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pILGFP77

Plasmid

#83538

Purpose

Used to evaluate the expression output of RPL8B promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

RPL8B promoter-yEGFP

Expression

Yeast

Promoter

RPL8B

Available Since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pTCW018

Plasmid

#83531

Purpose

Aromatic amino acid inducible expression of alpha pheromone, and pheromone inducible exression of an ARO7 RNA interference hairpin

Depositor

Claudia Vickers

Article

Williams et al Metab Eng. 2015 May;29:124-134. doi: 10.

Insert

pARO9-mf_2-pFUS1J2-ARO7i-CYC1t

Expression

Yeast

Promoter

pARO9/pFUS1J2

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pILGFPH0

Plasmid

#83552

Purpose

Used to evaluate the expression output of TPS1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

TPS1 promoter-yEGFP

Expression

Yeast

Promoter

TPS1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pILGFP90

Plasmid

#83547

Purpose

Used to evaluate the expression output of TEF2 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

TEF2 promoter-yEGFP

Expression

Yeast

Promoter

TEF2

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pILGFP82

Plasmid

#83541

Purpose

Used to evaluate the expression output of YEF3 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

YEF3 promoter-yEGFP

Expression

Yeast

Promoter

YEF3

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pILGFP80

Plasmid

#83540

Purpose

Used to evaluate the expression output of RPL3promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

RPL3 promoter-yEGFP

Expression

Yeast

Promoter

RPL3

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pILGFP78

Plasmid

#83539

Purpose

Used to evaluate the expression output of SSB1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

SSB1 promoter-yEGFP

Expression

Yeast

Promoter

SSB1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMV-TRP1

Plasmid

#65335

Purpose

standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker TRP1 which could be released and ligated to series of TUs

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TRP1

Use

Synthetic Biology

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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Gal4pBS3-Pleum

Plasmid

#60330

Purpose

Contains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Expression

Yeast

Available Since

Feb. 11, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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Gal4pBS1-Pleum

Plasmid

#59993

Purpose

Contains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Expression

Yeast

Available Since

Feb. 11, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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Tpa1(21-644)

Plasmid

#23140

Depositor

SeWon Suh

Article

Kim et al Nucleic Acids Res. 2010 Apr;38(6):2099-1

Insert

Tpa1 (TPA1 Budding Yeast)

Tags

His

Expression

Bacterial

Mutation

deleted amino acids 1-20

Available Since

Sept. 20, 2010

Availability

Academic Institutions and Nonprofits only
Tpa1(273-644)

Plasmid

#23141

Depositor

SeWon Suh

Article

Kim et al Nucleic Acids Res. 2010 Apr;38(6):2099-1

Insert

Tpa1 C-terminal domain (TPA1 Budding Yeast)

Tags

His

Expression

Bacterial

Mutation

deleted amino acids 1-272

Available Since

Sept. 20, 2010

Availability

Academic Institutions and Nonprofits only
Dom34(265-377)

Plasmid

#19103

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Protein DOM34 (DOM34 Budding Yeast)

Tags

C tag and N tag

Expression

Bacterial

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
FLAG-SpELL pNMT-TOPO

Plasmid

#15315

Depositor

Joan Conaway, Ronald Conaway

Article

Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

Insert

eleven nineteen lysine rich in leukemia (ell1 Fission Yeast)

Tags

FLAG

Expression

Yeast

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
FLAG-SpEAF pNMT-TOPO

Plasmid

#15316

Depositor

Joan Conaway, Ronald Conaway

Article

Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

Insert

ELL associated factor (eaf1 Fission Yeast)

Tags

FLAG

Expression

Yeast

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
FLAG-SpELL pBacPAK8

Plasmid

#15317

Depositor

Joan Conaway, Ronald Conaway

Article

Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

Insert

eleven nineteen lysine rich in leukemia (ell1 Fission Yeast)

Use

Baculovirus construction

Tags

FLAG

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3-LifeAct-FRB-DMVF-181-N

Plasmid

#87610

Purpose

FRB-tagged N-terminal Dronpa fragment for BiFC; localizes to actin cytoskeleton.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

LifeAct-FRB-DMVF181-N

Tags

17-aa sequence from Abp140 (aka "Lifeact&quo…

Expression

Mammalian

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pDonor_e-attP-H1-g3-Ef1a-Puro-P2A-mCherry

Plasmid

#247160

Purpose

Engineered e-attP donor plasmid expressing puromycin resistance and mCherry with donor binding sgRNA H1-g3

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

Puromycin resistance, mCherry

Expression

Mammalian

Promoter

Ef1a

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pAAVS1-P-CAG-GFP

Plasmid

#80491

Purpose

donor vector for AAVS1 targeting (puromycin selection) and constitutive GFP expression

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Insert

CAG-GFP

Use

Donor vector (human)

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-ATG8(416)/GFP-AUT7(416)

Plasmid

#49425

Purpose

Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS416 vector.

Depositor

Daniel Klionsky

Article

Guan et al Mol Biol Cell. 2001 Dec;12(12):3821-38.

Insert

autophagy-related 8 (ATG8 Budding Yeast)

Tags

GFP

Expression

Bacterial and Yeast

Promoter

ATG8

Available Since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only

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