole lizard (Anolis carolinensis) gDNA (nucleotides21,797 to 187, corresponding to chr2:88663108–88664997 in anoCar2.0) driving DsRed

Depositor

Joseph Corbo

Article

Enright et al J Comp Neurol. 2015 Mar 1;523(4):649-68.

Insert

red opsin promoter

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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Gg 1.8kb rhodopsin GFP

Plasmid

#72917

Purpose

Rhodopsin promoter from chicken (Gallus gallus) gDNA chr12:19,499,638–19,501,514 in galGal4 driving GFP

Depositor

Joseph Corbo

Article

Enright et al J Comp Neurol. 2015 Mar 1;523(4):649-68.

Insert

chicken rhodopsin (RHO Chicken)

Available Since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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Gg 1.8kb rhodopsin DsRed

Plasmid

#72916

Purpose

Rhodopsin promoter from chicken (Gallus gallus) gDNA chr12:19,499,638–19,501,514 in galGal4 driving DsRed

Depositor

Joseph Corbo

Article

Enright et al J Comp Neurol. 2015 Mar 1;523(4):649-68.

Insert

chicken rhodopsin (RHO Chicken)

Promoter

rhodopsin

Available Since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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Amber-free pNL4-3 FLuc Env-RT-

Plasmid

#238415

Purpose

An amber-free HIV NL4-3 carrying a Firefly luciferase reporter gene, RT and Env impaired.

Depositor

Maolin Lu

Article

Xu et al STAR Protoc. 2025 Jul 26;6(3):103995. do

Insert

Firefly luciferase (LOC116160065 Synthetic)

Use

Lentiviral and Luciferase

Expression

Mammalian

Mutation

Alternation of amber stop codons "TAG" …

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS_hTRPV1-p2A-tdTomato

Plasmid

#238923

Purpose

Expression of the human TRPV1 channel with tdTomato as a reporter in mammalian cells

Depositor

Vsevolod Belousov

Article

Maltsev et al Cell Mol Life Sci. 2024 Oct 25;81(1):437

Insert

hTRPV1-p2A-tdTomato

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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GfaABC1D-DIO-mNeonGreen-mScarlet-l-WPRE

Plasmid

#223668

Purpose

Cre-dependently expresses mNeonGreen, mScarlet-I in astrocytes

Depositor

Jun Nagai

Article

Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

Insert

mNeonGreen-mScarlet-l

Use

AAV and Cre/Lox

Promoter

GfaABC1D

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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mVenus-IQ6 R6A

Plasmid

#198202

Purpose

FRET acceptor: Mutated IQ6 domain of Moysin Va tagged with N-terminal mVenus (lower binding affinity)

Depositor

Christian Wahl-Schott

Article

Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

Insert

MYO5A IQ domain (Myo5a Mouse)

Tags

mVenus

Expression

Mammalian

Mutation

R6A

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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GfaABC1D-Lck-mCherry-ibARK

Plasmid

#241241

Purpose

Expression of plasma membrane-tethered ibARK in astrocytes

Depositor

Jun Nagai

Article

Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

Has Service

AAV5

Insert

Lck-mCherry-ibARK

Use

AAV

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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PZac2.1 gfaABC1D-NLS-SaCas9-3xHA-NLS bGH

Plasmid

#178960

Purpose

Expresses saCas9 protein specifically in astrocytes

Depositor

Baljit Khakh

Article

Endo et al Science. 2022 Nov 4;378(6619):eadc9020.

Insert

Staphylococcus aureus Cas9 (SaCas9)

Use

AAV

Tags

3x HA

Promoter

gfaABC1D (astrocyte specific)

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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RAM-d2TTA-TRE-hM4Di-mCherry-WPREpA

Plasmid

#223670

Purpose

AAV expression of hM4Di from RAM promoter

Depositor

Jun Nagai

Article

Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

Insert

hM4Di

Use

AAV

Tags

mCherry

Promoter

pRAM and TRE

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-LAMP1

Plasmid

#110948

Purpose

Expresses LAMP1 fused to mTurquoise2

Depositor

Pablo Rivera-Fuentes

Article

Halabi et al Nat Commun. 2019 Mar 15;10(1):1232. doi:

Insert

Lysosomal-associated membrane protein 1 (LAMP1 Human)

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pmStrawberry-Atg4BC74A

Plasmid

#21076

Depositor

Tamotsu Yoshimori

Article

Fujita et al Mol Biol Cell. 2008 Nov . 19(11):4651-9.

Insert

Atg4B(C74A) (Atg4b Mouse)

Tags

mStrawberry

Expression

Mammalian

Mutation

220-222 TGC(Cys) is changed to GCA (Ala)

Available Since

June 8, 2009

Availability

Academic Institutions and Nonprofits only

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pT2Apuro-TRE-synPCB2.0

Plasmid

#139481

Purpose

Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

Insert

MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7

Use

Tol2 transposon donor vector

Expression

Mammalian

Promoter

Tet responsive element

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pGTM_COV2_NSP5_004_SUMO

Plasmid

#190062

Purpose

Expresses SARS-CoV-2 3CLpro for the expression of the active mature protease

Depositor

Gaetano Montelione

Article

Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi

Insert

3CLpro (ORF1ab SARS-CoV-2)

Tags

6X-His-Sumo

Expression

Bacterial

Promoter

T7 lac

Available Since

June 3, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pET30a-GABAT

Plasmid

#239572

Purpose

Expresses E. coli GABA transaminase (GABA-T/gabT) for recombinant protein purification. Contains a His6-tag on its C-terminus for Ni2+ affinity chromatography.

Depositor

William Plaxton

Article

Benidickson et al Biol Methods Protoc. 2025 Jun 20;10(1):b

Insert

4-aminobutyrate aminotransferase (gabT E. coli)

Tags

His6

Expression

Bacterial

Available Since

July 18, 2025

Availability

Academic Institutions and Nonprofits only

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pX330_VCL sgRNA / hSpCas9

Plasmid

#172837

Purpose

Mammalian expression of a sgRNA targeting the intron 21 (last intron) of VCL (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

sgRNA targeting intron 21 of VCL under the U6 promotor and hSpCas9 under the CAG promotor

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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