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pCMV-dLight1.3a

Plasmid

#111055

Purpose

Expresses dLight1.3a in mammalian cells

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Insert

dLight1.3a

Tags

Flag tag

Expression

Mammalian

Promoter

CAG

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pVC297 VEGF Site#1

Plasmid

#47505

Purpose

human gRNA expression vector targeting VEGF

Depositor

Keith Joung

Article

Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

Insert

gRNA-VEGF site 1 (VEGFA Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pTBL437 AAVS1 EQR sgRNA

Plasmid

#126447

Purpose

To cut the human AAVS1 locus for integration.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human AAVS1 safe-harbor locus with NGAG PAM

Expression

Mammalian

Promoter

human U6

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pD21-hgAPOAI

Plasmid

#124962

Purpose

Express ApoAI in the liver

Depositor

Kazuhiro Oka

Article

Belalcazar et al Circulation. 2003 Jun 3;107(21):2726-32.

Insert

human APOAI gene (LPA Human)

Use

Adenoviral

Promoter

human APOAI

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-Cbir-Cdc25C WT_Myc-Nbir-GFP

Plasmid

#90012

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C and N-terminally tagged NBir[2-256]-GFP

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

CBirA*-Cdc25C
NBirA*-GFP

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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LSB-hsa-miR-101-3p

Plasmid

#103165

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-101-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-101-3p target

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF-1a

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223-MCU-S259A

Plasmid

#31728

Depositor

Vamsi Mootha

Article

Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

Insert

MCU (MCU Human)

Use

Pdonr gateway

Tags

None

Mutation

Changed S259 to Alanine

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only
pVC228 VEGF Site#3

Plasmid

#47507

Purpose

human gRNA expression vector targeting VEGF

Depositor

Keith Joung

Article

Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

Insert

gRNA-VEGF site 3 (VEGFA Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag MKK7a1 (ala)

Plasmid

#14539

Depositor

Roger Davis

Article

Tournier et al Proc Natl Acad Sci U S A. 1997 Jul 8. 94

Insert

MKK7a1(Ala) (Map2k7 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Ser-198Ala Thr-202Ala

Available Since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
pCXLE-hK + E2-Crimson

Plasmid

#74946

Purpose

Dosage control and tracing of reprogramming episomes

Depositor

Ann Zovein

Article

Schmitt et al Stem Cell Res Ther. 2017 Jun 5;8(1):132.

Insert

Klf4 (KLF4 Human)

Expression

Mammalian

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only
pAS_4xhyb PylT A41AA C55A sfGFP 102TAA 150TAG

Plasmid

#154777

Purpose

plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and dual suppression reporter sfGFP 102TAA 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

sfGFP

Expression

Mammalian

Mutation

102TAA 150TAG in GFP reporter, hybrid PylT with …

Promoter

EF1

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX PLCg1(C)-SH2

Plasmid

#46469

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

PLCg1(C)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 656-766

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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inhibin-DsRed

Plasmid

#156184

Purpose

Expression plasmid for production of transgenic rainbow trout strain carrying the DsRed gene under control of the inhibin α promoter.

Depositor

Goro Yoshizaki

Article

Iwasaki-Takahashi et al Commun Biol. 2020 Jun 15;3(1):308. doi:

Inserts

rainbow trout inhibin α promoter
rainbow trout β actin 3'UTR

Expression

Bacterial

Promoter

inhibin

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pAS_4xG1 PylT FLAG-G1 PylRS

Plasmid

#154768

Purpose

plasmid with 4xG1 PylT cassette and G1 PylRS for amber suppression in transient or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)

Tags

FLAG

Expression

Mammalian

Promoter

EF1

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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YFP-Stim1-ΔCTD

Plasmid

#186621

Purpose

Encodes Stim1 with C-terminal domain deletion fused to YFP

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Stromal Interaction Molecule 1 (STIM1 Human)

Tags

YFP

Expression

Mammalian

Mutation

Deletion of C-terminal domain 601 to 685

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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200 bp Axin2 basic

Plasmid

#25703

Depositor

Eric Fearon

Article

Leung et al J Biol Chem. 2002 Jun 14. 277(24):21657-

Insert

Axin 2 promoter (AXIN2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag MKK7a1 (K76A)

Plasmid

#14541

Depositor

Roger Davis

Article

Tournier et al Proc Natl Acad Sci U S A. 1997 Jul 8. 94

Insert

MKK7a1(K76A) (Map2k7 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Lys-76Ala

Available Since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3-mCherry-TSC2(AA)-NLS

Plasmid

#192443

Purpose

Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation; targeted to the nucleus.

Depositor

Jin Zhang

Article

Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

Insert

mCherry-TSC2(S939A,T1439A)-NLS (TSC2 Human)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

Promoter

CMV

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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L-CRISPR-CTN (MLL-ctrl)

Plasmid

#69215

Purpose

Advanced lentiviral CRISPR-Cas9 vector for induction of chromosomal translocations; control, MLL-sgRNA + luciferase-sgRNA

Depositor

Dirk Heckl

Article

Reimer et al Haematologica. 2017 Jun 1. pii: haematol

Inserts

SpCas9
Sp sgRNA scaffold
SFFV promoter
P2A-mNeonGreen

Use

CRISPR and Lentiviral

Tags

FLAG

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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RNAseq_cal_Tuschl

Plasmid

#59426

Purpose

Encodes sequence non-cognate to human genome for generation of RNAseq calibrators

Depositor

Thomas Tuschl

Article

Max et al Proc Natl Acad Sci U S A. 2018 Jun 5;115

Insert

RNAseq_cal_Tuschl

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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